Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   E0F32_RS09965 Genome accession   NZ_LR216050
Coordinates   1836031..1836357 (-) Length   108 a.a.
NCBI ID   WP_000738625.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1831031..1841357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F32_RS09925 (SAMEA3431333_01864) rnpA 1831056..1831427 (-) 372 WP_000739247.1 ribonuclease P protein component -
  E0F32_RS12190 - 1831444..1831575 (-) 132 WP_000768904.1 hypothetical protein -
  E0F32_RS09930 (SAMEA3431333_01865) - 1831576..1832766 (-) 1191 WP_000167765.1 acetate kinase -
  E0F32_RS09935 (SAMEA3431333_01866) comYH 1832817..1833770 (-) 954 WP_000345122.1 class I SAM-dependent methyltransferase Machinery gene
  E0F32_RS09940 (SAMEA3431333_01867) - 1833831..1834418 (-) 588 WP_000679779.1 class I SAM-dependent methyltransferase -
  E0F32_RS09945 (SAMEA3431333_01868) comGG/cglG 1834521..1834967 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  E0F32_RS09950 (SAMEA3431333_01869) comGF/cglF 1834945..1835406 (-) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0F32_RS09955 (SAMEA3431333_01870) comGE/cglE 1835369..1835671 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  E0F32_RS09960 (SAMEA3431333_01871) comGD/cglD 1835634..1836038 (-) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0F32_RS09965 (SAMEA3431333_01872) comGC/cglC 1836031..1836357 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  E0F32_RS09970 (SAMEA3431333_01873) comGB/cglB 1836359..1837375 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0F32_RS09975 (SAMEA3431333_01874) comGA/cglA/cilD 1837323..1838264 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  E0F32_RS09980 (SAMEA3431333_01875) - 1838339..1838704 (-) 366 WP_000286415.1 DUF1033 family protein -
  E0F32_RS09985 (SAMEA3431333_01876) - 1838855..1839913 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  E0F32_RS09990 (SAMEA3431333_01877) nagA 1840076..1841227 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 108 a.a.        Molecular weight: 12143.28 Da        Isoelectric Point: 10.0055

>NTDB_id=1126147 E0F32_RS09965 WP_000738625.1 1836031..1836357(-) (comGC/cglC) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKEAVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQG
DGRITEEQVKAYNEYYTKNGGANRKVND

Nucleotide


Download         Length: 327 bp        

>NTDB_id=1126147 E0F32_RS09965 WP_000738625.1 1836031..1836357(-) (comGC/cglC) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
ATGAAAAAAATGATGACATTCTTGAAAAAAGCTAAGGTTAAAGCTTTTACATTGGTGGAGATGTTAGTAGTCTTGCTGAT
TATCAGCGTGCTTTTCTTGCTCTTTGTACCTAATCTGACCAAGCAAAAAGAAGCAGTCAATGACAAAGGAAAAGCAGCTG
TTGTTAAGGTGGTGGAAAGCCAGGCAGAACTTTATAGCTTGGATAAAAATGAAGATGCTACTCTGAGCAAGTTACAAGGA
GATGGGCGCATCACGGAAGAACAGGTTAAAGCTTATAATGAATACTATACTAAAAATGGAGGGGCAAATCGTAAAGTCAA
TGATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus mitis SK321

95.37

100

0.954
  comGC/cglC Streptococcus pneumoniae Rx1

92.593

100

0.926
  comGC/cglC Streptococcus pneumoniae D39

92.593

100

0.926
  comGC/cglC Streptococcus pneumoniae R6

92.593

100

0.926
  comGC/cglC Streptococcus pneumoniae TIGR4

92.593

100

0.926
  comGC/cglC Streptococcus mitis NCTC 12261

92.857

90.741

0.843
  comYC Streptococcus gordonii str. Challis substr. CH1

67.961

95.37

0.648
  comYC Streptococcus mutans UA140

62.745

94.444

0.593
  comYC Streptococcus mutans UA159

62.745

94.444

0.593
  comGC Lactococcus lactis subsp. cremoris KW2

56.863

94.444

0.537
  comYC Streptococcus suis isolate S10

64.773

81.481

0.528
  comGC Staphylococcus aureus MW2

47.674

79.63

0.38
  comGC Staphylococcus aureus N315

47.674

79.63

0.38

Multiple sequence alignment