Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   PSYRMG_RS26570 Genome accession   NZ_CP005970
Coordinates   4582734..4583159 (+) Length   141 a.a.
NCBI ID   WP_044323946.1    Uniprot ID   -
Organism   Pseudomonas syringae UMAF0158     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4577734..4588159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYRMG_RS19995 (PSYRMG_20340) yacG 4577894..4578103 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  PSYRMG_RS20000 (PSYRMG_20345) coaE 4578100..4578723 (-) 624 WP_058397527.1 dephospho-CoA kinase -
  PSYRMG_RS20005 (PSYRMG_20350) pilD 4578720..4579592 (-) 873 WP_058397526.1 A24 family peptidase Machinery gene
  PSYRMG_RS20010 (PSYRMG_20355) pilC 4579594..4580811 (-) 1218 WP_058397525.1 type II secretion system F family protein Machinery gene
  PSYRMG_RS20015 (PSYRMG_20360) pilB 4580814..4582508 (-) 1695 WP_058397524.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSYRMG_RS26570 pilA2 4582734..4583159 (+) 426 WP_044323946.1 pilin Machinery gene
  PSYRMG_RS27930 - 4583300..4583471 (+) 172 Protein_3977 DUF433 domain-containing protein -
  PSYRMG_RS20025 (PSYRMG_20365) - 4583519..4584064 (-) 546 WP_003371273.1 DUF1523 family protein -
  PSYRMG_RS20030 (PSYRMG_20370) - 4584184..4585182 (+) 999 WP_060708664.1 ATP-grasp domain-containing protein -
  PSYRMG_RS20035 (PSYRMG_20375) - 4585237..4586376 (+) 1140 WP_060708665.1 trypsin-like peptidase domain-containing protein -
  PSYRMG_RS20040 (PSYRMG_20380) - 4586443..4587576 (+) 1134 WP_060708666.1 phosphoribosyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14450.49 Da        Isoelectric Point: 8.4743

>NTDB_id=112715 PSYRMG_RS26570 WP_044323946.1 4582734..4583159(+) (pilA2) [Pseudomonas syringae UMAF0158]
MRSQKGFTLIELMIVVAIVGILAAVALPAYQDYTVRAQVAELIGLTDGAKVAVTENYQSTGAFPASNQAAGYGGATGRYT
TSVVIGANGLITATADAVNSNPAIRGLTITLTPAVAAAGNNVVTWTCGGTIDQRFRPTSCR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=112715 PSYRMG_RS26570 WP_044323946.1 4582734..4583159(+) (pilA2) [Pseudomonas syringae UMAF0158]
ATGAGATCACAAAAAGGTTTTACCCTTATTGAATTGATGATTGTTGTTGCAATCGTCGGTATCTTGGCGGCGGTCGCACT
GCCTGCATATCAGGATTATACGGTTCGAGCCCAAGTGGCTGAGCTCATCGGTCTGACTGATGGTGCCAAGGTCGCAGTTA
CTGAAAATTATCAATCAACAGGTGCATTTCCTGCTTCTAACCAAGCTGCTGGCTATGGCGGAGCAACTGGTCGCTATACG
ACTTCTGTAGTGATTGGTGCTAATGGATTGATTACCGCTACAGCTGATGCTGTAAACTCGAACCCTGCAATTCGTGGTCT
GACCATTACGCTAACTCCGGCTGTGGCAGCCGCAGGTAATAATGTCGTTACATGGACATGCGGCGGTACTATTGACCAGC
GTTTCCGTCCGACGAGCTGCCGATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

55.245

100

0.56
  pilA2 Legionella pneumophila strain ERS1305867

55.245

100

0.56
  pilE Neisseria gonorrhoeae MS11

44.654

100

0.504
  pilE Neisseria gonorrhoeae strain FA1090

44.304

100

0.496
  pilA/pilA1 Eikenella corrodens VA1

43.59

100

0.482
  comP Acinetobacter baylyi ADP1

41.611

100

0.44
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.559

100

0.411
  pilA Acinetobacter baumannii strain A118

38

100

0.404
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.437

100

0.397
  pilA Pseudomonas aeruginosa PAK

35

100

0.397
  pilA Haemophilus influenzae 86-028NP

36.986

100

0.383

Multiple sequence alignment