Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ACLV7H_RS01055 Genome accession   NZ_OZ217340
Coordinates   184495..185436 (+) Length   313 a.a.
NCBI ID   WP_042768810.1    Uniprot ID   A0A4Q2FH30
Organism   Streptococcus oralis isolate S. oralis A22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 179495..190436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7H_RS01035 (SORA22_02030) tgt 179530..180672 (+) 1143 WP_001285237.1 tRNA guanosine(34) transglycosylase Tgt -
  ACLV7H_RS01040 (SORA22_02040) - 180806..182623 (+) 1818 WP_411866132.1 acyltransferase family protein -
  ACLV7H_RS01045 (SORA22_02050) nagA 182764..183915 (+) 1152 WP_411866133.1 N-acetylglucosamine-6-phosphate deacetylase -
  ACLV7H_RS01050 (SORA22_02060) - 184067..184432 (+) 366 WP_000286396.1 DUF1033 family protein -
  ACLV7H_RS01055 (SORA22_02070) comGA/cglA/cilD 184495..185436 (+) 942 WP_042768810.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV7H_RS01060 (SORA22_02080) comGB/cglB 185384..186400 (+) 1017 WP_411867062.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV7H_RS01065 (SORA22_02090) comGC/cglC 186402..186725 (+) 324 WP_220107351.1 competence type IV pilus major pilin ComGC Machinery gene
  ACLV7H_RS01070 (SORA22_02100) comGD/cglD 186688..187122 (+) 435 WP_411866134.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV7H_RS01075 (SORA22_02110) comGE/cglE 187085..187387 (+) 303 WP_045591925.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV7H_RS01080 (SORA22_02120) comGF/cglF 187350..187811 (+) 462 WP_045591923.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV7H_RS01085 (SORA22_02130) comGG/cglG 187789..188202 (+) 414 WP_411866135.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV7H_RS01090 (SORA22_02140) - 188235..188825 (+) 591 WP_411866136.1 class I SAM-dependent methyltransferase -
  ACLV7H_RS01095 (SORA22_02150) comYH 188884..189837 (+) 954 WP_411866137.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35559.46 Da        Isoelectric Point: 5.2808

>NTDB_id=1169926 ACLV7H_RS01055 WP_042768810.1 184495..185436(+) (comGA/cglA/cilD) [Streptococcus oralis isolate S. oralis A22]
MVQEIAQKIIATAKEKKAQDIYFIPKEKSYELHMRVGDERCLVDSYEFDVLAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
HGEKVSSLRLSTVGDYRGYESLVIRLLHDEEQELHFWFQDMNELGEQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQEDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1169926 ACLV7H_RS01055 WP_042768810.1 184495..185436(+) (comGA/cglA/cilD) [Streptococcus oralis isolate S. oralis A22]
ATGGTACAAGAAATTGCACAGAAAATTATTGCGACTGCGAAAGAAAAGAAGGCTCAGGATATCTATTTTATCCCAAAGGA
AAAGTCCTACGAGCTTCACATGCGGGTTGGAGACGAACGGTGTCTAGTTGACTCCTATGAGTTTGATGTTTTAGCTGCTG
TGATTAGTCATTTTAAGTTTGTAGCGGGTATGAACGTAGGAGAGAAGAGGCGTAGTCAGCTGGGGTCTTGTGACTATCAG
CATGGGGAGAAGGTGTCTTCTCTGCGTTTGTCTACCGTAGGAGATTATCGGGGATATGAGAGTTTGGTCATTCGTTTGTT
GCACGATGAGGAGCAGGAACTGCATTTTTGGTTTCAGGATATGAACGAACTGGGCGAGCAGTACAGGCAACGGGGCCTCT
ATCTCTTTGCAGGTCCAGTCGGCAGTGGCAAGACGACCTTGATGCACGAATTAGCCAAGTCCCTCTTTAAGGGACAACAA
GTTATGTCTATTGAAGATCCTGTCGAGATCAAGCAGGAAGACATGCTCCAATTGCAGTTAAATGAGGCGATTGGCTTGAC
CTATGAAAATCTGATCAAACTGTCTCTTCGGCATCGTCCTGACCTCTTGATTATCGGTGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTAGTTAGAGCCAGTTTGACAGGGGCGACAGTCTTTTCAACCATTCATGCTAAAAGTATCCGAGGTGTTTAT
GAGCGCCTTCTGGAGTTGGGTGTGACGGAGGAGGAACTAGCAGTTGTCCTACAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAGCAGATTGATCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Q2FH30

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

89.137

100

0.891
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae D39

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae R6

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

88.179

100

0.882
  comYA Streptococcus gordonii str. Challis substr. CH1

79.032

99.042

0.783
  comYA Streptococcus mutans UA159

66.559

99.361

0.661
  comYA Streptococcus mutans UA140

66.559

99.361

0.661
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.968

99.042

0.604
  comGA Lactococcus lactis subsp. cremoris KW2

55.449

99.681

0.553
  comGA Latilactobacillus sakei subsp. sakei 23K

43.726

84.026

0.367

Multiple sequence alignment