Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACLWA7_RS17195 Genome accession   NZ_LR962646
Coordinates   3972692..3973117 (-) Length   141 a.a.
NCBI ID   WP_053051307.1    Uniprot ID   A0A7U7D8E0
Organism   Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1483     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3968831..3988090 3972692..3973117 within 0


Gene organization within MGE regions


Location: 3968831..3988090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLWA7_RS17180 (X12_003413) - 3968831..3970357 (-) 1527 WP_053051304.1 hypothetical protein -
  ACLWA7_RS17185 (X12_003414) - 3970327..3970896 (-) 570 WP_231103946.1 hypothetical protein -
  ACLWA7_RS17190 (X12_003415) - 3970967..3972556 (-) 1590 WP_053051306.1 phosphoethanolamine transferase -
  ACLWA7_RS17195 (X12_003416) pilA 3972692..3973117 (-) 426 WP_053051307.1 pilin Machinery gene
  ACLWA7_RS17200 (X12_003417) pilC 3973471..3974730 (+) 1260 WP_053051318.1 type II secretion system F family protein Machinery gene
  ACLWA7_RS17205 (X12_003418) - 3974737..3975600 (+) 864 WP_016849610.1 prepilin peptidase -
  ACLWA7_RS17210 (X12_003419) coaE 3975614..3976237 (+) 624 WP_053051308.1 dephospho-CoA kinase -
  ACLWA7_RS17215 (X12_003420) - 3976247..3976485 (+) 239 Protein_3363 SymE family type I addiction module toxin -
  ACLWA7_RS17220 (X12_003421) - 3976589..3977923 (-) 1335 WP_016904089.1 sensor histidine kinase -
  ACLWA7_RS17225 (X12_003422) - 3977916..3978593 (-) 678 WP_006448355.1 response regulator transcription factor -
  ACLWA7_RS17230 (X12_003423) - 3978621..3979098 (-) 478 Protein_3366 hypothetical protein -
  ACLWA7_RS17235 (X12_003424) rimK 3979289..3980194 (-) 906 WP_166767257.1 30S ribosomal protein S6--L-glutamate ligase -
  ACLWA7_RS17240 (X12_003425) glgX 3980685..3982817 (+) 2133 WP_053051310.1 glycogen debranching protein GlgX -
  ACLWA7_RS17245 (X12_003426) - 3983489..3983881 (-) 393 WP_023904915.1 H-NS family nucleoid-associated regulatory protein -
  ACLWA7_RS17250 (X12_003427) - 3983970..3984356 (-) 387 WP_026064907.1 hypothetical protein -
  ACLWA7_RS17255 (X12_003428) - 3984931..3985443 (-) 513 WP_049805061.1 ImmA/IrrE family metallo-endopeptidase -
  ACLWA7_RS17260 (X12_003429) - 3985428..3985634 (-) 207 WP_374057769.1 ImmA/IrrE family metallo-endopeptidase -
  ACLWA7_RS17265 (X12_003430) - 3986178..3986738 (+) 561 WP_102596783.1 hypothetical protein -
  ACLWA7_RS17270 (X12_003431) - 3986810..3988090 (+) 1281 WP_080591431.1 retropepsin-like aspartic protease -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14357.57 Da        Isoelectric Point: 9.0171

>NTDB_id=1134889 ACLWA7_RS17195 WP_053051307.1 3972692..3973117(-) (pilA) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1483]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTTRAKLSEALTMSAPAKLAVTETSSSLGGLAQVTAANSGYAFPGATK
YVSGVTIASGTGQVAVTSTVPNATGTLLLTPTDVGGGQLKWTCSSAINVKYLPAECRNSGT

Nucleotide


Download         Length: 426 bp        

>NTDB_id=1134889 ACLWA7_RS17195 WP_053051307.1 3972692..3973117(-) (pilA) [Xanthomonas arboricola isolate Xanthomonas sp. CPBF 1483]
ATGAAAAAGCAGCAAGGCTTTACACTTATCGAATTGATGATCGTGGTCGCGATCATCGCCATCCTGGCCGCCATCGCACT
GCCGGCTTATCAGGACTACACCACTCGCGCCAAGCTCAGTGAAGCGCTGACGATGTCGGCGCCTGCCAAGCTTGCGGTAA
CGGAGACTTCATCGTCGCTCGGCGGCCTCGCTCAGGTTACTGCGGCAAATTCGGGCTACGCATTCCCGGGTGCCACGAAG
TATGTCAGCGGCGTCACTATTGCCAGTGGCACGGGCCAAGTCGCTGTAACCTCCACCGTGCCGAACGCAACCGGTACCCT
CCTGCTGACCCCGACTGATGTCGGTGGTGGCCAGCTGAAGTGGACCTGCTCTTCCGCCATCAACGTCAAGTACCTGCCGG
CGGAGTGCCGTAACTCGGGCACCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-137)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

42.073

100

0.489
  pilA2 Legionella pneumophila str. Paris

50

96.454

0.482
  pilA2 Legionella pneumophila strain ERS1305867

50

96.454

0.482
  comP Acinetobacter baylyi ADP1

44.898

100

0.468
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.054

100

0.447
  pilE Neisseria gonorrhoeae strain FA1090

39.241

100

0.44
  pilA/pilA1 Eikenella corrodens VA1

40

100

0.426
  pilE Neisseria gonorrhoeae MS11

36.875

100

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.58

97.872

0.397
  pilA Acinetobacter baumannii strain A118

38.095

100

0.397
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.741

95.745

0.39
  pilA Pseudomonas aeruginosa PAK

35.714

100

0.39

Multiple sequence alignment