Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   E0F39_RS10690 Genome accession   NZ_LR216060
Coordinates   1966863..1967804 (-) Length   313 a.a.
NCBI ID   WP_001864563.1    Uniprot ID   A0A0T7STT8
Organism   Streptococcus pneumoniae strain GPSC47 substr. ST315     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1961863..1972804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F39_RS10650 (SAMEA3713867_02013) comYH 1962349..1963302 (-) 954 WP_050199051.1 class I SAM-dependent methyltransferase Machinery gene
  E0F39_RS10655 - 1963363..1963957 (-) 595 Protein_2015 class I SAM-dependent methyltransferase -
  E0F39_RS10660 (SAMEA3713867_02016) comGG/cglG 1964094..1964507 (-) 414 WP_001864570.1 competence type IV pilus minor pilin ComGG Machinery gene
  E0F39_RS10665 (SAMEA3713867_02017) comGF/cglF 1964485..1964946 (-) 462 WP_001864569.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0F39_RS10670 (SAMEA3713867_02018) comGE/cglE 1964909..1965211 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  E0F39_RS10675 (SAMEA3713867_02019) comGD/cglD 1965174..1965578 (-) 405 WP_001864568.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0F39_RS10680 (SAMEA3713867_02020) comGC/cglC 1965571..1965897 (-) 327 WP_001864566.1 comG operon protein ComGC Machinery gene
  E0F39_RS10685 (SAMEA3713867_02021) comGB/cglB 1965899..1966915 (-) 1017 WP_078133877.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0F39_RS10690 (SAMEA3713867_02022) comGA/cglA/cilD 1966863..1967804 (-) 942 WP_001864563.1 competence type IV pilus ATPase ComGA Machinery gene
  E0F39_RS10695 (SAMEA3713867_02023) - 1967880..1968245 (-) 366 WP_000286415.1 DUF1033 family protein -
  E0F39_RS10700 (SAMEA3713867_02024) - 1968396..1969454 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  E0F39_RS10705 (SAMEA3713867_02025) nagA 1969617..1970768 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  E0F39_RS10710 (SAMEA3713867_02026) - 1970921..1972738 (-) 1818 WP_050199070.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35565.43 Da        Isoelectric Point: 5.6206

>NTDB_id=1126223 E0F39_RS10690 WP_001864563.1 1966863..1967804(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain GPSC47 substr. ST315]
MVQEIAQEIIRSAREKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1126223 E0F39_RS10690 WP_001864563.1 1966863..1967804(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain GPSC47 substr. ST315]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGGAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAACAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T7STT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.166

100

0.962
  comYA Streptococcus gordonii str. Challis substr. CH1

78.387

99.042

0.776
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55

Multiple sequence alignment