Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL305_RS02570 Genome accession   NZ_LR134528
Coordinates   440603..441733 (+) Length   376 a.a.
NCBI ID   WP_061726970.1    Uniprot ID   -
Organism   Neisseria meningitidis strain NCTC12163     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 435603..446733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL305_RS14480 - 436432..436538 (-) 107 Protein_431 IS5/IS1182 family transposase -
  EL305_RS02555 (NCTC12163_00440) lepB 436636..437655 (-) 1020 WP_002236844.1 signal peptidase I -
  EL305_RS02560 (NCTC12163_00441) lepA 437798..439591 (-) 1794 WP_002236845.1 translation elongation factor 4 -
  EL305_RS02565 (NCTC12163_00442) - 439734..440435 (-) 702 WP_002222734.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  EL305_RS02570 (NCTC12163_00443) pilU 440603..441733 (+) 1131 WP_061726970.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL305_RS02575 (NCTC12163_00444) - 441767..442744 (+) 978 WP_002236848.1 DNA polymerase III subunit delta' -
  EL305_RS02580 (NCTC12163_00445) - 442748..443098 (+) 351 WP_002214009.1 PilZ domain-containing protein -
  EL305_RS02585 (NCTC12163_00446) - 443103..443882 (+) 780 WP_002214008.1 TatD family hydrolase -
  EL305_RS02590 (NCTC12163_00447) - 443908..444123 (+) 216 WP_002225429.1 DUF333 domain-containing protein -
  EL305_RS02595 (NCTC12163_00448) grxD 444224..444535 (+) 312 WP_002214004.1 Grx4 family monothiol glutaredoxin -
  EL305_RS02605 (NCTC12163_00450) upp 444641..445267 (+) 627 WP_002225428.1 uracil phosphoribosyltransferase -
  EL305_RS02610 (NCTC12163_00451) - 445290..445610 (+) 321 WP_002214001.1 DUF2322 family protein -
  EL305_RS02615 (NCTC12163_00452) - 445861..446283 (+) 423 WP_002226495.1 protein Gly1ORF1 -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42273.40 Da        Isoelectric Point: 6.2894

>NTDB_id=1124016 EL305_RS02570 WP_061726970.1 440603..441733(+) (pilU) [Neisseria meningitidis strain NCTC12163]
MFTDENMTEKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1124016 EL305_RS02570 WP_061726970.1 440603..441733(+) (pilU) [Neisseria meningitidis strain NCTC12163]
ATGTTTACCGATGAAAATATGACCGAAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTTGTGACGACCCATTTCCCGCCCGCAATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACGGCGTTGGTATTCCG
TGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCGCCGGATGTCATCCTTATCGGCG
AAATCCGCGACCGCGAAACAATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGATCAACACCTTTACCAATTGTATGAAAAAGGCGATATTTCCCTGCAAGAAGCATTGAA
AAATGCCGATTCCGCACACGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCCGCGCGCAAAGTTCCAGCCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.375

93.617

0.556
  pilU Acinetobacter baylyi ADP1

57.434

91.223

0.524
  pilU Vibrio cholerae strain A1552

54.366

94.415

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Acinetobacter baylyi ADP1

42.727

87.766

0.375
  pilT Pseudomonas aeruginosa PAK

42.683

87.234

0.372
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.768

87.234

0.364

Multiple sequence alignment