Detailed information NTG1170336

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: ACLV7D_RS01105
• Genome accession: NZ_OZ217345
• Coordinates: 211772..212233 (+)
• Length: 153 a.a.
• NCBI ID: WP_112445365.1
• Uniprot ID: -
• Organism: Streptococcus mitis isolate S. mitis F22
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 206772..217233

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLV7D_RS01070 (SMIF22_02070)	-	207266..208324 (+)	1059	WP_000649461.1	zinc-dependent alcohol dehydrogenase family protein	-
ACLV7D_RS01075 (SMIF22_02080)	-	208475..208840 (+)	366	WP_045606653.1	DUF1033 family protein	-
ACLV7D_RS01080 (SMIF22_02090)	comGA/cglA/cilD	208917..209858 (+)	942	WP_164226419.1	competence type IV pilus ATPase ComGA	Machinery gene
ACLV7D_RS01085 (SMIF22_02100)	comGB/cglB	209806..210822 (+)	1017	WP_112445357.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACLV7D_RS01090 (SMIF22_02110)	comGC/cglC	210824..211147 (+)	324	WP_112445359.1	comG operon protein ComGC	Machinery gene
ACLV7D_RS01095 (SMIF22_02120)	comGD/cglD	211110..211544 (+)	435	WP_164226418.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACLV7D_RS01100 (SMIF22_02130)	comGE/cglE	211507..211809 (+)	303	WP_191994109.1	competence type IV pilus minor pilin ComGE	Machinery gene
ACLV7D_RS01105 (SMIF22_02140)	comGF/cglF	211772..212233 (+)	462	WP_112445365.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACLV7D_RS01110 (SMIF22_02150)	comGG/cglG	212211..212624 (+)	414	WP_112445367.1	competence type IV pilus minor pilin ComGG	Machinery gene
ACLV7D_RS01115 (SMIF22_02160)	-	212657..213244 (+)	588	WP_164226417.1	class I SAM-dependent methyltransferase	-
ACLV7D_RS01120 (SMIF22_02170)	comYH	213305..214258 (+)	954	WP_218764767.1	class I SAM-dependent methyltransferase	Machinery gene
ACLV7D_RS01125 (SMIF22_02180)	-	214308..215498 (+)	1191	WP_218764766.1	acetate kinase	-
ACLV7D_RS01130 (SMIF22_02190)	rnpA	215646..216017 (+)	372	WP_112445373.1	ribonuclease P protein component	-
ACLV7D_RS01135 (SMIF22_02200)	-	215992..216816 (+)	825	WP_218764765.1	membrane protein insertase YidC	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17941.51 Da
• Isoelectric Point: 6.9881

>NTDB_id=1170336 ACLV7D_RS01105 WP_112445365.1 211772..212233(+) (comGF/cglF) [Streptococcus mitis isolate S. mitis F22]
MVQNSCWLSESHKVKAFTLLESLIALIVISGSLLLFRAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEDN
RIYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVRFRFQFQKGLEREFIYRVEKEKS

Nucleotide

• Length: 462 bp

>NTDB_id=1170336 ACLV7D_RS01105 WP_112445365.1 211772..212233(+) (comGF/cglF) [Streptococcus mitis isolate S. mitis F22]
ATGGTTCAGAACAGTTGTTGGCTATCAGAGAGCCATAAGGTTAAGGCTTTTACCTTATTAGAATCCCTGATTGCCCTCAT
TGTCATCAGTGGAAGCTTACTCCTCTTTCGAGCCATGAGTCAGCTCCTCATTTCAGAAGTCCGCTACCAGCAACAAAGCG
AACAAAAAGAGTGGCTCTTGTTTGTGGACCAGCTGGAGGCAGAATTAGACCGTTCGCAGTTTGAAAAAGTGGAAGACAAT
CGCATTTATATGAAGCAGGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGGAAAACCGATGCCAG
TGGACGGGGTTATCAGCCTATGGTTTATGGTCTCAAATCTGCGCAGATTACAGAGGACAATCAACTGGTGCGCTTTCGTT
TTCAATTCCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis SK321	95.425	100	0.954
comGF/cglF	Streptococcus mitis NCTC 12261	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae Rx1	91.503	100	0.915
comGF/cglF	Streptococcus pneumoniae D39	91.503	100	0.915
comGF/cglF	Streptococcus pneumoniae R6	91.503	100	0.915
comGF/cglF	Streptococcus pneumoniae TIGR4	91.503	100	0.915