Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M7777_RS00450 Genome accession   NZ_OV024757
Coordinates   87240..88496 (-) Length   418 a.a.
NCBI ID   WP_034350111.1    Uniprot ID   -
Organism   Deinococcus radiodurans isolate DRR11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 82240..93496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M7777_RS00420 - 82444..82890 (+) 447 WP_051618937.1 hypothetical protein -
  M7777_RS16265 - 82887..83180 (+) 294 WP_346732442.1 ADP-ribosylglycohydrolase family protein -
  M7777_RS00425 - 83219..83887 (+) 669 WP_346732443.1 ADP-ribosylglycohydrolase family protein -
  M7777_RS00430 - 83884..84441 (+) 558 WP_010888592.1 nucleotidyltransferase family protein -
  M7777_RS00435 - 84516..85715 (+) 1200 WP_010888593.1 acetyl-CoA C-acetyltransferase -
  M7777_RS00440 - 85904..86587 (+) 684 WP_028328057.1 ankyrin repeat domain-containing protein -
  M7777_RS00445 - 86665..87177 (+) 513 WP_010888595.1 hypothetical protein -
  M7777_RS00450 pilT 87240..88496 (-) 1257 WP_034350111.1 type IV pilus twitching motility protein PilT Machinery gene
  M7777_RS00455 pilB 88565..91228 (-) 2664 WP_027479822.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  M7777_RS00460 - 91408..91932 (-) 525 WP_010888598.1 YqeG family HAD IIIA-type phosphatase -
  M7777_RS00465 pgeF 91929..92690 (-) 762 WP_010888599.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 44834.13 Da        Isoelectric Point: 6.7744

>NTDB_id=1151694 M7777_RS00450 WP_034350111.1 87240..88496(-) (pilT) [Deinococcus radiodurans isolate DRR11]
MTSGIDITDILRLAADKGASDIILAAGLPPQFKLSGDYDAQGFEPLSASDTRKLMYSMMNERQQRIFEEKRELDFSFALG
DKARFRVNAFMQRGNVGGVMRLIPTTVRTIAEMGLPQTVIDIANAPRGLVLVTGPTGSGKSTTLASMIDHINTTKKLHIL
TIEDPIEFMHPNKASIINQREVGSDTLSFDDALRAALRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPES
IDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPRADGQGRVLAYEILLANPAVRALIREGKTFQITSVMQTGAREGMVT
MDAYLAGLFRRHLITYDKGLERAIDPKEFARLANDASAGLGGASMTPAAPPVPAYNSASSSSTGRGGDFGRGAAAPATPA
APAAGRSEPGRAGGFGRR

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=1151694 M7777_RS00450 WP_034350111.1 87240..88496(-) (pilT) [Deinococcus radiodurans isolate DRR11]
ATGACTTCCGGCATCGACATCACCGATATCCTGCGCCTCGCCGCCGACAAGGGTGCCTCCGACATCATCCTGGCCGCCGG
GCTGCCGCCGCAGTTCAAACTCTCGGGCGACTACGACGCGCAGGGCTTCGAGCCGCTGAGCGCGAGTGACACCCGCAAGC
TGATGTACTCGATGATGAACGAGCGCCAGCAGCGCATCTTCGAGGAAAAGCGCGAACTCGACTTTTCCTTCGCGCTCGGC
GACAAGGCCCGCTTCCGCGTGAACGCCTTCATGCAGCGCGGCAACGTGGGCGGCGTGATGCGCCTCATCCCCACCACCGT
CCGCACGATTGCCGAGATGGGGCTGCCGCAGACGGTGATCGACATCGCCAACGCGCCGCGCGGGCTGGTGCTCGTCACCG
GGCCGACGGGCTCGGGCAAATCGACCACCCTCGCCTCGATGATCGACCACATCAACACGACCAAGAAGCTGCACATCCTG
ACCATCGAAGACCCCATCGAGTTCATGCACCCCAACAAGGCGAGCATCATCAACCAGCGCGAGGTCGGCAGCGACACCCT
GAGCTTCGACGACGCCCTGCGCGCCGCGCTGCGTCAGGCCCCCGACGTGATTCTGGTGGGCGAAATGCGCGACTACGAGA
CCATCAAGGCCGCCGTGACCGCCGCCGAAACCGGGCACCTCGTGATGGGCACGCTGCACACCAACTCCGCGCCCGAATCC
ATCGACCGCATCGTGGACGTGTTTCCCGAGGAGCAGCAGGAACAGATCCGGGTGCAGCTCGCCAACAACCTCGTCGCCGT
GATGACCCAGCAACTGCTGCCGCGCGCCGACGGGCAGGGACGCGTGCTGGCCTACGAAATCCTGCTGGCGAACCCGGCGG
TCCGCGCCCTGATCCGCGAGGGCAAAACCTTCCAGATCACCAGCGTGATGCAGACCGGCGCCCGCGAAGGCATGGTCACG
ATGGACGCCTACCTCGCCGGGCTGTTCCGCCGCCATCTCATCACCTACGACAAGGGGCTGGAACGCGCCATCGACCCCAA
GGAATTCGCCCGTCTCGCCAACGACGCTTCGGCGGGCCTCGGCGGCGCCAGCATGACGCCCGCCGCGCCGCCCGTTCCCG
CCTACAACAGCGCTTCGAGCAGCAGCACTGGGCGTGGTGGTGACTTCGGACGCGGCGCGGCGGCCCCAGCGACGCCCGCT
GCCCCTGCCGCAGGCCGCAGCGAACCGGGACGCGCCGGAGGCTTTGGCCGCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

100

100

1
  pilT Neisseria meningitidis 8013

53.644

82.057

0.44
  pilT Pseudomonas stutzeri DSM 10701

52.464

82.536

0.433
  pilT Pseudomonas aeruginosa PAK

52.464

82.536

0.433
  pilT Neisseria gonorrhoeae MS11

53.392

81.1

0.433
  pilT Acinetobacter baumannii strain A118

52.174

82.536

0.431
  pilT Acinetobacter baumannii D1279779

52.174

82.536

0.431
  pilT Acinetobacter nosocomialis M2

52.174

82.536

0.431
  pilT Acinetobacter baylyi ADP1

51.884

82.536

0.428
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.988

78.23

0.407
  pilT Vibrio cholerae strain A1552

51.988

78.23

0.407
  pilT Legionella pneumophila strain Lp02

49.275

82.536

0.407
  pilT Legionella pneumophila strain ERS1305867

49.275

82.536

0.407

Multiple sequence alignment