Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CKW04_RS12560 Genome accession   NZ_LT906442
Coordinates   2726842..2727963 (-) Length   373 a.a.
NCBI ID   WP_058479914.1    Uniprot ID   A0A0W1AGP6
Organism   Legionella waltersii strain NCTC13017     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2721842..2732963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW04_RS12535 (SAMEA4504053_02556) - 2722035..2722817 (+) 783 WP_058479918.1 3-hydroxybutyrate dehydrogenase -
  CKW04_RS12540 (SAMEA4504053_02557) motA 2722868..2723791 (+) 924 WP_058479917.1 flagellar motor stator protein MotA -
  CKW04_RS12545 (SAMEA4504053_02558) motB 2723804..2724751 (+) 948 WP_065235653.1 flagellar motor protein MotB -
  CKW04_RS12550 (SAMEA4504053_02559) - 2724745..2725182 (-) 438 WP_058479916.1 hypothetical protein -
  CKW04_RS12555 (SAMEA4504053_02560) - 2725540..2726838 (+) 1299 WP_058479915.1 serine/threonine transporter -
  CKW04_RS12560 (SAMEA4504053_02561) pilU 2726842..2727963 (-) 1122 WP_058479914.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CKW04_RS12565 (SAMEA4504053_02562) - 2728365..2730311 (-) 1947 WP_058479913.1 L-gulono-gamma-lactone oxidase -
  CKW04_RS12570 (SAMEA4504053_02563) radA 2730562..2731920 (-) 1359 WP_058479912.1 DNA repair protein RadA -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41841.19 Da        Isoelectric Point: 5.6593

>NTDB_id=1147247 CKW04_RS12560 WP_058479914.1 2726842..2727963(-) (pilU) [Legionella waltersii strain NCTC13017]
MDITPFFKLMVDKGASDLFFSVGAIPHIKIEGIIAPVGQTPLQSQQMAEIVLSIMNDDQQKEFEAKMEINLAFSLVGIGR
FRINVFRQRGEVAMVIRYIKGEIPSIEDLNLPQILKTLVMELRGLILVVGSTGSGKSTTLASMLNYRNENHRGHILTIED
PIEYLHKHKKSIVDQREVGIDTLSYESALKNAMREAPDVILIGEIRDKDTMKHAIAYAETGHLCISTLHSNNANQALDRV
INFFPEDARHQLLLDLSLNLKAIISLRLIPGIANQRVAAVEVLINSPYISDLIEKGKIDDIKEVMARSKEQGMQTFDQAL
LDLYQGGKISKENTIKFADSKNNVGLQIRLNEEAKYDENDGKLQIQSDDLDKY

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1147247 CKW04_RS12560 WP_058479914.1 2726842..2727963(-) (pilU) [Legionella waltersii strain NCTC13017]
ATGGACATAACACCATTTTTTAAGCTCATGGTCGACAAAGGTGCTTCTGACTTGTTTTTTAGTGTTGGAGCGATACCCCA
CATTAAAATCGAAGGCATCATTGCCCCAGTTGGACAGACCCCTCTTCAATCCCAACAAATGGCTGAAATCGTGTTGTCCA
TCATGAATGATGATCAACAAAAAGAATTCGAAGCTAAAATGGAAATTAATTTGGCATTCTCATTGGTGGGGATAGGGCGA
TTCAGGATTAATGTGTTCAGGCAACGTGGCGAAGTGGCAATGGTCATTCGTTACATCAAAGGTGAAATACCATCTATTGA
AGATTTGAATTTGCCTCAAATATTAAAAACCTTGGTGATGGAATTACGTGGTTTAATTTTAGTAGTTGGTTCTACTGGTT
CAGGTAAATCAACGACCTTGGCTTCTATGCTGAATTACAGAAATGAGAATCATAGAGGACATATATTGACGATAGAAGAC
CCTATTGAGTATTTACACAAACATAAAAAATCGATTGTCGACCAACGTGAAGTTGGAATTGACACATTGAGTTATGAAAG
TGCTTTAAAAAATGCAATGCGAGAGGCTCCCGATGTTATTTTAATTGGGGAGATTAGAGATAAAGACACTATGAAGCATG
CTATAGCTTATGCTGAGACAGGGCATTTATGCATATCAACACTTCACTCAAACAATGCCAACCAGGCTCTAGATAGGGTC
ATTAACTTTTTTCCTGAAGATGCAAGACACCAGCTTTTACTTGATTTATCTTTGAATTTAAAGGCAATTATTTCTTTGCG
CTTAATTCCAGGTATTGCTAATCAGCGAGTAGCTGCCGTAGAGGTCTTAATTAACTCACCGTATATTTCCGATTTGATTG
AGAAAGGCAAGATCGATGACATTAAAGAAGTCATGGCAAGAAGCAAAGAACAAGGCATGCAAACTTTTGATCAGGCCTTG
CTTGATTTGTATCAAGGGGGAAAAATCAGCAAAGAAAATACCATTAAATTTGCTGATTCTAAAAATAACGTGGGTTTGCA
AATCAGGCTAAATGAAGAAGCAAAATACGATGAAAATGATGGAAAATTGCAAATTCAGTCTGATGATTTAGATAAATATT
AG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W1AGP6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

54.494

95.442

0.52
  pilU Pseudomonas stutzeri DSM 10701

54.085

95.174

0.515
  pilU Vibrio cholerae strain A1552

47.671

97.855

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.785

87.399

0.391
  pilT Pseudomonas aeruginosa PAK

42.643

89.276

0.381
  pilT Legionella pneumophila strain ERS1305867

41.52

91.689

0.381
  pilT Legionella pneumophila strain Lp02

41.52

91.689

0.381
  pilT Acinetobacter nosocomialis M2

42.042

89.276

0.375
  pilT Acinetobacter baumannii D1279779

42.042

89.276

0.375
  pilT Acinetobacter baumannii strain A118

42.042

89.276

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.525

91.957

0.373
  pilT Neisseria gonorrhoeae MS11

41.441

89.276

0.37
  pilT Neisseria meningitidis 8013

41.441

89.276

0.37

Multiple sequence alignment