Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LLA12_RS11745 Genome accession   NZ_LT599049
Coordinates   2383865..2384803 (-) Length   312 a.a.
NCBI ID   WP_032946550.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain A12     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2378865..2389803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLA12_RS11705 (LLA12_02482) - 2379753..2380595 (-) 843 WP_068872849.1 metal ABC transporter substrate-binding protein -
  LLA12_RS11710 (LLA12_02483) - 2380592..2381029 (-) 438 WP_021721874.1 zinc-dependent MarR family transcriptional regulator -
  LLA12_RS11715 (LLA12_02484) comGG 2381110..2381385 (-) 276 WP_021721873.1 hypothetical protein Machinery gene
  LLA12_RS11720 (LLA12_02485) comGF 2381433..2381879 (-) 447 WP_021721872.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLA12_RS11725 (LLA12_02486) comGE 2381842..2382138 (-) 297 WP_021721871.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLA12_RS11730 (LLA12_02487) comGD 2382110..2382541 (-) 432 WP_080651959.1 competence type IV pilus minor pilin ComGD Machinery gene
  LLA12_RS11735 (LLA12_02488) comGC 2382501..2382884 (-) 384 WP_080651958.1 competence type IV pilus major pilin ComGC Machinery gene
  LLA12_RS11740 (LLA12_02489) comGB 2382898..2383971 (-) 1074 WP_080651957.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLA12_RS11745 (LLA12_02490) comGA 2383865..2384803 (-) 939 WP_032946550.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35593.86 Da        Isoelectric Point: 6.4012

>NTDB_id=1144892 LLA12_RS11745 WP_032946550.1 2383865..2384803(-) (comGA) [Lactococcus lactis subsp. lactis strain A12]
MIPKMAKELIQKAIDRGASDIYLIASKEKYKLYFRQMTARVLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFELARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHAASVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTSTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1144892 LLA12_RS11745 WP_032946550.1 2383865..2384803(-) (comGA) [Lactococcus lactis subsp. lactis strain A12]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAAAGCAATTGATAGAGGTGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCGCGTGTTCTGGTAGAAGAAATTGGATTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTACGTCTTTCAACAGTGGGGGATTTCGAGGGTAATGAATCCTTGGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCTGGTCCAGTAGGATCGGGCAAAACATCTTTAATGTTTGAGTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGACTCTGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTATTTTCAACCGTTCATGCCGCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGATATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACATCTACTGAAGCCAAGAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

77.564

100

0.776
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

55.305

99.679

0.551
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

54.341

99.679

0.542
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.376

99.679

0.532
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.438

100

0.516

Multiple sequence alignment