Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AC731_RS12780 Genome accession   NZ_CP014646
Coordinates   2724028..2725071 (+) Length   347 a.a.
NCBI ID   WP_038012416.1    Uniprot ID   A0A127K7T8
Organism   Thauera humireducens strain SgZ-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2719028..2730071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC731_RS12755 (AC731_012755) - 2719401..2720360 (+) 960 WP_004292185.1 aspartate carbamoyltransferase catalytic subunit -
  AC731_RS12760 (AC731_012760) - 2720393..2721673 (+) 1281 WP_048706605.1 dihydroorotase -
  AC731_RS12765 (AC731_012765) - 2721776..2722357 (-) 582 WP_004292187.1 YggT family protein -
  AC731_RS12770 (AC731_012770) proC 2722364..2723188 (-) 825 WP_048706606.1 pyrroline-5-carboxylate reductase -
  AC731_RS12775 (AC731_012775) - 2723235..2723936 (-) 702 WP_038012414.1 YggS family pyridoxal phosphate-dependent enzyme -
  AC731_RS12780 (AC731_012780) pilT 2724028..2725071 (+) 1044 WP_038012416.1 type IV pilus twitching motility protein PilT Machinery gene
  AC731_RS12785 (AC731_012785) pilU 2725153..2726286 (+) 1134 WP_048706607.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AC731_RS12790 (AC731_012790) - 2726340..2727509 (-) 1170 WP_048706608.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  AC731_RS12795 (AC731_012795) - 2727673..2728695 (-) 1023 WP_048706611.1 formate dehydrogenase subunit gamma -
  AC731_RS12800 (AC731_012800) - 2728706..2728942 (-) 237 WP_004292193.1 hypothetical protein -
  AC731_RS12805 (AC731_012805) fdh3B 2728939..2729571 (-) 633 WP_004292194.1 formate dehydrogenase FDH3 subunit beta -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38587.34 Da        Isoelectric Point: 7.0159

>NTDB_id=172973 AC731_RS12780 WP_038012416.1 2724028..2725071(+) (pilT) [Thauera humireducens strain SgZ-1]
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEHKEVHAMVYDIMNDGQRKQFEETWECDFSFAVPNLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEELNCPKIFKEIANQPRGIVLVTGPTGSGKSTTLAAMIDYVNENEYGHILTVED
PIEFVHESKRCLINQREVHRDTMSFNNALRASLREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGAGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQNVGMQTLDQC
LADLVRRNVVSAAEAKLRAQNKDNFAV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=172973 AC731_RS12780 WP_038012416.1 2724028..2725071(+) (pilT) [Thauera humireducens strain SgZ-1]
ATGGACATCACCGAACTCCTCGCCTTCGCCGTCAAGAACAAGGCTTCCGACCTTCACCTTTCGGCGGGCCTGCCGCCCAT
GATCCGTGTGCATGGCGACGTGCGCCGCATCAACCTGCCGCCCATGGAGCACAAGGAAGTGCATGCCATGGTGTACGACA
TCATGAACGACGGGCAGCGCAAGCAGTTCGAGGAAACCTGGGAGTGCGATTTCTCGTTCGCGGTTCCCAACCTCGCCCGC
TTCCGTGTGAACGCGTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCGTCCAAGGTGCTGACGCTCGA
AGAGCTGAACTGCCCGAAGATCTTCAAGGAGATCGCCAACCAGCCGCGCGGCATCGTGCTGGTCACCGGCCCGACCGGCT
CCGGCAAATCGACCACGCTCGCGGCGATGATCGACTACGTCAACGAGAACGAGTACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCATGAATCCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCATGTCCTTCAACAA
CGCGCTGCGCGCCTCCTTGCGCGAGGACCCGGACGTGATCCTGGTGGGCGAAATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTCGTGTTCGGCACGCTGCACACCTCGTCCGCCGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCTGCCGCGGAGAAGGACATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGAC
CCTGCTCAAGACCAAGGACGGTGCGGGGCGCGTGGCCGCGCACGAGATCATGATCGGCACCCCCGCCATCCGCAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTATTCGGCGATCCAGACCGGCCAGAATGTCGGCATGCAGACGCTGGACCAGTGC
CTCGCCGACCTCGTGCGCCGCAACGTGGTCTCGGCCGCCGAGGCGAAGCTCCGCGCGCAGAACAAGGACAACTTCGCCGT
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127K7T8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.13

99.424

0.787
  pilT Acinetobacter baumannii D1279779

79.13

99.424

0.787
  pilT Acinetobacter baumannii strain A118

79.13

99.424

0.787
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Acinetobacter baylyi ADP1

76.232

99.424

0.758
  pilT Legionella pneumophila strain Lp02

73.043

99.424

0.726
  pilT Legionella pneumophila strain ERS1305867

73.043

99.424

0.726
  pilT Neisseria meningitidis 8013

70.809

99.712

0.706
  pilT Neisseria gonorrhoeae MS11

70.52

99.712

0.703
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.048

96.83

0.669
  pilT Vibrio cholerae strain A1552

69.048

96.83

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.179

99.712

0.53
  pilU Acinetobacter baylyi ADP1

42.529

100

0.427
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.739

99.424

0.415

Multiple sequence alignment