Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   AD178_RS09955 Genome accession   NZ_OX244288
Coordinates   1924489..1925505 (-) Length   338 a.a.
NCBI ID   WP_074017570.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PT8105 isolate 2RLC4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1919489..1930505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AD178_RS09910 - 1919566..1919697 (-) 132 WP_000768904.1 hypothetical protein -
  AD178_RS09915 (SAMEA1463109_02000) - 1919698..1920888 (-) 1191 WP_000167757.1 acetate kinase -
  AD178_RS09920 (SAMEA1463109_02001) comYH 1920939..1921892 (-) 954 WP_000345136.1 class I SAM-dependent methyltransferase Machinery gene
  AD178_RS09925 - 1921953..1922547 (-) 595 Protein_1936 methyltransferase domain-containing protein -
  AD178_RS09930 (SAMEA1463109_02004) comGG/cglG 1922684..1923097 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  AD178_RS09935 (SAMEA1463109_02005) comGF/cglF 1923075..1923536 (-) 462 WP_000250525.1 competence type IV pilus minor pilin ComGF Machinery gene
  AD178_RS09940 (SAMEA1463109_02006) comGE/cglE 1923499..1923801 (-) 303 WP_188109891.1 competence type IV pilus minor pilin ComGE Machinery gene
  AD178_RS09945 (SAMEA1463109_02007) comGD/cglD 1923764..1924168 (-) 405 WP_000588022.1 competence type IV pilus minor pilin ComGD Machinery gene
  AD178_RS09950 (SAMEA1463109_02008) comGC/cglC 1924161..1924487 (-) 327 WP_000738634.1 comG operon protein ComGC Machinery gene
  AD178_RS09955 (SAMEA1463109_02009) comGB/cglB 1924489..1925505 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  AD178_RS09960 (SAMEA1463109_02010) comGA/cglA/cilD 1925453..1926394 (-) 942 WP_000249549.1 competence type IV pilus ATPase ComGA Machinery gene
  AD178_RS09965 (SAMEA1463109_02011) - 1926470..1926835 (-) 366 WP_000286415.1 DUF1033 family protein -
  AD178_RS09970 (SAMEA1463109_02012) - 1926986..1928044 (-) 1059 WP_000649466.1 zinc-dependent alcohol dehydrogenase family protein -
  AD178_RS09975 (SAMEA1463109_02013) nagA 1928207..1929358 (-) 1152 WP_001134461.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38482.48 Da        Isoelectric Point: 9.6162

>NTDB_id=1154360 AD178_RS09955 WP_074017570.1 1924489..1925505(-) (comGB/cglB) [Streptococcus pneumoniae strain PT8105 isolate 2RLC4]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1154360 AD178_RS09955 WP_074017570.1 1924489..1925505(-) (comGB/cglB) [Streptococcus pneumoniae strain PT8105 isolate 2RLC4]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACTCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae D39

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae R6

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae TIGR4

98.521

100

0.985
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment