Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   A3K93_RS11055 Genome accession   NZ_CP015594
Coordinates   2288090..2288521 (+) Length   143 a.a.
NCBI ID   WP_067731267.1    Uniprot ID   -
Organism   Acinetobacter sp. NCu2D-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2283819..2301098 2288090..2288521 within 0
IScluster/Tn 2276952..2289612 2288090..2288521 within 0


Gene organization within MGE regions


Location: 2276952..2301098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3K93_RS14740 - 2276952..2278084 (+) 1133 WP_227509860.1 IS3 family transposase -
  A3K93_RS11005 (A3K93_11005) tfpZ 2278116..2278817 (-) 702 WP_067731263.1 TfpX/TfpZ family type IV pilin accessory protein -
  A3K93_RS11015 (A3K93_11015) - 2278996..2280071 (+) 1076 WP_101494812.1 IS3 family transposase -
  A3K93_RS11020 (A3K93_11020) - 2280081..2280647 (-) 567 WP_067731264.1 pilin -
  A3K93_RS11025 (A3K93_11025) - 2280929..2282830 (+) 1902 WP_067731265.1 acyltransferase family protein -
  A3K93_RS11030 (A3K93_11030) - 2283029..2283412 (+) 384 WP_001055589.1 transposase -
  A3K93_RS11035 (A3K93_11035) tnpB 2283409..2283744 (+) 336 WP_067731266.1 IS66 family insertion sequence element accessory protein TnpB -
  A3K93_RS11040 (A3K93_11040) - 2283819..2285219 (+) 1401 Protein_2125 IS66 family transposase -
  A3K93_RS11045 (A3K93_11045) ltrA 2285795..2287456 (+) 1662 WP_067731626.1 group II intron reverse transcriptase/maturase -
  A3K93_RS11050 (A3K93_11050) - 2287561..2287788 (+) 228 Protein_2127 transposase domain-containing protein -
  A3K93_RS11055 (A3K93_11055) pilA2 2288090..2288521 (+) 432 WP_067731267.1 pilin Machinery gene
  A3K93_RS11060 (A3K93_11060) - 2288680..2289612 (-) 933 WP_081408534.1 IS5-like element IS17 family transposase -
  A3K93_RS11065 (A3K93_11065) - 2289684..2290043 (+) 360 WP_067731268.1 hypothetical protein -
  A3K93_RS11070 (A3K93_11070) - 2290216..2291352 (+) 1137 WP_067731269.1 glycosyltransferase family 4 protein -
  A3K93_RS11075 (A3K93_11075) - 2291337..2292443 (+) 1107 WP_157883276.1 glycosyltransferase family 4 protein -
  A3K93_RS11080 (A3K93_11080) - 2292424..2293710 (-) 1287 WP_067731271.1 CDP-glycerol glycerophosphotransferase family protein -
  A3K93_RS11085 (A3K93_11085) ispD 2293697..2295061 (-) 1365 WP_067731272.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  A3K93_RS11090 (A3K93_11090) - 2295058..2295969 (-) 912 WP_067731273.1 LicD family protein -
  A3K93_RS11095 (A3K93_11095) - 2295959..2296579 (-) 621 WP_067731274.1 class I SAM-dependent methyltransferase -
  A3K93_RS11100 (A3K93_11100) - 2296725..2297687 (+) 963 WP_067729673.1 IS30 family transposase -
  A3K93_RS11105 (A3K93_11105) - 2297663..2298376 (-) 714 WP_067731275.1 aspartate/glutamate racemase family protein -
  A3K93_RS11110 (A3K93_11110) - 2298386..2299378 (-) 993 WP_067731276.1 adenylyltransferase/cytidyltransferase family protein -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14756.94 Da        Isoelectric Point: 10.0715

>NTDB_id=181252 A3K93_RS11055 WP_067731267.1 2288090..2288521(+) (pilA2) [Acinetobacter sp. NCu2D-2]
MNTMQKGFTLIELMIVVAIIGILAAIAIPAYQNYTKRSHVSEGLSLAGGAKTGITEFYSSNNRWPGATGNASVGLAPAGS
IKGNAVRSVLVNGSTIVITYNTKVAAGETLIMKGTPSGGGMEWSCSGSTSGTLDPKFRPSNCR

Nucleotide


Download         Length: 432 bp        

>NTDB_id=181252 A3K93_RS11055 WP_067731267.1 2288090..2288521(+) (pilA2) [Acinetobacter sp. NCu2D-2]
ATGAATACAATGCAGAAGGGCTTCACGCTCATCGAGCTTATGATTGTGGTCGCTATTATCGGTATTCTTGCTGCAATTGC
GATTCCTGCTTATCAGAACTATACGAAACGTTCACACGTTTCTGAAGGTTTAAGTTTGGCTGGTGGTGCTAAAACAGGTA
TTACTGAGTTTTATTCATCTAATAATCGTTGGCCTGGCGCTACCGGTAATGCTTCAGTTGGCTTGGCACCTGCTGGCAGT
ATTAAGGGTAATGCTGTAAGATCTGTGCTTGTAAATGGTAGTACGATTGTTATTACTTATAATACTAAAGTTGCTGCAGG
CGAAACTTTAATTATGAAAGGTACTCCATCAGGTGGTGGTATGGAGTGGTCTTGTTCCGGTTCAACTAGTGGTACTTTAG
ATCCTAAATTCCGTCCTTCAAATTGTCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

51.429

97.902

0.504
  pilA Ralstonia pseudosolanacearum GMI1000

42.424

100

0.49
  pilA2 Legionella pneumophila strain ERS1305867

49.286

97.902

0.483
  pilE Neisseria gonorrhoeae MS11

41.358

100

0.469
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.638

100

0.469
  pilE Neisseria gonorrhoeae strain FA1090

41.25

100

0.462
  comP Acinetobacter baylyi ADP1

40.94

100

0.427
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.162

100

0.385
  pilA/pilA1 Eikenella corrodens VA1

36.184

100

0.385
  pilA Haemophilus influenzae Rd KW20

37.762

100

0.378
  pilA/pilAII Pseudomonas stutzeri DSM 10701

35.811

100

0.371
  pilA Haemophilus influenzae 86-028NP

36.364

100

0.364
  pilA Vibrio parahaemolyticus RIMD 2210633

41.935

86.713

0.364

Multiple sequence alignment