Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   E0F39_RS10685 Genome accession   NZ_LR216060
Coordinates   1965899..1966915 (-) Length   338 a.a.
NCBI ID   WP_078133877.1    Uniprot ID   A0AAJ5P4V7
Organism   Streptococcus pneumoniae strain GPSC47 substr. ST315     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1960899..1971915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F39_RS13080 - 1960976..1961107 (-) 132 WP_000768904.1 hypothetical protein -
  E0F39_RS10645 (SAMEA3713867_02012) - 1961108..1962298 (-) 1191 WP_001864575.1 acetate kinase -
  E0F39_RS10650 (SAMEA3713867_02013) comYH 1962349..1963302 (-) 954 WP_050199051.1 class I SAM-dependent methyltransferase Machinery gene
  E0F39_RS10655 - 1963363..1963957 (-) 595 Protein_2015 class I SAM-dependent methyltransferase -
  E0F39_RS10660 (SAMEA3713867_02016) comGG/cglG 1964094..1964507 (-) 414 WP_001864570.1 competence type IV pilus minor pilin ComGG Machinery gene
  E0F39_RS10665 (SAMEA3713867_02017) comGF/cglF 1964485..1964946 (-) 462 WP_001864569.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0F39_RS10670 (SAMEA3713867_02018) comGE/cglE 1964909..1965211 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  E0F39_RS10675 (SAMEA3713867_02019) comGD/cglD 1965174..1965578 (-) 405 WP_001864568.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0F39_RS10680 (SAMEA3713867_02020) comGC/cglC 1965571..1965897 (-) 327 WP_001864566.1 comG operon protein ComGC Machinery gene
  E0F39_RS10685 (SAMEA3713867_02021) comGB/cglB 1965899..1966915 (-) 1017 WP_078133877.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0F39_RS10690 (SAMEA3713867_02022) comGA/cglA/cilD 1966863..1967804 (-) 942 WP_001864563.1 competence type IV pilus ATPase ComGA Machinery gene
  E0F39_RS10695 (SAMEA3713867_02023) - 1967880..1968245 (-) 366 WP_000286415.1 DUF1033 family protein -
  E0F39_RS10700 (SAMEA3713867_02024) - 1968396..1969454 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  E0F39_RS10705 (SAMEA3713867_02025) nagA 1969617..1970768 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38494.57 Da        Isoelectric Point: 9.6073

>NTDB_id=1126222 E0F39_RS10685 WP_078133877.1 1965899..1966915(-) (comGB/cglB) [Streptococcus pneumoniae strain GPSC47 substr. ST315]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVIQMRAGLSQGKSFSEMMESLGFSSTI
ITQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEV
GQDLAQTLKNGREFSQMIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1126222 E0F39_RS10685 WP_078133877.1 1965899..1966915(-) (comGB/cglB) [Streptococcus pneumoniae strain GPSC47 substr. ST315]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGATCCAGATGCGTGCGGGCTTGTCTCAAGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATTTTCAAGCACCATT
ATCACTCAGTTATCCCTAGCTGAAGTTCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAGTC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGATGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae D39

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae R6

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae TIGR4

98.225

100

0.982
  comGB/cglB Streptococcus mitis SK321

94.379

100

0.944
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

57.547

94.083

0.541
  comYB Streptococcus mutans UA159

57.547

94.083

0.541
  comGB Lactococcus lactis subsp. cremoris KW2

51.198

98.817

0.506

Multiple sequence alignment