Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RY45_RS14410 Genome accession   NZ_CP010947
Coordinates   3192260..3193399 (+) Length   379 a.a.
NCBI ID   WP_044800324.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain AL06-06 isolate Goldfish     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3187260..3198399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RY45_RS14380 (RY45_14370) - 3187567..3188631 (+) 1065 WP_029303894.1 3-deoxy-7-phosphoheptulonate synthase -
  RY45_RS14385 (RY45_14375) aac(6') 3188638..3189096 (+) 459 WP_044800321.1 aminoglycoside 6'-N-acetyltransferase -
  RY45_RS14390 (RY45_14380) - 3189118..3189903 (-) 786 WP_044800322.1 ATP-binding cassette domain-containing protein -
  RY45_RS14395 (RY45_14385) btuC 3189890..3190897 (-) 1008 WP_044800323.1 vitamin B12 ABC transporter permease BtuC -
  RY45_RS14400 (RY45_14390) - 3190998..3191729 (-) 732 WP_029300033.1 ABC transporter substrate-binding protein -
  RY45_RS14405 (RY45_14395) - 3191843..3192199 (+) 357 WP_005302630.1 DMT family protein -
  RY45_RS14410 (RY45_14400) pilU 3192260..3193399 (+) 1140 WP_044800324.1 type IVa pilus ATPase TapW Machinery gene
  RY45_RS14415 (RY45_14405) glgC 3193682..3194953 (+) 1272 WP_011706551.1 glucose-1-phosphate adenylyltransferase -
  RY45_RS14420 (RY45_14410) - 3195089..3195568 (+) 480 WP_044800325.1 WbuC family cupin fold metalloprotein -
  RY45_RS14425 (RY45_14415) - 3195660..3195902 (-) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  RY45_RS14430 (RY45_14420) feoB 3195899..3198172 (-) 2274 WP_044800326.1 Fe(2+) transporter permease subunit FeoB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42308.37 Da        Isoelectric Point: 6.4290

>NTDB_id=140826 RY45_RS14410 WP_044800324.1 3192260..3193399(+) (pilU) [Aeromonas hydrophila strain AL06-06 isolate Goldfish]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSTNNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=140826 RY45_RS14410 WP_044800324.1 3192260..3193399(+) (pilU) [Aeromonas hydrophila strain AL06-06 isolate Goldfish]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAACGGCGGCCTGCGTCCGCTCAGTGAGACGCCGCTCGAGCCCGGCGAGGTGGCCAGAATAGCCGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCTCAGATCGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAAATCCCCCGCTTCGA
GGATCTGAAACTCCCCCCCGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAATCCACCTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGTCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
TGCCCTGAAGAACACCCTGCGCCAGGCACCGGACGTGATCCTCATCGGTGAAATTCGCGATCGCGAGACCATGGAGCACG
CGCTGGCCTTCTCCGAGACCGGCCACCTGGCAATTTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAGACTACCGATGGTGGACGCCGGGCGGCAGTGGAGATCATGCTGGGTACCCACACCATTCGCGACATGATCA
AACGCGGCGAATTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTAGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCCGACTCGACCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCGAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAGCCCACCAAGGATG
AAAAAGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.133

98.945

0.536
  pilU Acinetobacter baylyi ADP1

53.521

93.668

0.501
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

89.446

0.372
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361

Multiple sequence alignment