Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AC610_RS17215 Genome accession   NZ_CP012063
Coordinates   3764884..3765324 (-) Length   146 a.a.
NCBI ID   WP_127446519.1    Uniprot ID   A0A3T0G1A3
Organism   Xanthomonas phaseoli pv. phaseoli strain ISO18C8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 3765618..3772152 3764884..3765324 flank 294


Gene organization within MGE regions


Location: 3764884..3772152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC610_RS17215 (AC610_16405) pilA 3764884..3765324 (-) 441 WP_127446519.1 pilin Machinery gene
  AC610_RS17220 (AC610_16410) - 3765618..3766844 (-) 1227 WP_011345585.1 IS256-like element ISXax1 family transposase -
  AC610_RS17225 (AC610_16415) pilC 3767016..3768272 (+) 1257 WP_039573972.1 type II secretion system F family protein Machinery gene
  AC610_RS17230 (AC610_16420) - 3768279..3769142 (+) 864 WP_039573976.1 A24 family peptidase -
  AC610_RS17235 (AC610_16425) coaE 3769153..3769764 (+) 612 WP_007962470.1 dephospho-CoA kinase -
  AC610_RS24745 - 3769946..3771016 (+) 1071 WP_157926032.1 RHS repeat domain-containing protein -
  AC610_RS17245 (AC610_16435) - 3771065..3772152 (+) 1088 WP_099770620.1 IS3-like element ISXac2 family transposase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15219.42 Da        Isoelectric Point: 7.6336

>NTDB_id=150972 AC610_RS17215 WP_127446519.1 3764884..3765324(-) (pilA) [Xanthomonas phaseoli pv. phaseoli strain ISO18C8]
MKKQHGFTLIELMIVIAIIAILAAIALPQYQNYVAKSQVTAGLAELSPGKTQYETLLNEGKGSTLTTADAVGLQASSTTN
RCSTITVLAPDATTGAQTNALSCTLAGSPRIVGKKITWARDTNGVWTCSTDIATGDEAKFAPATCQ

Nucleotide


Download         Length: 441 bp        

>NTDB_id=150972 AC610_RS17215 WP_127446519.1 3764884..3765324(-) (pilA) [Xanthomonas phaseoli pv. phaseoli strain ISO18C8]
ATGAAGAAGCAACACGGTTTCACTCTGATCGAACTCATGATCGTCATCGCGATCATTGCAATCTTGGCCGCCATTGCACT
GCCGCAGTACCAGAACTATGTCGCAAAGTCGCAGGTCACTGCTGGTCTGGCCGAGCTGAGCCCGGGCAAGACGCAGTACG
AAACTCTTTTGAACGAAGGCAAGGGCTCGACGCTGACGACTGCTGATGCAGTTGGTCTGCAGGCCTCGTCGACCACCAAT
CGCTGTTCGACGATTACGGTGTTGGCTCCGGATGCTACAACCGGTGCGCAGACTAACGCTTTGAGCTGCACGCTGGCCGG
TAGCCCGCGTATTGTTGGTAAGAAGATCACTTGGGCTCGTGATACCAACGGTGTTTGGACCTGCTCGACTGATATCGCGA
CTGGTGATGAGGCAAAGTTCGCTCCAGCTACCTGTCAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3T0G1A3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.966

99.315

0.486
  pilA Pseudomonas aeruginosa PAK

43.137

100

0.452
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.781

100

0.418
  pilA Vibrio cholerae strain A1552

41.781

100

0.418
  pilA Vibrio cholerae C6706

41.781

100

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.667

98.63

0.411
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.259

97.945

0.404
  comP Acinetobacter baylyi ADP1

36.076

100

0.39
  pilE Neisseria gonorrhoeae strain FA1090

34.783

100

0.384
  pilA/pilA1 Eikenella corrodens VA1

36.129

100

0.384
  pilA Ralstonia pseudosolanacearum GMI1000

33.133

100

0.377
  pilE Neisseria gonorrhoeae MS11

33.54

100

0.37

Multiple sequence alignment