Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DQN91_RS08105 Genome accession   NZ_LS483441
Coordinates   1730933..1732069 (-) Length   378 a.a.
NCBI ID   WP_029314740.1    Uniprot ID   -
Organism   Aeromonas caviae strain NCTC12244     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1725933..1737069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN91_RS08080 (NCTC12244_01621) - 1726827..1727384 (-) 558 WP_010675206.1 TMEM165/GDT1 family protein -
  DQN91_RS08085 (NCTC12244_01622) - 1727638..1727967 (-) 330 WP_039041208.1 translation initiation factor -
  DQN91_RS08090 (NCTC12244_01623) - 1728087..1728314 (+) 228 WP_010675204.1 FeoA family protein -
  DQN91_RS08095 (NCTC12244_01624) feoB 1728311..1730581 (+) 2271 WP_042014990.1 Fe(2+) transporter permease subunit FeoB -
  DQN91_RS08100 (NCTC12244_01625) - 1730628..1730870 (+) 243 WP_111736042.1 FeoC-like transcriptional regulator -
  DQN91_RS08105 (NCTC12244_01626) pilU 1730933..1732069 (-) 1137 WP_029314740.1 type IVa pilus ATPase TapW Machinery gene
  DQN91_RS08110 (NCTC12244_01627) - 1732126..1732482 (-) 357 WP_010675200.1 DMT family protein -
  DQN91_RS08115 (NCTC12244_01628) - 1732595..1733326 (+) 732 WP_042014691.1 ABC transporter substrate-binding protein -
  DQN91_RS08120 (NCTC12244_01629) btuC 1733402..1734409 (+) 1008 WP_039041213.1 vitamin B12 ABC transporter permease BtuC -
  DQN91_RS08125 (NCTC12244_01630) - 1734396..1735169 (+) 774 WP_042014689.1 ATP-binding cassette domain-containing protein -
  DQN91_RS08130 (NCTC12244_01631) - 1735377..1736441 (-) 1065 WP_042014687.1 3-deoxy-7-phosphoheptulonate synthase -
  DQN91_RS08135 (NCTC12244_01632) - 1736583..1736963 (+) 381 WP_039040385.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42203.27 Da        Isoelectric Point: 6.6000

>NTDB_id=1141607 DQN91_RS08105 WP_029314740.1 1730933..1732069(-) (pilU) [Aeromonas caviae strain NCTC12244]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLTQEPLGPGEVARIAYDIMDLEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHQKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEVMLGTHTVRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDEGDLF

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1141607 DQN91_RS08105 WP_029314740.1 1730933..1732069(-) (pilU) [Aeromonas caviae strain NCTC12244]
ATGGAATTGAGAGACATGCTGCAGATCCTGGCCAAACAGGACGGTTCCGATCTCTATCTCTCCACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGCGCCCTGCGCCCCCTCACCCAGGAGCCCCTGGGGCCGGGCGAGGTGGCCCGCATCGCCTACGACA
TCATGGATCTCGAGCAGAAACAGCAGTTCGAGAAAGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGGTCAACATCTTCAAGCAGCGCAATGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTCAAACTCCCTCCGGTGCTCCTCGACACCATCATGGAGAAGCGGGGTCTGGTGCTCTTCGTCGGCGGCACGGGGT
CCGGCAAGTCCACGTCGCTCGCGGCCCTCATCGATCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCAGAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
GGCCTTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTCATCGGTGAAATCCGCGATCGGGAGACCATGGAGCATG
CCCTCGCCTTCTCGGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGTTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCAGACCCCAACTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAGACCACGGACGGCGGGCGCCGGGCGGCGGTGGAGGTAATGCTCGGGACCCACACGGTGCGCGATCTCATCA
AACGCGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAAAAATCCAGGGCCCTTGGCATGGTGACCTTCGACAGTACTCTG
TTCGATCTGGTGGTCGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCGGACTCCCAGAACAACCTGCGCCTCAA
GATCAAACTCTGGCGGGAGAAGGGCCAGATTGGCAACAGCGCGGCGGCGAGCGGCTGGAGCCTGGAGCCCACCAAGGACG
AGGGCGACCTCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.4

99.206

0.54
  pilU Acinetobacter baylyi ADP1

52.514

94.709

0.497
  pilU Vibrio cholerae strain A1552

49.858

93.386

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

89.683

0.381
  pilT Vibrio cholerae strain A1552

40.456

92.857

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.456

92.857

0.376
  pilT Legionella pneumophila strain ERS1305867

42.216

88.36

0.373
  pilT Legionella pneumophila strain Lp02

42.216

88.36

0.373
  pilT Pseudomonas aeruginosa PAK

40.988

91.005

0.373
  pilT Pseudomonas stutzeri DSM 10701

40.698

91.005

0.37
  pilT Acinetobacter baumannii strain A118

41.317

88.36

0.365
  pilT Acinetobacter nosocomialis M2

41.317

88.36

0.365
  pilT Acinetobacter baumannii D1279779

41.317

88.36

0.365

Multiple sequence alignment