Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   AD178_RS09960 Genome accession   NZ_OX244288
Coordinates   1925453..1926394 (-) Length   313 a.a.
NCBI ID   WP_000249549.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PT8105 isolate 2RLC4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1920453..1931394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AD178_RS09920 (SAMEA1463109_02001) comYH 1920939..1921892 (-) 954 WP_000345136.1 class I SAM-dependent methyltransferase Machinery gene
  AD178_RS09925 - 1921953..1922547 (-) 595 Protein_1936 methyltransferase domain-containing protein -
  AD178_RS09930 (SAMEA1463109_02004) comGG/cglG 1922684..1923097 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  AD178_RS09935 (SAMEA1463109_02005) comGF/cglF 1923075..1923536 (-) 462 WP_000250525.1 competence type IV pilus minor pilin ComGF Machinery gene
  AD178_RS09940 (SAMEA1463109_02006) comGE/cglE 1923499..1923801 (-) 303 WP_188109891.1 competence type IV pilus minor pilin ComGE Machinery gene
  AD178_RS09945 (SAMEA1463109_02007) comGD/cglD 1923764..1924168 (-) 405 WP_000588022.1 competence type IV pilus minor pilin ComGD Machinery gene
  AD178_RS09950 (SAMEA1463109_02008) comGC/cglC 1924161..1924487 (-) 327 WP_000738634.1 comG operon protein ComGC Machinery gene
  AD178_RS09955 (SAMEA1463109_02009) comGB/cglB 1924489..1925505 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  AD178_RS09960 (SAMEA1463109_02010) comGA/cglA/cilD 1925453..1926394 (-) 942 WP_000249549.1 competence type IV pilus ATPase ComGA Machinery gene
  AD178_RS09965 (SAMEA1463109_02011) - 1926470..1926835 (-) 366 WP_000286415.1 DUF1033 family protein -
  AD178_RS09970 (SAMEA1463109_02012) - 1926986..1928044 (-) 1059 WP_000649466.1 zinc-dependent alcohol dehydrogenase family protein -
  AD178_RS09975 (SAMEA1463109_02013) nagA 1928207..1929358 (-) 1152 WP_001134461.1 N-acetylglucosamine-6-phosphate deacetylase -
  AD178_RS09980 (SAMEA1463109_02014) - 1929511..1931328 (-) 1818 WP_001220847.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35543.45 Da        Isoelectric Point: 6.0083

>NTDB_id=1154361 AD178_RS09960 WP_000249549.1 1925453..1926394(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PT8105 isolate 2RLC4]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1154361 AD178_RS09960 WP_000249549.1 1925453..1926394(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PT8105 isolate 2RLC4]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment