Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL323_RS07045 Genome accession   NZ_LR134526
Coordinates   1201089..1202219 (-) Length   376 a.a.
NCBI ID   WP_002232075.1    Uniprot ID   C6S635
Organism   Neisseria meningitidis strain NCTC10026     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1196089..1207219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL323_RS07000 (NCTC10026_01163) - 1196539..1196961 (-) 423 WP_002242455.1 protein Gly1ORF1 -
  EL323_RS07005 (NCTC10026_01164) - 1197212..1197532 (-) 321 WP_002214001.1 DUF2322 family protein -
  EL323_RS07010 (NCTC10026_01165) upp 1197555..1198181 (-) 627 WP_002214002.1 uracil phosphoribosyltransferase -
  EL323_RS07020 (NCTC10026_01167) grxD 1198287..1198598 (-) 312 WP_002214004.1 Grx4 family monothiol glutaredoxin -
  EL323_RS07025 (NCTC10026_01168) - 1198699..1198914 (-) 216 WP_002243409.1 DUF333 domain-containing protein -
  EL323_RS07030 (NCTC10026_01169) - 1198940..1199719 (-) 780 WP_080542291.1 TatD family hydrolase -
  EL323_RS07035 (NCTC10026_01170) - 1199724..1200074 (-) 351 WP_002214009.1 PilZ domain-containing protein -
  EL323_RS07040 (NCTC10026_01171) - 1200078..1201055 (-) 978 WP_002236848.1 DNA polymerase III subunit delta' -
  EL323_RS07045 (NCTC10026_01172) pilU 1201089..1202219 (-) 1131 WP_002232075.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL323_RS07050 (NCTC10026_01173) - 1202387..1203088 (+) 702 WP_002224607.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  EL323_RS07055 (NCTC10026_01174) lepA 1203230..1205023 (+) 1794 WP_080542292.1 translation elongation factor 4 -
  EL323_RS07060 (NCTC10026_01175) lepB 1205166..1206185 (+) 1020 WP_080542293.1 signal peptidase I -
  EL323_RS15470 - 1206283..1206389 (+) 107 Protein_1157 IS5/IS1182 family transposase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42215.32 Da        Isoelectric Point: 6.2885

>NTDB_id=1123976 EL323_RS07045 WP_002232075.1 1201089..1202219(-) (pilU) [Neisseria meningitidis strain NCTC10026]
MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1123976 EL323_RS07045 WP_002232075.1 1201089..1202219(-) (pilU) [Neisseria meningitidis strain NCTC10026]
ATGTTTACCGATGAAAATATGACCGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACCAAAACAAAGGTTCCGA
CCTGTTCGTGACGACTCATTTCCCGCCCGCAATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGTCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACAGCGTTGGTATTCCG
TGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCGCCGGATGTCATCCTTATCGGCG
AAATCCGCGACCGCGAAACAATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGATCAACACCTTTACCAATTGTATGAAAAAGGCGATATTTCCCTGCAAGAAGCATTGAA
AAATGCCGATTCCGCACACGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCCGCGCGCAAAGTTCCAGCCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6S635

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.375

93.617

0.556
  pilU Acinetobacter baylyi ADP1

57.143

91.223

0.521
  pilU Vibrio cholerae strain A1552

54.366

94.415

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Acinetobacter baylyi ADP1

43.03

87.766

0.378
  pilT Legionella pneumophila strain Lp02

43.519

86.17

0.375
  pilT Legionella pneumophila strain ERS1305867

43.519

86.17

0.375
  pilT Pseudomonas aeruginosa PAK

42.378

87.234

0.37
  pilT Acinetobacter baumannii strain A118

42.593

86.17

0.367
  pilT Acinetobacter nosocomialis M2

42.593

86.17

0.367
  pilT Acinetobacter baumannii D1279779

42.593

86.17

0.367
  pilT Neisseria meningitidis 8013

40.597

89.096

0.362
  pilT Pseudomonas stutzeri DSM 10701

41.463

87.234

0.362

Multiple sequence alignment