Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   BJD13_RS22570 Genome accession   NZ_CP018475
Coordinates   4689411..4689803 (+) Length   130 a.a.
NCBI ID   WP_074053364.1    Uniprot ID   -
Organism   Xanthomonas perforans strain LH3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4690360..4698874 4689411..4689803 flank 557


Gene organization within MGE regions


Location: 4689411..4698874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJD13_RS22570 (BJD13_22700) pilA/pilAI 4689411..4689803 (+) 393 WP_074053364.1 pilin Machinery gene
  BJD13_RS22575 (BJD13_22705) - 4689896..4690315 (+) 420 WP_033479197.1 pilin -
  BJD13_RS22580 (BJD13_22710) - 4690360..4692192 (+) 1833 WP_008576878.1 hypothetical protein -
  BJD13_RS22585 (BJD13_22715) - 4692314..4693012 (+) 699 WP_158070652.1 class I SAM-dependent methyltransferase -
  BJD13_RS24830 (BJD13_22720) - 4693009..4693923 (+) 915 WP_126952614.1 lysylphosphatidylglycerol synthase domain-containing protein -
  BJD13_RS24370 - 4693915..4695048 (-) 1134 WP_161797462.1 glycosyltransferase -
  BJD13_RS22600 (BJD13_22730) - 4695074..4695931 (-) 858 WP_074053368.1 glycosyltransferase family 2 protein -
  BJD13_RS22605 (BJD13_22735) pilB 4696188..4697924 (+) 1737 WP_008575094.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 130 a.a.        Molecular weight: 13591.81 Da        Isoelectric Point: 9.5775

>NTDB_id=208860 BJD13_RS22570 WP_074053364.1 4689411..4689803(+) (pilA/pilAI) [Xanthomonas perforans strain LH3]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQVTAGLAEITPGKTQFEVKTNEGSTMSTPAQIGLSSSTTRCGI
TVSATTIVCNLKGNAAKVKDKNVTLTRTASTGTWACSSSVDPKYKPAGCS

Nucleotide


Download         Length: 393 bp        

>NTDB_id=208860 BJD13_RS22570 WP_074053364.1 4689411..4689803(+) (pilA/pilAI) [Xanthomonas perforans strain LH3]
ATGAAGAAGCAGCAAGGCTTTACGCTGATCGAACTGATGATCGTGGTCGCGATCATTGCCATCCTGGCCGCCATCGCGCT
GCCGATGTATCAGGACTATGTCGCCAAGTCGCAGGTGACTGCTGGTCTGGCCGAAATCACCCCGGGCAAGACTCAGTTCG
AAGTTAAGACCAACGAAGGCAGCACGATGAGCACCCCCGCGCAGATTGGTCTAAGCTCGTCCACCACGCGTTGTGGCATC
ACGGTTTCCGCTACTACCATTGTTTGCAACCTTAAAGGAAACGCGGCAAAGGTCAAGGATAAGAACGTGACACTGACGCG
TACCGCTAGCACGGGAACTTGGGCTTGCAGCAGCAGTGTTGACCCGAAGTACAAGCCGGCTGGCTGCAGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-129)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

54.815

100

0.569
  pilA Acinetobacter baumannii strain A118

46.763

100

0.5
  pilA/pilAII Pseudomonas stutzeri DSM 10701

47.368

100

0.485
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.056

100

0.477
  pilA Vibrio cholerae strain A1552

43.056

100

0.477
  pilA Vibrio cholerae C6706

43.056

100

0.477
  pilA Pseudomonas aeruginosa PAK

41.611

100

0.477
  comP Acinetobacter baylyi ADP1

40.541

100

0.462
  pilA Vibrio parahaemolyticus RIMD 2210633

41.304

100

0.438
  pilA/pilA1 Eikenella corrodens VA1

36.242

100

0.415
  pilA2 Legionella pneumophila str. Paris

37.241

100

0.415
  pilA2 Legionella pneumophila strain ERS1305867

37.241

100

0.415
  pilE Neisseria gonorrhoeae strain FA1090

30.719

100

0.362

Multiple sequence alignment