Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ACLV7W_RS09945 Genome accession   NZ_OZ217346
Coordinates   2054570..2055511 (-) Length   313 a.a.
NCBI ID   WP_411865882.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis D22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2049570..2060511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7W_RS09905 (SMID22_20370) comYH 2050170..2051123 (-) 954 WP_411865877.1 class I SAM-dependent methyltransferase Machinery gene
  ACLV7W_RS09910 (SMID22_20380) - 2051184..2051771 (-) 588 WP_411865878.1 class I SAM-dependent methyltransferase -
  ACLV7W_RS09915 (SMID22_20390) comGG/cglG 2051804..2052217 (-) 414 WP_322741434.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV7W_RS09920 (SMID22_20400) comGF/cglF 2052195..2052656 (-) 462 WP_411865879.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV7W_RS09925 (SMID22_20410) comGE/cglE 2052619..2052921 (-) 303 WP_411865880.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV7W_RS09930 (SMID22_20420) comGD/cglD 2052884..2053318 (-) 435 WP_411865881.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV7W_RS09935 (SMID22_20430) comGC/cglC 2053281..2053604 (-) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  ACLV7W_RS09940 (SMID22_20440) comGB/cglB 2053606..2054622 (-) 1017 WP_411865944.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV7W_RS09945 (SMID22_20450) comGA/cglA/cilD 2054570..2055511 (-) 942 WP_411865882.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV7W_RS09950 (SMID22_20460) - 2055590..2055955 (-) 366 WP_000286409.1 DUF1033 family protein -
  ACLV7W_RS09955 - 2055982..2056206 (-) 225 WP_411865883.1 hypothetical protein -
  ACLV7W_RS09960 (SMID22_20480) - 2056167..2057693 (+) 1527 WP_411865884.1 IS1182 family transposase -
  ACLV7W_RS09970 (SMID22_20520) rplQ 2059423..2059809 (-) 387 WP_000331493.1 50S ribosomal protein L17 -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35632.66 Da        Isoelectric Point: 5.8521

>NTDB_id=1170473 ACLV7W_RS09945 WP_411865882.1 2054570..2055511(-) (comGA/cglA/cilD) [Streptococcus mitis isolate S. mitis D22]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGCYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYE
YDQKMVSLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQEDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSDTARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSKEELAVVLQGVCYQRLIGGGGIVDFASKDYQEHQPTSWNEQIDQLLKDGYITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1170473 ACLV7W_RS09945 WP_411865882.1 2054570..2055511(-) (comGA/cglA/cilD) [Streptococcus mitis isolate S. mitis D22]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCCGCTCGTAAAAAAGGAGCGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTGGGAGACGAGCGCTGTAAAATTGGTTGCTATGATTTCGAAAAGTTTGCGGCCG
TCATCAGTCATTTCAAGTTTGTGGCAGGTATGAATGTTGGAGAAAAGCGACGTAGTCAGCTAGGTTCCTGTGATTATGAA
TATGATCAGAAGATGGTATCCCTACGTTTATCTACTGTAGGCGATTATCGAGGTCATGAGAGTTTAGTTATTCGCTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAACTGGGCAAGCAGTACAGGCAACGGGGACTAT
ATCTTTTTGCTGGTCCAGTCGGCAGTGGTAAGACGACCTTGATGCACGAATTAGCCAAGTCTCTTTTTAAGGGACAACAA
GTCATGTCCATTGAAGATCCGGTAGAAATCAAACAAGAGGATATGCTCCAGTTGCAGTTGAATGAAGCAATCGGACTAAC
CTATGAAAATCTCATCAAACTGTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGGGACAGTGATACGG
CGCGTGCAGTGGTTAGAGCTAGTTTGACAGGGGCAACAGTCTTTTCAACCATTCATGCCAAGAGTATTCGAGGTGTTTAT
GAACGCCTGCTGGAGTTGGGTGTGAGTAAGGAGGAATTAGCAGTCGTTCTACAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAACAGATTGATCAGC
TTCTTAAAGATGGATATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.125

100

0.971
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

95.208

100

0.952
  comGA/cglA/cilD Streptococcus pneumoniae D39

95.208

100

0.952
  comGA/cglA/cilD Streptococcus pneumoniae R6

95.208

100

0.952
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

95.208

100

0.952
  comYA Streptococcus gordonii str. Challis substr. CH1

78.71

99.042

0.78
  comYA Streptococcus mutans UA159

65.273

99.361

0.649
  comYA Streptococcus mutans UA140

65.273

99.361

0.649
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.968

99.042

0.604
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55

Multiple sequence alignment