Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   BFM96_RS04200 Genome accession   NZ_CP016953
Coordinates   871544..872485 (+) Length   313 a.a.
NCBI ID   WP_068990723.1    Uniprot ID   A0A917A8W2
Organism   Streptococcus himalayensis strain HTS2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 866544..877485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFM96_RS04185 - 867213..868469 (+) 1257 WP_068990719.1 ISL3 family transposase -
  BFM96_RS04190 - 868547..870916 (-) 2370 WP_083201742.1 bifunctional 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase -
  BFM96_RS04195 - 871117..871482 (+) 366 WP_068990721.1 DUF1033 family protein -
  BFM96_RS04200 comGA/cglA/cilD 871544..872485 (+) 942 WP_068990723.1 competence type IV pilus ATPase ComGA Machinery gene
  BFM96_RS04205 comYB 872406..873449 (+) 1044 WP_068994164.1 competence type IV pilus assembly protein ComGB Machinery gene
  BFM96_RS04210 comGC/cglC 873461..873781 (+) 321 WP_068994166.1 competence type IV pilus major pilin ComGC Machinery gene
  BFM96_RS04215 comYD 873792..874169 (+) 378 WP_068990726.1 competence type IV pilus minor pilin ComGD Machinery gene
  BFM96_RS04220 comGE/cglE 874141..874431 (+) 291 WP_068990728.1 competence type IV pilus minor pilin ComGE Machinery gene
  BFM96_RS04225 comGF/cglF 874397..874855 (+) 459 WP_145939743.1 competence type IV pilus minor pilin ComGF Machinery gene
  BFM96_RS04230 comGG 874827..875291 (+) 465 WP_068990735.1 competence type IV pilus minor pilin ComGG -
  BFM96_RS04235 comYH 875335..876282 (+) 948 WP_068990737.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35878.22 Da        Isoelectric Point: 6.8275

>NTDB_id=194010 BFM96_RS04200 WP_068990723.1 871544..872485(+) (comGA/cglA/cilD) [Streptococcus himalayensis strain HTS2]
MVQEIAQEIIKQAREKVAQDIYFIPKEDCYELYMRIGDERRLIGQYDFEKFAAVISHFKYRAGMNVGEKRRSQLGSCDYP
CGQEEVSLRLSTVGDYRGLESLVIRLLHDKKSQLHFWFADLKEVSKIVKQRGLYLFSGPVGSGKTTLMHYLAQMRFKGQQ
IMSIEDPVEIKQDTMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDAETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEDELRIVLQGVCYQRLIKGGGLVDFANQHYQEHQPEVWNAQIDQLFAAGHISFEQAESEKIIHQ

Nucleotide


Download         Length: 942 bp        

>NTDB_id=194010 BFM96_RS04200 WP_068990723.1 871544..872485(+) (comGA/cglA/cilD) [Streptococcus himalayensis strain HTS2]
ATGGTTCAAGAAATTGCACAAGAGATTATCAAGCAGGCTAGAGAAAAAGTAGCTCAGGATATTTACTTTATCCCAAAAGA
GGATTGTTATGAACTCTATATGCGAATTGGCGATGAACGCCGATTGATTGGGCAATATGATTTTGAAAAATTTGCAGCAG
TCATCAGCCATTTTAAGTATAGAGCGGGGATGAATGTTGGAGAAAAACGTCGCAGTCAGTTGGGCTCCTGTGATTATCCT
TGTGGACAAGAGGAAGTATCTCTACGTTTATCCACAGTTGGAGATTATCGAGGTCTAGAGAGTCTTGTGATTCGCTTGCT
TCATGATAAAAAATCCCAACTTCACTTTTGGTTTGCGGATTTGAAAGAAGTGAGCAAGATTGTGAAACAACGTGGCCTCT
ATCTTTTTTCGGGACCGGTAGGAAGTGGGAAAACGACTTTAATGCACTATTTGGCACAGATGCGTTTTAAAGGGCAACAG
ATTATGTCTATCGAAGATCCTGTGGAAATTAAGCAGGATACGATGCTCCAGCTCCAACTCAATGAAGCGATTGGCTTGAC
CTATGAAAATCTCATCAAGCTATCCTTACGCCATCGGCCAGACTTGCTCATCATTGGGGAAATTCGAGATGCTGAAACGG
CACGGGCAGTTGTCAGAGCAAGCTTGACGGGAGCAACTGTTTTTTCAACCATTCACGCTAAGAGCATCCGTGGGGTGTAT
GAACGTTTGCTCGAGTTGGGTGTGAGCGAGGATGAACTGCGGATTGTGCTACAAGGCGTATGTTATCAACGATTGATCAA
GGGAGGAGGCCTAGTCGATTTTGCAAACCAACACTATCAAGAGCACCAGCCAGAAGTCTGGAATGCGCAAATTGATCAGC
TTTTTGCAGCAGGACATATCAGTTTTGAACAAGCGGAAAGCGAAAAAATTATCCATCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

81.29

99.042

0.805
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

79.677

99.042

0.789
  comGA/cglA/cilD Streptococcus pneumoniae D39

79.677

99.042

0.789
  comGA/cglA/cilD Streptococcus pneumoniae R6

79.677

99.042

0.789
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

79.677

99.042

0.789
  comYA Streptococcus gordonii str. Challis substr. CH1

78.275

100

0.783
  comYA Streptococcus mutans UA159

64.63

99.361

0.642
  comYA Streptococcus mutans UA140

64.63

99.361

0.642
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

64.194

99.042

0.636
  comGA Lactococcus lactis subsp. cremoris KW2

54.662

99.361

0.543
  comGA Latilactobacillus sakei subsp. sakei 23K

41.135

90.096

0.371

Multiple sequence alignment