Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AC731_RS12785 Genome accession   NZ_CP014646
Coordinates   2725153..2726286 (+) Length   377 a.a.
NCBI ID   WP_048706607.1    Uniprot ID   A0A127K6Z7
Organism   Thauera humireducens strain SgZ-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2720153..2731286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC731_RS12760 (AC731_012760) - 2720393..2721673 (+) 1281 WP_048706605.1 dihydroorotase -
  AC731_RS12765 (AC731_012765) - 2721776..2722357 (-) 582 WP_004292187.1 YggT family protein -
  AC731_RS12770 (AC731_012770) proC 2722364..2723188 (-) 825 WP_048706606.1 pyrroline-5-carboxylate reductase -
  AC731_RS12775 (AC731_012775) - 2723235..2723936 (-) 702 WP_038012414.1 YggS family pyridoxal phosphate-dependent enzyme -
  AC731_RS12780 (AC731_012780) pilT 2724028..2725071 (+) 1044 WP_038012416.1 type IV pilus twitching motility protein PilT Machinery gene
  AC731_RS12785 (AC731_012785) pilU 2725153..2726286 (+) 1134 WP_048706607.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AC731_RS12790 (AC731_012790) - 2726340..2727509 (-) 1170 WP_048706608.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  AC731_RS12795 (AC731_012795) - 2727673..2728695 (-) 1023 WP_048706611.1 formate dehydrogenase subunit gamma -
  AC731_RS12800 (AC731_012800) - 2728706..2728942 (-) 237 WP_004292193.1 hypothetical protein -
  AC731_RS12805 (AC731_012805) fdh3B 2728939..2729571 (-) 633 WP_004292194.1 formate dehydrogenase FDH3 subunit beta -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42151.42 Da        Isoelectric Point: 6.5064

>NTDB_id=172974 AC731_RS12785 WP_048706607.1 2725153..2726286(+) (pilU) [Thauera humireducens strain SgZ-1]
MERDQALKFMHDLLRLMLQKNGSDLFITAGFPPAVKVDGRITPQSNQALQPQHTAELARAIMNDRQAAEFEASKECNFGI
SPAGIGRFRANAFVQQGKVGLVLRTIPQRIPQFDELGLPPVLTDIAMSKRGLVIFVGGTGTGKTTSLASMVDYRNENSYG
HIITVEDPIEFVHAHKNCIVSQREIGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEERRQQLLMDLSLNLRAMVSQRLLPLKDRKGRVPAVEVMLNSPLISDLIFKGNVPEIKEIMKRSRELG
MQTFDQALFDLYEAQKISYEDALRNADSINDLRLQIKLHSKLGERDLSSGTENLGIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=172974 AC731_RS12785 WP_048706607.1 2725153..2726286(+) (pilU) [Thauera humireducens strain SgZ-1]
ATGGAACGCGACCAGGCACTCAAGTTCATGCACGACCTGCTGCGGCTGATGCTGCAGAAGAACGGCTCCGACCTCTTCAT
CACCGCGGGCTTTCCGCCGGCCGTGAAAGTGGACGGCCGCATCACACCGCAGTCCAACCAGGCGCTGCAACCGCAGCACA
CGGCCGAACTGGCCCGCGCGATCATGAACGACCGCCAGGCTGCTGAATTCGAAGCCAGCAAGGAATGCAACTTCGGCATC
TCGCCGGCCGGCATCGGCCGCTTCCGCGCCAATGCCTTCGTGCAGCAGGGCAAAGTCGGGCTGGTGTTGCGGACCATCCC
GCAGCGCATCCCGCAATTCGATGAGCTCGGCCTGCCGCCCGTGCTGACCGACATCGCGATGAGCAAGCGCGGGCTGGTGA
TCTTCGTCGGCGGCACCGGCACCGGCAAGACGACCTCGCTGGCGTCGATGGTCGACTACCGCAACGAGAACAGCTACGGC
CACATCATCACGGTCGAGGATCCGATCGAGTTCGTGCATGCGCACAAGAACTGCATCGTTTCGCAGCGCGAGATCGGCAT
CGACACCGACTCGTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACGATGGACTACGCGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTCGCGACACTGCACGCCAACAGCGCC
AACCAGGCCATCGACCGCATCATCAACTTCTTCCCCGAGGAACGCCGCCAGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCGCCATGGTCTCGCAGCGCCTGCTGCCACTGAAGGACCGCAAGGGCCGGGTGCCGGCCGTCGAGGTGATGCTCAATT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCAACGTGCCCGAGATCAAGGAGATCATGAAGCGCTCGCGCGAACTGGGC
ATGCAGACCTTCGACCAAGCGCTGTTCGACCTCTACGAGGCGCAGAAGATCTCGTACGAGGACGCGCTGCGCAACGCCGA
CTCGATCAACGACCTGCGCCTTCAGATCAAGCTGCACAGCAAGCTGGGCGAGCGCGATCTCAGCAGCGGCACCGAGAACC
TCGGCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127K6Z7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.431

96.021

0.599
  pilU Acinetobacter baylyi ADP1

60.632

92.308

0.56
  pilU Vibrio cholerae strain A1552

55.493

94.164

0.523
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.075

88.859

0.401
  pilT Legionella pneumophila strain Lp02

43.807

87.798

0.385
  pilT Legionella pneumophila strain ERS1305867

43.807

87.798

0.385
  pilT Acinetobacter nosocomialis M2

42.598

87.798

0.374
  pilT Acinetobacter baumannii D1279779

42.598

87.798

0.374
  pilT Acinetobacter baumannii strain A118

42.598

87.798

0.374
  pilT Pseudomonas aeruginosa PAK

41.791

88.859

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.791

88.859

0.371
  pilT Acinetobacter baylyi ADP1

43.438

84.881

0.369

Multiple sequence alignment