Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DQM57_RS02120 Genome accession   NZ_LS483369
Coordinates   393925..395055 (+) Length   376 a.a.
NCBI ID   WP_107960157.1    Uniprot ID   -
Organism   Neisseria cinerea strain NCTC10294     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 388925..400055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM57_RS02105 (NCTC10294_00405) lepB 389960..390979 (-) 1020 WP_111726562.1 signal peptidase I -
  DQM57_RS02110 (NCTC10294_00406) lepA 391122..392915 (-) 1794 WP_003677561.1 translation elongation factor 4 -
  DQM57_RS02115 (NCTC10294_00407) - 393059..393760 (-) 702 WP_111726564.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  DQM57_RS02120 (NCTC10294_00408) pilU 393925..395055 (+) 1131 WP_107960157.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DQM57_RS02125 (NCTC10294_00409) - 395090..396067 (+) 978 WP_111726566.1 DNA polymerase III subunit delta' -
  DQM57_RS02130 (NCTC10294_00410) - 396071..396421 (+) 351 WP_111726568.1 PilZ domain-containing protein -
  DQM57_RS02135 (NCTC10294_00411) - 396426..397205 (+) 780 WP_111726570.1 TatD family hydrolase -
  DQM57_RS02145 (NCTC10294_00412) - 397614..398504 (-) 891 WP_111726572.1 LysR family transcriptional regulator -
  DQM57_RS02150 (NCTC10294_00413) - 398681..399259 (+) 579 WP_107859216.1 NAD(P)H-dependent oxidoreductase -
  DQM57_RS02155 (NCTC10294_00414) - 399291..399563 (+) 273 WP_231992117.1 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42126.22 Da        Isoelectric Point: 6.1843

>NTDB_id=1138941 DQM57_RS02120 WP_107960157.1 393925..395055(+) (pilU) [Neisseria cinerea strain NCTC10294]
MFTDENMTAKDELFVWLRHMNKYKGSDLFVTTNFPPAMKVDGTITRITDTPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
TFGHIITIEDPIEFVHDHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLIPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEIMKKST
ALGMQTFDQHLYQLYENGDISLQDALKNADSAHDLRLAVQLRSRRAQSSGPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1138941 DQM57_RS02120 WP_107960157.1 393925..395055(+) (pilU) [Neisseria cinerea strain NCTC10294]
ATGTTTACCGATGAAAATATGACTGCAAAAGACGAGCTGTTTGTATGGTTGCGCCATATGAACAAGTATAAGGGTTCGGA
TTTGTTTGTTACAACCAATTTCCCGCCTGCTATGAAGGTGGACGGAACAATCACCCGTATCACGGATACGCCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAGTTTTCATCGACCAACGAGTGCAAC
TTTGCCATCAGCCTGCCGGATACCAGCCGTTTCCGCGTTAATGCGATGATACAGCGCGGCGCGACGGCATTGGTATTCCG
CGCAATTACCAGCAAAATTCCCAAGTTTGAGAGTTTGAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GCCTGGTGATTTTTGTCGGCGGTACCGGCTCGGGTAAATCGACTTCGCTTGCCTCGCTTATCGATTATCGCAACGAAAAC
ACGTTCGGACACATTATTACTATCGAAGACCCGATTGAGTTTGTCCACGACCATAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTAGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGCCAGGCACCCGATGTCATCCTTATCGGTG
AAATCCGTGACCGCGAAACCATGGATTACGCCATCGCCTTTGCCGAAACGGGTCATTTGTGTATGGCGACGTTGCACGCC
AACAGCACCAACCAGGCACTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCGAACAGTTATTGACGGATTTGTC
GCTCAACCTTCAGGCATTTATTTCACAACGCCTGATTCCGCGAGACGGCGGTAAGGGCAGGGTGGCTGCAGTCGAAGTGC
TGCTCAATTCGCCTTTGATTTCCGAGCTGATTCATAACGGCAATATCCATGAAATCAAGGAAATTATGAAGAAGTCCACG
GCTTTGGGTATGCAGACCTTTGACCAACACCTTTACCAATTGTACGAAAACGGCGATATTTCTTTGCAGGATGCGTTGAA
AAATGCCGATTCGGCGCATGATTTGCGCCTGGCGGTACAGTTGAGGAGCCGCCGCGCACAAAGTTCGGGGCCTGATTTGG
AATTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.942

92.287

0.553
  pilU Acinetobacter baylyi ADP1

57.726

91.223

0.527
  pilU Vibrio cholerae strain A1552

54.039

95.479

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.493

93.883

0.399
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Pseudomonas aeruginosa PAK

42.683

87.234

0.372
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Acinetobacter baylyi ADP1

42.073

87.234

0.367
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.463

87.234

0.362

Multiple sequence alignment