Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   BFM96_RS04225 Genome accession   NZ_CP016953
Coordinates   874397..874855 (+) Length   152 a.a.
NCBI ID   WP_145939743.1    Uniprot ID   -
Organism   Streptococcus himalayensis strain HTS2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 869397..879855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFM96_RS04195 - 871117..871482 (+) 366 WP_068990721.1 DUF1033 family protein -
  BFM96_RS04200 comGA/cglA/cilD 871544..872485 (+) 942 WP_068990723.1 competence type IV pilus ATPase ComGA Machinery gene
  BFM96_RS04205 comYB 872406..873449 (+) 1044 WP_068994164.1 competence type IV pilus assembly protein ComGB Machinery gene
  BFM96_RS04210 comGC/cglC 873461..873781 (+) 321 WP_068994166.1 competence type IV pilus major pilin ComGC Machinery gene
  BFM96_RS04215 comYD 873792..874169 (+) 378 WP_068990726.1 competence type IV pilus minor pilin ComGD Machinery gene
  BFM96_RS04220 comGE/cglE 874141..874431 (+) 291 WP_068990728.1 competence type IV pilus minor pilin ComGE Machinery gene
  BFM96_RS04225 comGF/cglF 874397..874855 (+) 459 WP_145939743.1 competence type IV pilus minor pilin ComGF Machinery gene
  BFM96_RS04230 comGG 874827..875291 (+) 465 WP_068990735.1 competence type IV pilus minor pilin ComGG -
  BFM96_RS04235 comYH 875335..876282 (+) 948 WP_068990737.1 class I SAM-dependent methyltransferase Machinery gene
  BFM96_RS04240 - 876341..877531 (+) 1191 WP_068990741.1 acetate kinase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 17404.94 Da        Isoelectric Point: 9.5629

>NTDB_id=194015 BFM96_RS04225 WP_145939743.1 874397..874855(+) (comGF/cglF) [Streptococcus himalayensis strain HTS2]
MERRKSSVWKNKKLRAFTLLEALVSLLVISGSVLLFQSMAQLLASEVQAQKNNDIKEWLLFADQLDAELSRSQFDKVENG
KLYVTQGGKGIAFGKSNKDDFRKTDSSGRGYQPMLYGLKEAQITKEESLIHIHLQFEKGLEREYVYRVEEKG

Nucleotide


Download         Length: 459 bp        

>NTDB_id=194015 BFM96_RS04225 WP_145939743.1 874397..874855(+) (comGF/cglF) [Streptococcus himalayensis strain HTS2]
ATGGAAAGGAGGAAATCATCCGTGTGGAAAAATAAGAAGTTACGAGCTTTCACGCTCCTAGAAGCTTTGGTTTCCCTTCT
AGTTATCAGCGGTAGTGTCCTCCTTTTTCAGTCCATGGCCCAACTCCTTGCGTCTGAAGTTCAGGCACAGAAAAATAATG
ACATCAAGGAATGGTTGCTTTTTGCGGATCAGTTAGATGCTGAACTCAGCCGAAGTCAGTTTGACAAGGTTGAAAATGGT
AAACTATATGTAACCCAAGGTGGCAAAGGGATTGCTTTTGGCAAGTCTAATAAGGATGATTTTCGCAAGACAGATTCGAG
TGGCCGTGGCTATCAACCGATGCTTTATGGTCTGAAAGAGGCACAAATTACGAAGGAAGAGTCATTGATTCATATCCATC
TGCAATTTGAAAAAGGACTGGAAAGGGAGTATGTGTATCGTGTGGAAGAAAAAGGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus mitis NCTC 12261

63.265

96.711

0.612
  comGF/cglF Streptococcus mitis SK321

60.811

97.368

0.592
  comGF/cglF Streptococcus pneumoniae Rx1

59.732

98.026

0.586
  comGF/cglF Streptococcus pneumoniae D39

59.732

98.026

0.586
  comGF/cglF Streptococcus pneumoniae R6

59.732

98.026

0.586
  comGF/cglF Streptococcus pneumoniae TIGR4

59.732

98.026

0.586
  comGF Lactococcus lactis subsp. cremoris KW2

46.377

90.789

0.421
  comYF Streptococcus mutans UA140

44.604

91.447

0.408
  comYF Streptococcus mutans UA159

43.885

91.447

0.401

Multiple sequence alignment