Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   E0F32_RS09970 Genome accession   NZ_LR216050
Coordinates   1836359..1837375 (-) Length   338 a.a.
NCBI ID   WP_074017570.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1831359..1842375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F32_RS12190 - 1831444..1831575 (-) 132 WP_000768904.1 hypothetical protein -
  E0F32_RS09930 (SAMEA3431333_01865) - 1831576..1832766 (-) 1191 WP_000167765.1 acetate kinase -
  E0F32_RS09935 (SAMEA3431333_01866) comYH 1832817..1833770 (-) 954 WP_000345122.1 class I SAM-dependent methyltransferase Machinery gene
  E0F32_RS09940 (SAMEA3431333_01867) - 1833831..1834418 (-) 588 WP_000679779.1 class I SAM-dependent methyltransferase -
  E0F32_RS09945 (SAMEA3431333_01868) comGG/cglG 1834521..1834967 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  E0F32_RS09950 (SAMEA3431333_01869) comGF/cglF 1834945..1835406 (-) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0F32_RS09955 (SAMEA3431333_01870) comGE/cglE 1835369..1835671 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  E0F32_RS09960 (SAMEA3431333_01871) comGD/cglD 1835634..1836038 (-) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0F32_RS09965 (SAMEA3431333_01872) comGC/cglC 1836031..1836357 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  E0F32_RS09970 (SAMEA3431333_01873) comGB/cglB 1836359..1837375 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0F32_RS09975 (SAMEA3431333_01874) comGA/cglA/cilD 1837323..1838264 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  E0F32_RS09980 (SAMEA3431333_01875) - 1838339..1838704 (-) 366 WP_000286415.1 DUF1033 family protein -
  E0F32_RS09985 (SAMEA3431333_01876) - 1838855..1839913 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  E0F32_RS09990 (SAMEA3431333_01877) nagA 1840076..1841227 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38482.48 Da        Isoelectric Point: 9.6162

>NTDB_id=1126148 E0F32_RS09970 WP_074017570.1 1836359..1837375(-) (comGB/cglB) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1126148 E0F32_RS09970 WP_074017570.1 1836359..1837375(-) (comGB/cglB) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae D39

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae R6

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae TIGR4

98.521

100

0.985
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment