Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSYRMG_RS20010 Genome accession   NZ_CP005970
Coordinates   4579594..4580811 (-) Length   405 a.a.
NCBI ID   WP_058397525.1    Uniprot ID   -
Organism   Pseudomonas syringae UMAF0158     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4574594..4585811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYRMG_RS28155 - 4575899..4576135 (-) 237 WP_316933231.1 DUF3094 family protein -
  PSYRMG_RS19980 (PSYRMG_20325) - 4576020..4576553 (-) 534 WP_054082223.1 MOSC domain-containing protein -
  PSYRMG_RS19985 (PSYRMG_20330) - 4576550..4577179 (-) 630 WP_003345130.1 DUF1780 domain-containing protein -
  PSYRMG_RS19990 (PSYRMG_20335) - 4577307..4577768 (+) 462 WP_058397529.1 hypothetical protein -
  PSYRMG_RS19995 (PSYRMG_20340) yacG 4577894..4578103 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  PSYRMG_RS20000 (PSYRMG_20345) coaE 4578100..4578723 (-) 624 WP_058397527.1 dephospho-CoA kinase -
  PSYRMG_RS20005 (PSYRMG_20350) pilD 4578720..4579592 (-) 873 WP_058397526.1 A24 family peptidase Machinery gene
  PSYRMG_RS20010 (PSYRMG_20355) pilC 4579594..4580811 (-) 1218 WP_058397525.1 type II secretion system F family protein Machinery gene
  PSYRMG_RS20015 (PSYRMG_20360) pilB 4580814..4582508 (-) 1695 WP_058397524.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSYRMG_RS26570 pilA2 4582734..4583159 (+) 426 WP_044323946.1 pilin Machinery gene
  PSYRMG_RS27930 - 4583300..4583471 (+) 172 Protein_3977 DUF433 domain-containing protein -
  PSYRMG_RS20025 (PSYRMG_20365) - 4583519..4584064 (-) 546 WP_003371273.1 DUF1523 family protein -
  PSYRMG_RS20030 (PSYRMG_20370) - 4584184..4585182 (+) 999 WP_060708664.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44091.04 Da        Isoelectric Point: 10.1744

>NTDB_id=112704 PSYRMG_RS20010 WP_058397525.1 4579594..4580811(-) (pilC) [Pseudomonas syringae UMAF0158]
MASKVVKVIVYTWEGVDKKGTKTSGELSGHNLALVKAQLRKQGINPTKVRKKSASIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRTLVNSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPAAVLIVAVIVSGILLIKVVPQFQSVFAGFGAELPTFTLMVIGLSVFIQEWWLAIIGIFFASIFMFK
KSYKKSQKFRDSLDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVIFKNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDSMLDKVATYYEDEVDNMVDSLTSLMEPMIMAILGVVVGGLVIAMYLPIFK
LGNAV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=112704 PSYRMG_RS20010 WP_058397525.1 4579594..4580811(-) (pilC) [Pseudomonas syringae UMAF0158]
ATGGCCAGCAAGGTAGTCAAAGTTATCGTTTACACGTGGGAAGGTGTTGACAAGAAAGGCACTAAAACCAGTGGCGAACT
GAGCGGCCATAACCTGGCGCTCGTAAAGGCCCAGTTACGCAAGCAAGGCATCAATCCTACCAAGGTGCGTAAGAAGTCTG
CGTCGATATTCGGTAAGGGCAAAAAAATCAAGCCATTGGACATCGCGTTTTTTTCTCGGCAGATGGCAACGATGATGAAG
GCTGGCGTACCATTGCTGCAGTCGTTCGACATAATCAGCGAGGGTGCAGAGAACCCGAACATGCGAACATTGGTAAACTC
TCTGAAGCAGGAAGTTTCAGCAGGTAACAGCTTTGCGACGGCTTTGCGGCAAAAGCCCGAATATTTCGACGACCTCTTTT
GCAATCTTGTGGATGCTGGTGAGCAAGCCGGCGCACTTGAGAGTTTGCTGGACCGAGTTGCGAGCTACAAAGAGAAGACC
GAAAAGCTCAAGGCAAAAATCAAAAAGGCTATGACCTATCCCGCTGCAGTATTAATAGTAGCAGTTATAGTTTCAGGAAT
CCTGCTTATCAAAGTTGTACCACAATTTCAATCAGTATTCGCTGGATTCGGAGCAGAGCTGCCGACCTTTACTTTGATGG
TTATTGGACTATCCGTTTTCATACAGGAGTGGTGGCTAGCGATCATCGGGATTTTTTTTGCTTCAATATTTATGTTCAAA
AAATCATATAAAAAGTCTCAAAAATTTCGCGACAGTCTGGATCGTTTTTTATTAAAAGTGCCGATTATAGGACCGCTAAT
TTTTAAATCTTCAGTAGCCCGCTACGCACGCACACTAGCGACGACGTTTGCAGCTGGCGTCCCGTTGGTAGAGGCGCTGG
ATTCAGTCGCAGGAGCGACCGGCAATGTCATATTCAAGAACGCAGTCAACAAGGTCAAGCAGGACGTCTCGACCGGTATG
CAGCTTAACTTCTCAATGCGCTCCACCGGGGTTTTTCCTAGCTTAGCAATTCAGATGACTGCTATAGGTGAGGAGTCCGG
CGCACTTGACAGTATGCTCGATAAAGTTGCGACCTATTACGAGGATGAGGTAGATAACATGGTAGATAGCCTGACCAGTC
TCATGGAGCCTATGATAATGGCAATCCTAGGCGTGGTTGTCGGAGGTTTAGTAATTGCTATGTACTTACCGATTTTTAAA
TTAGGAAACGCAGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.086

100

0.731
  pilC Acinetobacter baylyi ADP1

59.559

100

0.6
  pilC Acinetobacter baumannii D1279779

60.957

98.025

0.598
  pilC Legionella pneumophila strain ERS1305867

52.381

98.519

0.516
  pilG Neisseria gonorrhoeae MS11

44.472

98.272

0.437
  pilG Neisseria meningitidis 44/76-A

44.221

98.272

0.435
  pilC Vibrio campbellii strain DS40M4

43.257

97.037

0.42
  pilC Vibrio cholerae strain A1552

42.145

99.012

0.417
  pilC Thermus thermophilus HB27

37.656

99.012

0.373

Multiple sequence alignment