Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PS273GM_RS05160 Genome accession   NZ_CP015641
Coordinates   1150556..1151701 (-) Length   381 a.a.
NCBI ID   WP_045423703.1    Uniprot ID   A0A172WMA3
Organism   Stutzerimonas stutzeri strain 273     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1145556..1156701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PS273GM_RS05135 (PS273GM_05135) - 1145633..1146649 (+) 1017 WP_046161612.1 aspartate carbamoyltransferase catalytic subunit -
  PS273GM_RS05140 (PS273GM_05140) - 1146649..1147920 (+) 1272 WP_064480825.1 dihydroorotase -
  PS273GM_RS05145 (PS273GM_05145) - 1147975..1148379 (-) 405 WP_064480826.1 NINE protein -
  PS273GM_RS05150 (PS273GM_05150) - 1148514..1149725 (+) 1212 WP_064480827.1 putative DNA modification/repair radical SAM protein -
  PS273GM_RS05155 (PS273GM_05155) - 1149725..1150555 (+) 831 WP_064480828.1 TIGR03915 family putative DNA repair protein -
  PS273GM_RS05160 (PS273GM_05160) pilU 1150556..1151701 (-) 1146 WP_045423703.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PS273GM_RS05165 (PS273GM_05165) pilT 1151777..1152811 (-) 1035 WP_045423701.1 type IV pilus twitching motility protein PilT Machinery gene
  PS273GM_RS05170 (PS273GM_05170) - 1152878..1153591 (+) 714 WP_064480829.1 YggS family pyridoxal phosphate-dependent enzyme -
  PS273GM_RS05175 (PS273GM_05175) proC 1153605..1154432 (+) 828 WP_064480830.1 pyrroline-5-carboxylate reductase -
  PS273GM_RS05180 (PS273GM_05180) - 1154442..1155032 (+) 591 WP_045423695.1 YggT family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42409.61 Da        Isoelectric Point: 6.5057

>NTDB_id=181644 PS273GM_RS05160 WP_045423703.1 1150556..1151701(-) (pilU) [Stutzerimonas stutzeri strain 273]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKILPVNKTPMSPEMTRETVHAVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLEDLKLPEVLKKLAMTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTDSFDVALKNTLRQAPDVILIGEVRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTDLGMQTFDQA
LYSLYVQGEITYEDALAHADSSNDLRLMIKLGSETDGEHLTSVSQGLTLEVSDDDTGRSFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=181644 PS273GM_RS05160 WP_045423703.1 1150556..1151701(-) (pilU) [Stutzerimonas stutzeri strain 273]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTGGAAAAAGGCGGTTCCGACCTGTTCATTACCGCTGGCGTGCCGCCGTC
AATGAAGGTCAACGGCAAGATCCTGCCAGTCAATAAGACGCCGATGTCACCGGAAATGACCCGCGAAACGGTTCACGCGG
TGATGAACGAGCAGCAGCGTCGCGAGTTCACGGAAAACCACGAATGCAACTTCGCCATCAGCGCGCGCGGCATTGGTCGT
TTTCGGGTCAGCGCCTTCTATCAGCGCAACCTCGCGGGCATGGTACTGCGCCGTATCGAGACCAACATACCAACGCTGGA
AGACCTGAAGCTTCCCGAAGTGTTGAAAAAGCTGGCAATGACCAAGCGGGGGCTGGTGCTGTTCGTCGGTGCGACCGGTA
CCGGCAAATCGACCTCGTTGGCTGCGATGATCGGCTACCGCAACAAGAACTCCAGCGGCCATATCATCTCGATCGAAGAT
CCGATTGAGTTCATTCACCAGCATCAGAGCTGCATCGTCACCCAGCGTGAAGTCGGAATCGATACGGACTCCTTCGATGT
AGCGCTGAAAAACACCCTGCGCCAGGCGCCGGATGTGATCCTGATCGGTGAAGTGCGGACACGGGAAACCATGGACTACG
CCGTTGCATTCGCCGAGACCGGCCACCTTTGTCTGGCGACCCTGCACGCCAACAACGCTAACCAGGCGCTGGACCGAATC
ATCAACTTCTTCCCGGCTGATCGGCAGCAGCAGGTCTGGATGGATCTGTCGCTGAACCTCAAAGCCATCGTCGCTCAGCA
GCTGATCCCGACGCCCGATGGCAAAGGCCGTCGCGCGGTAATCGAAGTGCTGATCAATACTCCCCTGGCGGCTGATCTGA
TCCGTAAAGGAGAGGTTCACGAGCTCAAATCGCTGATGAAACGCTCCACCGACCTAGGCATGCAGACCTTCGACCAGGCG
CTGTACAGCCTCTACGTGCAAGGCGAAATCACTTACGAAGACGCCCTGGCGCATGCCGACTCCTCCAACGATCTACGGCT
GATGATCAAGCTTGGTTCGGAAACCGACGGTGAACATCTCACCAGCGTATCTCAGGGGCTTACTCTGGAAGTCAGCGACG
ACGATACGGGTCGCAGCTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172WMA3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

96.325

100

0.963
  pilU Acinetobacter baylyi ADP1

66.106

93.701

0.619
  pilU Vibrio cholerae strain A1552

59.429

91.864

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

88.976

0.391
  pilT Neisseria meningitidis 8013

41.159

90.551

0.373
  pilT Neisseria gonorrhoeae MS11

41.159

90.551

0.373
  pilT Legionella pneumophila strain Lp02

42.09

87.927

0.37
  pilT Legionella pneumophila strain ERS1305867

42.09

87.927

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.716

88.714

0.37
  pilT Vibrio cholerae strain A1552

41.716

88.714

0.37
  pilT Pseudomonas aeruginosa PAK

41.791

87.927

0.367
  pilT Pseudomonas stutzeri DSM 10701

41.493

87.927

0.365
  pilT Acinetobacter baylyi ADP1

40.469

89.501

0.362

Multiple sequence alignment