Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LLA12_RS11720 Genome accession   NZ_LT599049
Coordinates   2381433..2381879 (-) Length   148 a.a.
NCBI ID   WP_021721872.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain A12     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2376564..2381029 2381433..2381879 flank 404


Gene organization within MGE regions


Location: 2376564..2381879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLA12_RS11695 (LLA12_02480) - 2378036..2378845 (-) 810 WP_032946979.1 metal ABC transporter permease -
  LLA12_RS11700 (LLA12_02481) - 2378838..2379575 (-) 738 WP_021723055.1 metal ABC transporter ATP-binding protein -
  LLA12_RS11705 (LLA12_02482) - 2379753..2380595 (-) 843 WP_068872849.1 metal ABC transporter substrate-binding protein -
  LLA12_RS11710 (LLA12_02483) - 2380592..2381029 (-) 438 WP_021721874.1 zinc-dependent MarR family transcriptional regulator -
  LLA12_RS11715 (LLA12_02484) comGG 2381110..2381385 (-) 276 WP_021721873.1 hypothetical protein Machinery gene
  LLA12_RS11720 (LLA12_02485) comGF 2381433..2381879 (-) 447 WP_021721872.1 competence type IV pilus minor pilin ComGF Machinery gene

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 17156.02 Da        Isoelectric Point: 9.0730

>NTDB_id=1144887 LLA12_RS11720 WP_021721872.1 2381433..2381879(-) (comGF) [Lactococcus lactis subsp. lactis strain A12]
MERKFCDLNLKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQMAISQSDSIKDWQIFCQQMRFELSGTKLDKVEQNFL
YVTKDKKLRFGFMNDDFRKTDDKGQGYQPMLYDIKGAKIQATKNLITIRIDFNQGGERTFIYQFPEDK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=1144887 LLA12_RS11720 WP_021721872.1 2381433..2381879(-) (comGF) [Lactococcus lactis subsp. lactis strain A12]
ATGGAAAGGAAATTTTGCGACTTGAACCTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGATCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAATGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAAGGTAGAACAGAATTTTCTA
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAATGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGGGGCTAAGATTCAAGCTACTAAAAATCTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATCTATCAATTTCCAGAAGACAAATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(48-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

78.014

95.27

0.743
  comYF Streptococcus mutans UA140

48.227

95.27

0.459
  comYF Streptococcus mutans UA159

47.518

95.27

0.453
  comGF/cglF Streptococcus mitis SK321

42.143

94.595

0.399
  comGF/cglF Streptococcus pneumoniae Rx1

40.714

94.595

0.385
  comGF/cglF Streptococcus pneumoniae D39

40.714

94.595

0.385
  comGF/cglF Streptococcus pneumoniae R6

40.714

94.595

0.385
  comGF/cglF Streptococcus pneumoniae TIGR4

40.714

94.595

0.385
  comGF/cglF Streptococcus mitis NCTC 12261

40.714

94.595

0.385

Multiple sequence alignment