Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ACLV7D_RS01080 Genome accession   NZ_OZ217345
Coordinates   208917..209858 (+) Length   313 a.a.
NCBI ID   WP_164226419.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis F22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 203917..214858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7D_RS01060 (SMIF22_02050) - 203983..205800 (+) 1818 WP_173234894.1 acyltransferase family protein -
  ACLV7D_RS01065 (SMIF22_02060) nagA 205952..207103 (+) 1152 WP_173234892.1 N-acetylglucosamine-6-phosphate deacetylase -
  ACLV7D_RS01070 (SMIF22_02070) - 207266..208324 (+) 1059 WP_000649461.1 zinc-dependent alcohol dehydrogenase family protein -
  ACLV7D_RS01075 (SMIF22_02080) - 208475..208840 (+) 366 WP_045606653.1 DUF1033 family protein -
  ACLV7D_RS01080 (SMIF22_02090) comGA/cglA/cilD 208917..209858 (+) 942 WP_164226419.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV7D_RS01085 (SMIF22_02100) comGB/cglB 209806..210822 (+) 1017 WP_112445357.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV7D_RS01090 (SMIF22_02110) comGC/cglC 210824..211147 (+) 324 WP_112445359.1 comG operon protein ComGC Machinery gene
  ACLV7D_RS01095 (SMIF22_02120) comGD/cglD 211110..211544 (+) 435 WP_164226418.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV7D_RS01100 (SMIF22_02130) comGE/cglE 211507..211809 (+) 303 WP_191994109.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV7D_RS01105 (SMIF22_02140) comGF/cglF 211772..212233 (+) 462 WP_112445365.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV7D_RS01110 (SMIF22_02150) comGG/cglG 212211..212624 (+) 414 WP_112445367.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV7D_RS01115 (SMIF22_02160) - 212657..213244 (+) 588 WP_164226417.1 class I SAM-dependent methyltransferase -
  ACLV7D_RS01120 (SMIF22_02170) comYH 213305..214258 (+) 954 WP_218764767.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35588.53 Da        Isoelectric Point: 5.6366

>NTDB_id=1170331 ACLV7D_RS01080 WP_164226419.1 208917..209858(+) (comGA/cglA/cilD) [Streptococcus mitis isolate S. mitis F22]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELYMRVGDERCRIGCYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIDELGKQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1170331 ACLV7D_RS01080 WP_164226419.1 208917..209858(+) (comGA/cglA/cilD) [Streptococcus mitis isolate S. mitis F22]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGAGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTTATATGAGGGTGGGAGACGAGCGCTGTAGAATTGGTTGTTATGATTTTGAAAAGTTTGCGGCCG
TCATCAGCCACTTCAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAGCGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCATCTCTACGTCTATCTACTGTAGGCGATTATCGAGGGCACGAGAGTTTAGTTATTCGCTTGTT
GCATGATGAGGAGCAGGATTTGCATTTTTGGTTTCAGGATATTGATGAACTGGGCAAGCAGTACAGGCAACGTGGTCTCT
ATCTCTTTGCTGGTCCAGTCGGAAGTGGCAAGACGACCTTGATGCATGAATTGGCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATAGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTCCAGTTGCAGTTGAATGAAGCGATTGGGCTTAC
CTATGAAAATCTGATTAAACTTTCCTTACGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGGGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATTCGAGGTGTTTAT
GAGCGCCTGTTGGAGTTGGGTGTGAGTGAGGAGGAATTGGCAGTCGTTCTGCAAGGAGTCTGCTATCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCCAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAGCAGATTGATCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.764

100

0.978
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae D39

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae R6

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

95.847

100

0.958
  comYA Streptococcus gordonii str. Challis substr. CH1

79.355

99.042

0.786
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.769

99.681

0.556
  comGA Latilactobacillus sakei subsp. sakei 23K

42.279

86.901

0.367

Multiple sequence alignment