Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   THSYN_RS26785 Genome accession   NZ_CP020370
Coordinates   6248059..6248478 (+) Length   139 a.a.
NCBI ID   WP_100921821.1    Uniprot ID   A0A2K8UF33
Organism   Candidatus Thiodictyon syntrophicum strain Cad16T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6243059..6253478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THSYN_RS26765 (THSYN_26575) acnD 6243237..6245831 (-) 2595 WP_100921817.1 Fe/S-dependent 2-methylisocitrate dehydratase AcnD -
  THSYN_RS26770 (THSYN_26580) - 6246255..6246512 (+) 258 WP_100921818.1 FitA-like ribbon-helix-helix domain-containing protein -
  THSYN_RS26775 (THSYN_26585) - 6246509..6246934 (+) 426 WP_100921819.1 type II toxin-antitoxin system VapC family toxin -
  THSYN_RS26780 (THSYN_26590) - 6247098..6247652 (+) 555 WP_100921820.1 hypothetical protein -
  THSYN_RS26785 (THSYN_26595) pilA2 6248059..6248478 (+) 420 WP_100921821.1 pilin Machinery gene
  THSYN_RS26790 (THSYN_26600) - 6248802..6250802 (+) 2001 WP_100921822.1 hypothetical protein -
  THSYN_RS34380 - 6250906..6251043 (+) 138 WP_157817961.1 hypothetical protein -
  THSYN_RS26795 (THSYN_26605) - 6251040..6251906 (+) 867 WP_100921823.1 glycosyltransferase family 2 protein -
  THSYN_RS26800 (THSYN_26610) - 6251903..6252874 (+) 972 WP_100921824.1 hypothetical protein -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14370.54 Da        Isoelectric Point: 10.1061

>NTDB_id=221836 THSYN_RS26785 WP_100921821.1 6248059..6248478(+) (pilA2) [Candidatus Thiodictyon syntrophicum strain Cad16T]
MKKYQQGFTLIELMIVVAIIGILAAIAIPSYQDFTTRSKVAEGLNLAGSPKASAAEFALNNGHWPASGVGSLGYQSPTTK
YVRSIAVAGSHITITYAGTAGVVAGSNIIIAGSLNAGGAKWVCTGGNVNIRYRPSNCRP

Nucleotide


Download         Length: 420 bp        

>NTDB_id=221836 THSYN_RS26785 WP_100921821.1 6248059..6248478(+) (pilA2) [Candidatus Thiodictyon syntrophicum strain Cad16T]
ATGAAAAAGTACCAACAAGGCTTCACCTTGATAGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGGCCATCGC
CATCCCGTCCTACCAAGATTTCACCACACGCAGCAAGGTGGCGGAAGGACTCAACCTCGCTGGCTCGCCGAAAGCTTCGG
CGGCCGAATTTGCGCTCAATAATGGCCATTGGCCGGCAAGTGGTGTCGGGAGTCTCGGCTACCAGTCCCCGACGACCAAA
TATGTTCGGAGTATCGCCGTCGCCGGAAGCCACATCACGATCACGTATGCGGGCACCGCGGGCGTAGTGGCTGGCAGCAA
CATCATCATCGCCGGCTCGCTGAACGCAGGCGGCGCCAAGTGGGTGTGCACCGGCGGCAACGTTAACATCAGATATCGGC
CGTCCAACTGCCGGCCATAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K8UF33

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

55.072

99.281

0.547
  pilA2 Legionella pneumophila str. Paris

54.348

99.281

0.54
  pilA Ralstonia pseudosolanacearum GMI1000

39.394

100

0.468
  pilE Neisseria gonorrhoeae strain FA1090

38.509

100

0.446
  comP Acinetobacter baylyi ADP1

41.096

100

0.432
  pilE Neisseria gonorrhoeae MS11

37.5

100

0.432
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.958

100

0.432
  pilA Pseudomonas aeruginosa PAK

38.667

100

0.417
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40

100

0.403
  pilA Vibrio cholerae C6706

37.5

100

0.388
  pilA Vibrio cholerae strain A1552

37.5

100

0.388
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.5

100

0.388
  pilA/pilA1 Eikenella corrodens VA1

34.868

100

0.381

Multiple sequence alignment