Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACH8EK_RS03255 Genome accession   NZ_OZ197910
Coordinates   610237..611367 (+) Length   376 a.a.
NCBI ID   WP_003704392.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae isolate SGC-24-001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 605237..616367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH8EK_RS03230 - 605683..605832 (+) 150 WP_003687747.1 hypothetical protein -
  ACH8EK_RS03235 - 606056..606163 (+) 108 Protein_631 IS5/IS1182 family transposase -
  ACH8EK_RS03240 lepB 606268..607287 (-) 1020 WP_047922716.1 signal peptidase I -
  ACH8EK_RS03245 lepA 607433..609226 (-) 1794 WP_003687751.1 translation elongation factor 4 -
  ACH8EK_RS03250 - 609368..610069 (-) 702 WP_003687752.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  ACH8EK_RS03255 pilU 610237..611367 (+) 1131 WP_003704392.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACH8EK_RS03260 - 611401..612378 (+) 978 WP_003687755.1 DNA polymerase III subunit delta' -
  ACH8EK_RS03265 - 612382..612732 (+) 351 WP_003687757.1 PilZ domain-containing protein -
  ACH8EK_RS03270 - 612737..613516 (+) 780 WP_047922717.1 TatD family hydrolase -
  ACH8EK_RS03275 - 613542..613756 (+) 215 Protein_639 DUF333 domain-containing protein -
  ACH8EK_RS03280 grxD 613857..614168 (+) 312 WP_003687762.1 Grx4 family monothiol glutaredoxin -
  ACH8EK_RS03285 upp 614274..614900 (+) 627 WP_003687763.1 uracil phosphoribosyltransferase -
  ACH8EK_RS03290 - 614923..615243 (+) 321 WP_003687765.1 DUF2322 family protein -
  ACH8EK_RS03295 - 615412..616053 (+) 642 Protein_643 IS1595 family transposase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42215.32 Da        Isoelectric Point: 6.2891

>NTDB_id=1169709 ACH8EK_RS03255 WP_003704392.1 610237..611367(+) (pilU) [Neisseria gonorrhoeae isolate SGC-24-001]
MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1169709 ACH8EK_RS03255 WP_003704392.1 610237..611367(+) (pilU) [Neisseria gonorrhoeae isolate SGC-24-001]
ATGTTTACCGATGAAAATATGACTGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTTGTGACGACCCATTTCCCGCCCGCTATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGTGCGACGGCGTTGGTATTCCG
CGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGCCTTGAAGGATGTTGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTCGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGATCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATTACCCAGCGCGA
GGTCGGCGTGGACACGGAAAACTGGATGGCGGCGTTGAAAAATACGCTGCGTCAGGCGCCGGATGTGATCCTTATCGGCG
AAATCCGCGACCGTGAAACAATGGACTACGCCATCGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTAC
TACTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGACCAACACCTTTACCAATTGTATGAAAAAGGCGAGATTTCCTTGCAGGATGCCTTGAA
AAATGCCGATTCCGCACATGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCAGGGCACAAAGTTCCGACCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.659

93.617

0.559
  pilU Acinetobacter baylyi ADP1

57.726

91.223

0.527
  pilU Vibrio cholerae strain A1552

54.366

94.415

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Acinetobacter baylyi ADP1

42.727

87.766

0.375
  pilT Pseudomonas aeruginosa PAK

42.378

87.234

0.37
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.463

87.234

0.362

Multiple sequence alignment