Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CSB90_RS06955 Genome accession   NZ_CP027172
Coordinates   1463184..1463648 (+) Length   154 a.a.
NCBI ID   WP_105446987.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AR_0353     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1458184..1468648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSB90_RS06930 (CSB90_1401) yacG 1458349..1458549 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  CSB90_RS06935 (CSB90_1402) coaE 1458546..1459157 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  CSB90_RS06940 (CSB90_1403) pilD 1459154..1460026 (-) 873 WP_003134888.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CSB90_RS06945 (CSB90_1404) pilC 1460030..1461250 (-) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  CSB90_RS06950 (CSB90_1405) pilB 1461254..1462954 (-) 1701 WP_003094647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CSB90_RS06955 pilA 1463184..1463648 (+) 465 WP_105446987.1 pilin Machinery gene
  CSB90_RS06965 (CSB90_1407) nadC 1463851..1464699 (-) 849 WP_003125200.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CSB90_RS06970 (CSB90_1408) - 1464985..1467240 (+) 2256 WP_003134886.1 DUF1631 domain-containing protein -
  CSB90_RS06975 (CSB90_1409) ampD 1467391..1467957 (+) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15820.93 Da        Isoelectric Point: 6.8958

>NTDB_id=230898 CSB90_RS06955 WP_105446987.1 1463184..1463648(+) (pilA) [Pseudomonas aeruginosa strain AR_0353]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVSRSQVSRVMAEAGSLKTAVEACLQDGRTAVGTAAGQCDPGATGSSL
LTGASQTSQTLPTNTGVPQVLDHLTTQTTIIATFGNGASAAISGQTLTWTRDVNGGWSCATTVDAKFRPNGCTD

Nucleotide


Download         Length: 465 bp        

>NTDB_id=230898 CSB90_RS06955 WP_105446987.1 1463184..1463648(+) (pilA) [Pseudomonas aeruginosa strain AR_0353]
ATGAAAGCACAGAAGGGTTTTACTCTGATTGAACTGATGATCGTAGTTGCGATCATTGGTATTCTGGCTGCAATCGCAAT
TCCCCAATATCAGAACTATGTTTCTCGCTCTCAGGTCTCCAGGGTTATGGCAGAGGCTGGCTCCTTGAAGACTGCAGTTG
AGGCCTGCCTCCAGGATGGTCGTACTGCTGTGGGTACTGCTGCTGGTCAATGCGACCCGGGTGCGACGGGTTCCAGTTTG
TTGACTGGTGCTTCTCAGACTTCTCAAACCCTGCCAACCAATACCGGTGTTCCGCAGGTTCTGGATCATCTGACTACTCA
AACCACTATCATTGCGACTTTTGGTAACGGTGCATCCGCAGCTATTTCTGGCCAGACTCTGACCTGGACTCGTGATGTTA
ATGGTGGCTGGAGCTGTGCTACTACCGTAGATGCTAAATTCCGTCCTAATGGCTGTACTGACTGA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-153)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

46.753

100

0.468
  pilA Vibrio cholerae strain A1552

43.59

100

0.442
  pilA Vibrio cholerae C6706

43.59

100

0.442
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.59

100

0.442
  comP Acinetobacter baylyi ADP1

40.12

100

0.435
  pilA Vibrio parahaemolyticus RIMD 2210633

40.881

100

0.422
  pilA Acinetobacter baumannii strain A118

41.83

99.351

0.416
  pilA2 Legionella pneumophila strain ERS1305867

41.558

100

0.416
  pilA2 Legionella pneumophila str. Paris

40.909

100

0.409
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40

100

0.403
  pilE Neisseria gonorrhoeae MS11

33.908

100

0.383
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.411

98.052

0.377