Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WL1483_RS10140 Genome accession   NZ_CP013067
Coordinates   2049189..2050310 (-) Length   373 a.a.
NCBI ID   WP_050664732.1    Uniprot ID   -
Organism   Aeromonas schubertii strain WL1483     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2044189..2055310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WL1483_RS10110 (WL1483_144) - 2044478..2045404 (-) 927 WP_060585930.1 DUF2157 domain-containing protein -
  WL1483_RS10115 (WL1483_4136) - 2045457..2045684 (-) 228 Protein_1965 TMEM165/GDT1 family protein -
  WL1483_RS10120 (WL1483_1101) - 2045960..2046289 (-) 330 WP_060585932.1 translation initiation factor -
  WL1483_RS10125 (WL1483_621) - 2046407..2046634 (+) 228 WP_050664729.1 FeoA family protein -
  WL1483_RS10130 (WL1483_3220) feoB 2046631..2048898 (+) 2268 WP_060585937.1 Fe(2+) transporter permease subunit FeoB -
  WL1483_RS10135 (WL1483_2612) - 2048895..2049137 (+) 243 WP_050664731.1 FeoC-like transcriptional regulator -
  WL1483_RS10140 pilU 2049189..2050310 (-) 1122 WP_050664732.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WL1483_RS10145 (WL1483_1225) - 2050366..2050719 (-) 354 WP_060585938.1 DMT family protein -
  WL1483_RS10150 (WL1483_3709) - 2050819..2051553 (+) 735 WP_060585939.1 substrate-binding periplasmic protein -
  WL1483_RS10155 (WL1483_3036) btuC 2051604..2052560 (+) 957 WP_235513100.1 vitamin B12 ABC transporter permease BtuC -
  WL1483_RS10160 (WL1483_12) - 2052547..2053281 (+) 735 WP_060585942.1 ATP-binding cassette domain-containing protein -
  WL1483_RS10165 (WL1483_2166) - 2053284..2054348 (-) 1065 WP_060585944.1 3-deoxy-7-phosphoheptulonate synthase -
  WL1483_RS10170 (WL1483_3957) - 2054493..2054873 (+) 381 WP_050664738.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41574.62 Da        Isoelectric Point: 6.7299

>NTDB_id=159536 WL1483_RS10140 WP_050664732.1 2049189..2050310(-) (pilU) [Aeromonas schubertii strain WL1483]
MELQPLLQILAHQDGSDLYLSTGAPPSAKFQGVLKPLGSDPLPPGETARIADSIMDEEQRIAFAKDLEMNLALSIPKVGR
FRVNIFKQRNEISIVARNIKTEIPRFEDLKLPDVLLETIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEYVHQHKRCIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLAISTLHANNANQALDRI
INFFPEERRPQLLQDLGNNLKAFISQRLVKTKEGGRRAAVEVMLGTHTVRDMVKRSEFGGLKEVMEKSRALGMITFDQAL
FELVVEGCIEEEEAVKNADSANNLRLKIKLWREKGQIANSNDSSGWAIDMGDH

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=159536 WL1483_RS10140 WP_050664732.1 2049189..2050310(-) (pilU) [Aeromonas schubertii strain WL1483]
ATGGAATTGCAACCCCTGCTGCAGATCCTGGCCCACCAGGATGGCTCTGATCTCTATCTCTCTACCGGTGCCCCCCCCAG
CGCCAAGTTTCAGGGGGTGCTCAAACCCCTGGGCAGCGATCCCCTCCCCCCCGGTGAGACGGCCCGCATCGCCGACAGCA
TCATGGACGAAGAGCAACGCATCGCCTTCGCCAAGGATCTGGAGATGAACCTGGCCCTCTCCATCCCCAAGGTGGGGCGC
TTTCGTGTCAATATCTTCAAACAGCGCAACGAGATCTCCATCGTCGCCCGTAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTCAAGCTGCCCGACGTGCTGCTCGAGACCATCATGGAGAAGCGGGGTCTGGTGCTGTTTGTCGGCGGTACCGGCT
CGGGCAAATCCACCTCGCTGGCGGCCCTCATCGACCACCGTAACCGTAACAGCGGCGGCCACATCATCACCATCGAGGAC
CCTGTCGAGTATGTCCACCAGCACAAACGCTGTATCATCAACCAGCGCGAGGTGGGGGTCGACACCCGCAGCTTCCACGC
AGCCCTCAAAAATACCCTGCGCCAGGCACCGGATGTGATCCTTATCGGGGAGATCCGCGATCGGGAGACCATGGAGCACG
CTCTCGCCTTTGCCGAAACCGGGCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCCCTCGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGTCCCCAGCTGCTGCAAGATCTGGGCAACAACCTCAAGGCCTTCATCTCCCAGCG
GCTGGTCAAGACCAAGGAGGGGGGGCGCCGCGCCGCCGTCGAGGTGATGCTCGGCACCCACACGGTACGCGACATGGTCA
AACGCAGCGAATTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAGGGCCCTTGGCATGATCACCTTCGATCAGGCCCTC
TTCGAACTGGTGGTCGAGGGGTGCATCGAAGAAGAAGAGGCAGTGAAAAATGCCGATTCGGCCAACAACTTGCGCCTCAA
GATCAAGCTCTGGCGTGAGAAGGGACAAATCGCCAACAGCAACGACTCCTCCGGATGGGCCATCGACATGGGCGACCACT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.96

100

0.55
  pilU Acinetobacter baylyi ADP1

53.521

95.174

0.509
  pilU Vibrio cholerae strain A1552

49.596

99.464

0.493
  pilT Legionella pneumophila strain Lp02

41.617

89.544

0.373
  pilT Legionella pneumophila strain ERS1305867

41.617

89.544

0.373
  pilT Pseudomonas aeruginosa PAK

40.116

92.225

0.37
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.413

90.885

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.535

92.225

0.365
  pilT Vibrio cholerae strain A1552

40.541

89.276

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.541

89.276

0.362
  pilT Acinetobacter baumannii D1279779

40.419

89.544

0.362
  pilT Acinetobacter baumannii strain A118

40.419

89.544

0.362
  pilT Acinetobacter nosocomialis M2

40.419

89.544

0.362

Multiple sequence alignment