Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BER93_RS03900 Genome accession   NZ_CP016833
Coordinates   870066..870482 (+) Length   138 a.a.
NCBI ID   WP_002812272.1    Uniprot ID   A0A1Y6GZ11
Organism   Xanthomonas fragariae isolate Fap29     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 865066..875482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BER93_RS21855 (BER93_03880) - 865079..866204 (+) 1126 Protein_776 IS3 family transposase -
  BER93_RS03885 (BER93_03885) coaE 866964..867575 (-) 612 WP_002812280.1 dephospho-CoA kinase -
  BER93_RS03890 (BER93_03890) - 867589..868452 (-) 864 WP_002812278.1 A24 family peptidase -
  BER93_RS03895 (BER93_03895) pilC 868459..869715 (-) 1257 WP_040762949.1 type II secretion system F family protein Machinery gene
  BER93_RS03900 (BER93_03900) pilA 870066..870482 (+) 417 WP_002812272.1 pilin Machinery gene
  BER93_RS20080 - 870597..870995 (+) 399 WP_335673686.1 pilin -
  BER93_RS03905 (BER93_03905) pilB 871065..872801 (+) 1737 WP_002812268.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BER93_RS03915 (BER93_03915) - 873079..874446 (+) 1368 Protein_783 IS5 family transposase -
  BER93_RS03920 (BER93_03920) - 874517..875014 (-) 498 WP_231892748.1 Fic family protein -
  BER93_RS20085 - 875111..875293 (-) 183 WP_002812262.1 YhfG family protein -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14395.66 Da        Isoelectric Point: 8.4815

>NTDB_id=192545 BER93_RS03900 WP_002812272.1 870066..870482(+) (pilA) [Xanthomonas fragariae isolate Fap29]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQNYVAKSQVAAGLAEITPGKTQYEVMVNEGKASTITPDLIGLKTSTRCTI
STPSPSETGAITCALLNTSPKVAGQSISWTRAADGTWTCSTTLSATDKPKYAPATCQG

Nucleotide


Download         Length: 417 bp        

>NTDB_id=192545 BER93_RS03900 WP_002812272.1 870066..870482(+) (pilA) [Xanthomonas fragariae isolate Fap29]
ATGAAGAAGCAACAAGGCTTTACGTTGATTGAACTGATGATCGTGGTTGCCATCATTGCCATCCTGGCAGCAATTGCTCT
CCCTGCTTATCAGAATTATGTTGCCAAGTCGCAGGTCGCTGCAGGTCTGGCGGAAATCACTCCTGGTAAAACTCAGTATG
AAGTCATGGTCAACGAAGGTAAGGCTTCCACGATTACTCCTGATCTTATCGGTCTGAAGACGTCCACCCGCTGCACGATT
AGCACTCCGAGCCCGAGCGAGACTGGTGCAATCACCTGCGCTCTGCTGAACACAAGTCCTAAGGTTGCTGGTCAGAGCAT
CTCTTGGACGCGTGCTGCAGATGGTACGTGGACTTGCTCGACCACTTTGAGCGCCACTGACAAGCCCAAGTATGCACCGG
CAACCTGCCAGGGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-137)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y6GZ11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

50.35

100

0.522
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50

100

0.5
  pilA Pseudomonas aeruginosa PAK

43.151

100

0.457
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.588

98.551

0.449
  pilA Ralstonia pseudosolanacearum GMI1000

35.119

100

0.428
  comP Acinetobacter baylyi ADP1

38.411

100

0.42
  pilA Vibrio cholerae C6706

41.304

100

0.413
  pilA Vibrio cholerae strain A1552

41.304

100

0.413
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.304

100

0.413
  pilA Vibrio parahaemolyticus RIMD 2210633

41.667

95.652

0.399
  pilE Neisseria gonorrhoeae strain FA1090

34.615

100

0.391
  pilA2 Legionella pneumophila str. Paris

35.333

100

0.384
  pilA2 Legionella pneumophila strain ERS1305867

35.333

100

0.384

Multiple sequence alignment