Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   ACLV7W_RS09940 Genome accession   NZ_OZ217346
Coordinates   2053606..2054622 (-) Length   338 a.a.
NCBI ID   WP_411865944.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis D22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2048606..2059622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7W_RS09900 (SMID22_20360) - 2048930..2050120 (-) 1191 WP_060627210.1 acetate kinase -
  ACLV7W_RS09905 (SMID22_20370) comYH 2050170..2051123 (-) 954 WP_411865877.1 class I SAM-dependent methyltransferase Machinery gene
  ACLV7W_RS09910 (SMID22_20380) - 2051184..2051771 (-) 588 WP_411865878.1 class I SAM-dependent methyltransferase -
  ACLV7W_RS09915 (SMID22_20390) comGG/cglG 2051804..2052217 (-) 414 WP_322741434.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV7W_RS09920 (SMID22_20400) comGF/cglF 2052195..2052656 (-) 462 WP_411865879.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV7W_RS09925 (SMID22_20410) comGE/cglE 2052619..2052921 (-) 303 WP_411865880.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV7W_RS09930 (SMID22_20420) comGD/cglD 2052884..2053318 (-) 435 WP_411865881.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV7W_RS09935 (SMID22_20430) comGC/cglC 2053281..2053604 (-) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  ACLV7W_RS09940 (SMID22_20440) comGB/cglB 2053606..2054622 (-) 1017 WP_411865944.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV7W_RS09945 (SMID22_20450) comGA/cglA/cilD 2054570..2055511 (-) 942 WP_411865882.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV7W_RS09950 (SMID22_20460) - 2055590..2055955 (-) 366 WP_000286409.1 DUF1033 family protein -
  ACLV7W_RS09955 - 2055982..2056206 (-) 225 WP_411865883.1 hypothetical protein -
  ACLV7W_RS09960 (SMID22_20480) - 2056167..2057693 (+) 1527 WP_411865884.1 IS1182 family transposase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38537.62 Da        Isoelectric Point: 9.5998

>NTDB_id=1170472 ACLV7W_RS09940 WP_411865944.1 2053606..2054622(-) (comGB/cglB) [Streptococcus mitis isolate S. mitis D22]
MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVEIISFLDRSALLDKQCVIQMRTGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSLLALLALTFYKRSSKMRVFSILARLPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQSLQNGREFSQTIATYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1170472 ACLV7W_RS09940 WP_411865944.1 2053606..2054622(-) (comGB/cglB) [Streptococcus mitis isolate S. mitis D22]
ATGGATATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTGTTTAA
CAATCTCTTTTCCAGCGGTTTTCATCTGGTGGAGATCATCTCCTTTTTAGATAGGAGTGCCTTGTTGGATAAGCAGTGTG
TGATCCAGATGCGTACAGGCTTGTCTCAGGGCAAATCATTCTCAGAAATGATGGAAAGTTTGGGATTTTCAAGTGCCATT
GTCACTCAGTTATCCCTAGCGGAAGTCCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
GGCCAAGGTCAAGAAAAAACTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATCATGCTGG
GGCTACGGAACTACCTACTACCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGCAATCTGCCACAAATCTTT
TTAGGAATGGTAGGGTTTGTTTCTCTACTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGCGCGTCTT
TTCTATCTTAGCACGCCTTCCCTTTCTTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCGCGTGAGTGGGGGA
ATATGATTTCGCAGGGGATGGAGCTGACGCAGATTTTTCAGATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAATCCCTACAAAATGGCCGCGAATTTTCTCAAACCATAGCAACCTATCCTTTCTTTAAAAAGGA
GTTGAGTCTCATTATTGAGTATGGTGAAGTCAAGTCCAAGCTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTTAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTGATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

97.633

100

0.976
  comGB/cglB Streptococcus mitis NCTC 12261

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae Rx1

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae D39

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae R6

95.858

100

0.959
  comGB/cglB Streptococcus pneumoniae TIGR4

95.858

100

0.959
  comYB Streptococcus gordonii str. Challis substr. CH1

72.619

99.408

0.722
  comYB Streptococcus mutans UA140

58.544

93.491

0.547
  comYB Streptococcus mutans UA159

58.544

93.491

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

51.198

98.817

0.506

Multiple sequence alignment