Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BMR72_RS25375 Genome accession   NZ_CP017969
Coordinates   5257003..5257467 (-) Length   154 a.a.
NCBI ID   WP_031630343.1    Uniprot ID   Q93PL5
Organism   Pseudomonas aeruginosa isolate B10W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5252003..5262467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMR72_RS25355 (BMR72_24485) ampD 5252694..5253260 (-) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BMR72_RS25360 (BMR72_24490) - 5253411..5255666 (-) 2256 WP_003134886.1 DUF1631 domain-containing protein -
  BMR72_RS25365 (BMR72_24495) nadC 5255952..5256800 (+) 849 WP_003125200.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BMR72_RS25375 pilA 5257003..5257467 (-) 465 WP_031630343.1 pilin Machinery gene
  BMR72_RS25380 (BMR72_24505) pilB 5257697..5259397 (+) 1701 WP_003094647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BMR72_RS25385 (BMR72_24510) pilC 5259401..5260621 (+) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  BMR72_RS25390 (BMR72_24515) pilD 5260625..5261497 (+) 873 WP_003134888.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  BMR72_RS25395 (BMR72_24520) coaE 5261494..5262105 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  BMR72_RS25400 (BMR72_24525) yacG 5262102..5262302 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15780.91 Da        Isoelectric Point: 6.1571

>NTDB_id=172267 BMR72_RS25375 WP_031630343.1 5257003..5257467(-) (pilA) [Pseudomonas aeruginosa isolate B10W]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVSRSQVSRVMAEAGSLKTAVEACLQDGRTAVGTAAGQCDPGATGSSL
LTGASQTSQTLPTNTGVPQVLDPLTTQTTIIATFGNGASAAISGQTLTWTRDVNGGWSCATTVDAKFRPNGCTD

Nucleotide


Download         Length: 465 bp        

>NTDB_id=172267 BMR72_RS25375 WP_031630343.1 5257003..5257467(-) (pilA) [Pseudomonas aeruginosa isolate B10W]
ATGAAAGCACAGAAGGGTTTTACTCTGATTGAACTGATGATCGTAGTTGCGATCATTGGTATTCTGGCTGCAATCGCAAT
TCCCCAATATCAGAACTATGTTTCTCGCTCTCAGGTCTCCAGGGTTATGGCAGAGGCTGGCTCCTTGAAGACTGCAGTTG
AGGCCTGCCTCCAGGATGGTCGTACTGCTGTGGGTACTGCTGCTGGTCAATGCGACCCGGGTGCGACGGGTTCCAGTTTG
TTGACTGGTGCTTCTCAGACTTCTCAAACCCTGCCAACCAATACCGGTGTTCCGCAGGTTCTGGATCCTCTGACTACTCA
AACCACTATCATTGCGACTTTTGGTAACGGTGCATCCGCAGCTATTTCTGGCCAGACTCTGACCTGGACTCGTGATGTTA
ATGGTGGCTGGAGCTGTGCTACTACCGTAGATGCTAAATTCCGTCCTAATGGCTGTACTGACTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q93PL5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

46.753

100

0.468
  pilA Vibrio cholerae strain A1552

43.59

100

0.442
  pilA Vibrio cholerae C6706

43.59

100

0.442
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.59

100

0.442
  comP Acinetobacter baylyi ADP1

40.12

100

0.435
  pilA Vibrio parahaemolyticus RIMD 2210633

40.881

100

0.422
  pilA Acinetobacter baumannii strain A118

41.83

99.351

0.416
  pilA2 Legionella pneumophila strain ERS1305867

41.558

100

0.416
  pilA2 Legionella pneumophila str. Paris

40.909

100

0.409
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40

100

0.403
  pilE Neisseria gonorrhoeae MS11

33.908

100

0.383
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.411

98.052

0.377

Multiple sequence alignment