Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VU14_RS08040 Genome accession   NZ_CP011100
Coordinates   1749322..1750461 (-) Length   379 a.a.
NCBI ID   WP_011706550.1    Uniprot ID   A0AAI9Q2R8
Organism   Aeromonas hydrophila strain AH10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1744322..1755461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VU14_RS08020 (VU14_08020) feoB 1744529..1746802 (+) 2274 WP_045789456.1 Fe(2+) transporter permease subunit FeoB -
  VU14_RS08025 (VU14_08025) - 1746799..1747041 (+) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  VU14_RS08030 (VU14_08030) - 1747153..1747632 (-) 480 WP_045789457.1 WbuC family cupin fold metalloprotein -
  VU14_RS08035 (VU14_08035) glgC 1747768..1749039 (-) 1272 WP_045789458.1 glucose-1-phosphate adenylyltransferase -
  VU14_RS08040 (VU14_08040) pilU 1749322..1750461 (-) 1140 WP_011706550.1 type IVa pilus ATPase TapW Machinery gene
  VU14_RS08045 (VU14_08045) - 1750522..1750878 (-) 357 WP_005302630.1 DMT family protein -
  VU14_RS08050 (VU14_08050) - 1750992..1751723 (+) 732 WP_029300033.1 substrate-binding periplasmic protein -
  VU14_RS08055 (VU14_08055) btuC 1751824..1752831 (+) 1008 WP_045789459.1 vitamin B12 ABC transporter permease BtuC -
  VU14_RS08060 (VU14_08060) - 1752818..1753603 (+) 786 WP_045789460.1 ATP-binding cassette domain-containing protein -
  VU14_RS08065 (VU14_08065) aac(6') 1753625..1754083 (-) 459 WP_045789461.1 aminoglycoside 6'-N-acetyltransferase -
  VU14_RS08070 (VU14_08070) - 1754090..1755154 (-) 1065 WP_045789462.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42264.32 Da        Isoelectric Point: 6.4290

>NTDB_id=142136 VU14_RS08040 WP_011706550.1 1749322..1750461(-) (pilU) [Aeromonas hydrophila strain AH10]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTSDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=142136 VU14_RS08040 WP_011706550.1 1749322..1750461(-) (pilU) [Aeromonas hydrophila strain AH10]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAACGGCGGCCTGCGTCCGCTCAGTGAGACGCCGCTCGAACCCGGCGAGGTGGCCAGAATAGCCGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCCCAGATCGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAAATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTGCTGCTCGATACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAATCCACCTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACTATCGAAGAT
CCGGTGGAGTTCGTGCACCGTCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
TGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTCATCGGGGAAATTCGCGACCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAGACCGGCCACCTGGCCATCTCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTCAAAACCAGCGACGGTGGCCGCCGGGCGGCAGTGGAGATCATGCTGGGCACCCACACCATTCGCGACATGATCA
AACGCGGCGAATTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCACTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTAGTCGAGGGGGTGATCGACGAGGAAGAGGCGGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCGAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAGCCCACCAAGGACG
AAAAAGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.133

98.945

0.536
  pilU Acinetobacter baylyi ADP1

53.521

93.668

0.501
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361

Multiple sequence alignment