Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AL552_RS13100 Genome accession   NZ_CP014094
Coordinates   2477013..2477462 (+) Length   149 a.a.
NCBI ID   WP_104969781.1    Uniprot ID   A0AAX1XLS0
Organism   Vibrio diabolicus strain FDAARGOS_96     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2472013..2482462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL552_RS13085 (AL552_21240) pdhR 2474041..2474808 (-) 768 WP_005396680.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AL552_RS13090 (AL552_21245) ampD 2475214..2475765 (-) 552 WP_005396682.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AL552_RS13095 (AL552_21250) nadC 2475858..2476745 (+) 888 WP_104969780.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AL552_RS13100 (AL552_21255) pilA 2477013..2477462 (+) 450 WP_104969781.1 pilin Machinery gene
  AL552_RS13105 (AL552_21260) pilB 2477466..2479151 (+) 1686 WP_104969782.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AL552_RS13110 (AL552_21265) pilC 2479176..2480399 (+) 1224 WP_104969783.1 type II secretion system F family protein Machinery gene
  AL552_RS13115 (AL552_21270) pilD 2480464..2481333 (+) 870 WP_012841418.1 A24 family peptidase Machinery gene
  AL552_RS13120 (AL552_21275) coaE 2481334..2481948 (+) 615 WP_047008979.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15583.63 Da        Isoelectric Point: 4.7246

>NTDB_id=168326 AL552_RS13100 WP_104969781.1 2477013..2477462(+) (pilA) [Vibrio diabolicus strain FDAARGOS_96]
MKNSKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVAKSELGAGLATITSVRTNVEDYMVTNGSFPDGSTSGQTEAE
LGVIDPNNGTITFDSTNGNILLTFASTGNSPDINGAKIALNRATGGTWTCKATPAPELLPKSCTQDTSL

Nucleotide


Download         Length: 450 bp        

>NTDB_id=168326 AL552_RS13100 WP_104969781.1 2477013..2477462(+) (pilA) [Vibrio diabolicus strain FDAARGOS_96]
ATGAAAAACAGTAAACAGAAAAAACAACAGGGTTTTACTCTGATCGAATTAATGATTGTGGTGGCAGTAATTGGTGTGCT
AGCAGCGATTGCAATACCACAATACCAGAACTACGTAGCTAAGTCTGAGCTAGGCGCTGGGTTAGCAACAATTACCTCTG
TGAGAACAAATGTTGAGGATTATATGGTAACAAACGGATCTTTTCCTGATGGTAGTACTTCCGGACAAACAGAAGCGGAG
TTGGGTGTAATTGACCCAAACAATGGAACTATTACATTTGACTCAACCAATGGAAATATACTACTTACTTTTGCTTCTAC
CGGAAATAGCCCTGACATCAACGGTGCTAAAATTGCTCTTAACAGAGCTACTGGAGGTACATGGACATGTAAAGCAACCC
CTGCACCTGAGCTTCTCCCTAAATCATGTACCCAAGATACTAGCTTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

50.649

100

0.523
  pilA Vibrio cholerae strain A1552

50.649

100

0.523
  pilA Vibrio cholerae C6706

50.649

100

0.523
  pilA Vibrio parahaemolyticus RIMD 2210633

45.89

97.987

0.45
  pilA Pseudomonas aeruginosa PAK

44.295

100

0.443
  pilA Acinetobacter baumannii strain A118

43.885

93.289

0.409
  pilA Acinetobacter nosocomialis M2

39.31

97.315

0.383
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.437

95.302

0.376
  pilA/pilA1 Eikenella corrodens VA1

33.951

100

0.369
  pilA2 Legionella pneumophila strain ERS1305867

38.028

95.302

0.362
  pilA2 Legionella pneumophila str. Paris

37.241

97.315

0.362

Multiple sequence alignment