Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   E0F32_RS09975 Genome accession   NZ_LR216050
Coordinates   1837323..1838264 (-) Length   313 a.a.
NCBI ID   WP_000249555.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1832323..1843264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F32_RS09935 (SAMEA3431333_01866) comYH 1832817..1833770 (-) 954 WP_000345122.1 class I SAM-dependent methyltransferase Machinery gene
  E0F32_RS09940 (SAMEA3431333_01867) - 1833831..1834418 (-) 588 WP_000679779.1 class I SAM-dependent methyltransferase -
  E0F32_RS09945 (SAMEA3431333_01868) comGG/cglG 1834521..1834967 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  E0F32_RS09950 (SAMEA3431333_01869) comGF/cglF 1834945..1835406 (-) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0F32_RS09955 (SAMEA3431333_01870) comGE/cglE 1835369..1835671 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  E0F32_RS09960 (SAMEA3431333_01871) comGD/cglD 1835634..1836038 (-) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0F32_RS09965 (SAMEA3431333_01872) comGC/cglC 1836031..1836357 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  E0F32_RS09970 (SAMEA3431333_01873) comGB/cglB 1836359..1837375 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0F32_RS09975 (SAMEA3431333_01874) comGA/cglA/cilD 1837323..1838264 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  E0F32_RS09980 (SAMEA3431333_01875) - 1838339..1838704 (-) 366 WP_000286415.1 DUF1033 family protein -
  E0F32_RS09985 (SAMEA3431333_01876) - 1838855..1839913 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  E0F32_RS09990 (SAMEA3431333_01877) nagA 1840076..1841227 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  E0F32_RS09995 (SAMEA3431333_01878) - 1841380..1843197 (-) 1818 WP_001220846.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35561.49 Da        Isoelectric Point: 6.0083

>NTDB_id=1126149 E0F32_RS09975 WP_000249555.1 1837323..1838264(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1126149 E0F32_RS09975 WP_000249555.1 1837323..1838264(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.722

100

0.987
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.125

100

0.971
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment