Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   RD110_RS26355 Genome accession   NZ_CP019236
Coordinates   5710782..5711222 (-) Length   146 a.a.
NCBI ID   WP_076203897.1    Uniprot ID   A0A1P8K2P0
Organism   Rhodoferax koreense strain DCY-110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5705782..5716222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RD110_RS26335 (RD110_26335) moaC 5705862..5706341 (+) 480 WP_076203886.1 cyclic pyranopterin monophosphate synthase MoaC -
  RD110_RS26340 (RD110_26340) - 5706338..5707288 (-) 951 WP_076203888.1 IS481 family transposase -
  RD110_RS26345 (RD110_26345) - 5707387..5708817 (-) 1431 WP_239467133.1 Wzy polymerase domain-containing protein -
  RD110_RS26350 (RD110_26350) - 5708937..5710616 (-) 1680 WP_076203894.1 PglL family O-oligosaccharyltransferase -
  RD110_RS26355 (RD110_26355) pilE 5710782..5711222 (-) 441 WP_076203897.1 pilin Machinery gene
  RD110_RS26360 (RD110_26360) - 5711382..5712173 (-) 792 WP_076203900.1 3',5'-cyclic-nucleotide phosphodiesterase -
  RD110_RS26365 (RD110_26365) - 5712183..5714435 (-) 2253 WP_338053078.1 adenylate/guanylate cyclase domain-containing protein -
  RD110_RS26370 (RD110_26370) - 5714505..5715203 (-) 699 WP_076203902.1 FHA domain-containing protein -
  RD110_RS26375 (RD110_26375) - 5715246..5716031 (-) 786 WP_076205754.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15036.27 Da        Isoelectric Point: 9.0088

>NTDB_id=214326 RD110_RS26355 WP_076203897.1 5710782..5711222(-) (pilE) [Rhodoferax koreense strain DCY-110]
MKRSLQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVSEVILAASSAKTSLSEYAQVNGQMPSDTSSVDTQSSAFV
TSVTYARTNASVGVVTVTASAKEPKISGKTLTLTGTLGSTGQVSWVCGPGATSGIDVKYLPASCKP

Nucleotide


Download         Length: 441 bp        

>NTDB_id=214326 RD110_RS26355 WP_076203897.1 5710782..5711222(-) (pilE) [Rhodoferax koreense strain DCY-110]
ATGAAGCGTTCTCTGCAACAAGGTTTCACCCTCATCGAGTTGATGATCGTCGTGGCGATCATCGGTATTTTGGCTGCCGT
GGCTTTGCCGGCTTATCAGGATTACACGGTGCGGGCGAAGGTGTCTGAAGTGATTCTGGCCGCTTCATCGGCAAAAACTT
CGCTGTCCGAATACGCTCAGGTCAATGGTCAAATGCCCTCCGACACAAGCTCCGTTGACACGCAATCATCTGCTTTCGTG
ACCAGCGTCACGTATGCTCGGACCAATGCATCTGTCGGCGTGGTTACGGTCACGGCTTCGGCAAAAGAACCAAAGATCAG
TGGCAAAACGTTGACCCTGACGGGGACATTGGGGAGCACGGGCCAAGTCTCTTGGGTGTGTGGACCAGGCGCGACTTCTG
GTATCGACGTGAAGTATCTACCTGCAAGCTGCAAACCCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)

Pilin (bacterial filament) (Pilin)

(37-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1P8K2P0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.153

100

0.507
  pilE Neisseria gonorrhoeae MS11

43.396

100

0.473
  pilA Ralstonia pseudosolanacearum GMI1000

39.766

100

0.466
  comP Acinetobacter baylyi ADP1

45.333

100

0.466
  pilA2 Legionella pneumophila strain ERS1305867

47.518

96.575

0.459
  pilA2 Legionella pneumophila str. Paris

47.518

96.575

0.459
  pilA/pilA1 Eikenella corrodens VA1

41.25

100

0.452
  pilE Neisseria gonorrhoeae strain FA1090

41.139

100

0.445
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.568

100

0.432
  pilA Vibrio campbellii strain DS40M4

37.342

100

0.404
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.136

100

0.404
  pilA Acinetobacter baumannii strain A118

37.5

98.63

0.37

Multiple sequence alignment