Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   ACLV7D_RS01085 Genome accession   NZ_OZ217345
Coordinates   209806..210822 (+) Length   338 a.a.
NCBI ID   WP_112445357.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis F22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 204806..215822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7D_RS01065 (SMIF22_02060) nagA 205952..207103 (+) 1152 WP_173234892.1 N-acetylglucosamine-6-phosphate deacetylase -
  ACLV7D_RS01070 (SMIF22_02070) - 207266..208324 (+) 1059 WP_000649461.1 zinc-dependent alcohol dehydrogenase family protein -
  ACLV7D_RS01075 (SMIF22_02080) - 208475..208840 (+) 366 WP_045606653.1 DUF1033 family protein -
  ACLV7D_RS01080 (SMIF22_02090) comGA/cglA/cilD 208917..209858 (+) 942 WP_164226419.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV7D_RS01085 (SMIF22_02100) comGB/cglB 209806..210822 (+) 1017 WP_112445357.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV7D_RS01090 (SMIF22_02110) comGC/cglC 210824..211147 (+) 324 WP_112445359.1 comG operon protein ComGC Machinery gene
  ACLV7D_RS01095 (SMIF22_02120) comGD/cglD 211110..211544 (+) 435 WP_164226418.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV7D_RS01100 (SMIF22_02130) comGE/cglE 211507..211809 (+) 303 WP_191994109.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV7D_RS01105 (SMIF22_02140) comGF/cglF 211772..212233 (+) 462 WP_112445365.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV7D_RS01110 (SMIF22_02150) comGG/cglG 212211..212624 (+) 414 WP_112445367.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV7D_RS01115 (SMIF22_02160) - 212657..213244 (+) 588 WP_164226417.1 class I SAM-dependent methyltransferase -
  ACLV7D_RS01120 (SMIF22_02170) comYH 213305..214258 (+) 954 WP_218764767.1 class I SAM-dependent methyltransferase Machinery gene
  ACLV7D_RS01125 (SMIF22_02180) - 214308..215498 (+) 1191 WP_218764766.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38521.46 Da        Isoelectric Point: 9.6075

>NTDB_id=1170332 ACLV7D_RS01085 WP_112445357.1 209806..210822(+) (comGB/cglB) [Streptococcus mitis isolate S. mitis F22]
MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRTGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSVGVLLALTFYKRSSKMRVFSILARLPFFGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAHALQNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYHNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1170332 ACLV7D_RS01085 WP_112445357.1 209806..210822(+) (comGB/cglB) [Streptococcus mitis isolate S. mitis F22]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCTTGTTTAA
CAATCTCTTTTCCAGTGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCCTTATTGGACAAGCAGTGTG
TGACCCAGATGCGCACGGGCTTGTCTCAAGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGCTTTTCAAGTGCCATT
GTCACTCAGTTATCCCTAGCGGAAGTCCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTAAAGAAAAAATTAATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GACTACGGAACTATCTACTACCACAACTGGATAGCAGCAATATTGCCACCCAAATTATCGGAAATCTGCCACAAATCTTT
CTAGGAATGGTAGGGTTTGTTTCAGTAGGTGTCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGCGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTTTTGGAATCTTTGTGCAGACTTATTTGACAGCCTATTATGCGCGTGAGTGGGGGA
ATATGATTTCGCAGGGAATGGAGTTGACGCAGATTTTTCAGATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATTTGGCTCATGCTCTCCAAAATGGTCGAGAATTTTCTCAAACTATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATCGAGTATGGGGAAGTCAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTCTTTATCTTTGTGGCTCTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCACAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis NCTC 12261

97.337

100

0.973
  comGB/cglB Streptococcus pneumoniae Rx1

96.154

100

0.962
  comGB/cglB Streptococcus pneumoniae D39

96.154

100

0.962
  comGB/cglB Streptococcus pneumoniae R6

96.154

100

0.962
  comGB/cglB Streptococcus pneumoniae TIGR4

96.154

100

0.962
  comGB/cglB Streptococcus mitis SK321

95.858

100

0.959
  comYB Streptococcus gordonii str. Challis substr. CH1

71.726

99.408

0.713
  comYB Streptococcus mutans UA140

58.621

94.379

0.553
  comYB Streptococcus mutans UA159

58.621

94.379

0.553
  comGB Lactococcus lactis subsp. cremoris KW2

50.599

98.817

0.5

Multiple sequence alignment