Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL276_RS07930 Genome accession   NZ_LR134516
Coordinates   1718169..1719299 (+) Length   376 a.a.
NCBI ID   WP_085391155.1    Uniprot ID   A0A1X3CGU6
Organism   Neisseria animaloris strain NCTC12227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1713169..1724299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL276_RS07915 (NCTC12227_01609) - 1714022..1714804 (+) 783 WP_159070107.1 siderophore ferric iron reductase -
  EL276_RS07920 (NCTC12227_01610) - 1714791..1715567 (+) 777 WP_085391153.1 ABC transporter ATP-binding protein -
  EL276_RS07925 (NCTC12227_01613) - 1717275..1717994 (-) 720 WP_085391154.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  EL276_RS07930 (NCTC12227_01614) pilU 1718169..1719299 (+) 1131 WP_085391155.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL276_RS07935 (NCTC12227_01615) ubiB 1719376..1720887 (-) 1512 WP_107928710.1 ubiquinone biosynthesis regulatory protein kinase UbiB -
  EL276_RS07940 (NCTC12227_01616) ccoP 1721525..1722475 (-) 951 Protein_1551 cytochrome-c oxidase, cbb3-type subunit III -
  EL276_RS07945 (NCTC12227_01617) - 1722506..1722670 (-) 165 WP_107928709.1 cbb3-type cytochrome oxidase subunit 3 -
  EL276_RS07950 (NCTC12227_01618) ccoO 1722675..1723286 (-) 612 WP_085391159.1 cytochrome-c oxidase, cbb3-type subunit II -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42177.18 Da        Isoelectric Point: 5.4764

>NTDB_id=1123731 EL276_RS07930 WP_085391155.1 1718169..1719299(+) (pilU) [Neisseria animaloris strain NCTC12227]
MEQNNTLSAKEEIYSWLRTLNKYKGSDLFITANFPPAMKLDGKITRITDTPLTPERCMEIAFAIMSPKQSEEFMATNECN
FAISTPDTSRFRVNAMVQRGATALVFRTITSNIPKFETLNLPPILQQVAMQKRGLVIFVGGTGSGKSTSLASMIDYRNEN
SQDHIITIEDPIEFVHEHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYAIAFSETGHLCMATLHA
NSTNQALDRIINFFPEERRVQLLTDLSLNLQAFISQRLIPREGGKGRVAAVEVLLNSPLVAELIHNGDVHSIKEVMKKSQ
ALGMQTFDQALYELYEGGLISFQDAIKNADSANDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1123731 EL276_RS07930 WP_085391155.1 1718169..1719299(+) (pilU) [Neisseria animaloris strain NCTC12227]
ATGGAACAAAATAATACTCTCTCCGCAAAAGAAGAAATTTATTCATGGCTGCGTACATTAAATAAATATAAAGGCTCCGA
CCTTTTTATTACCGCCAACTTTCCGCCTGCAATGAAACTTGACGGCAAAATTACCCGCATTACTGATACACCCTTAACTC
CGGAACGCTGTATGGAGATTGCTTTTGCAATTATGTCTCCCAAGCAGTCGGAAGAATTTATGGCAACCAATGAATGCAAC
TTTGCTATCAGCACTCCCGACACCAGCCGCTTCAGGGTTAATGCTATGGTGCAACGCGGTGCTACGGCATTAGTTTTCCG
CACCATTACCAGCAACATTCCCAAATTTGAAACACTCAATTTACCGCCCATACTGCAACAAGTCGCCATGCAAAAGCGGG
GATTAGTCATTTTTGTCGGAGGAACAGGCTCCGGCAAATCTACTTCTCTCGCATCGATGATTGATTACCGCAACGAAAAC
AGCCAAGATCATATCATCACAATTGAGGATCCCATTGAATTTGTGCATGAACATAAAAATTGCATCATCACTCAGCGCGA
AGTCGGTGTTGATACCGAAAACTGGTTTGCAGCCCTTAAAAATACACTTCGCCAAGCACCGGATGTAATTCTGATCGGCG
AGATTCGTGATCGTGAAACAATGGATTATGCTATTGCTTTCTCAGAAACAGGCCACTTGTGTATGGCTACTCTGCATGCA
AACAGTACAAACCAAGCACTTGACCGCATTATTAACTTTTTTCCGGAAGAACGTCGTGTACAATTGCTAACTGACTTGTC
ATTAAATTTACAGGCTTTTATTTCACAGCGTTTGATTCCGAGAGAAGGAGGTAAAGGTCGTGTCGCGGCGGTTGAAGTAT
TACTCAACTCTCCTTTAGTAGCTGAATTAATTCATAATGGTGACGTGCACTCTATTAAAGAAGTAATGAAAAAATCACAA
GCTCTCGGCATGCAGACTTTTGACCAAGCTTTATATGAGCTATATGAAGGAGGTTTGATCAGCTTCCAAGACGCGATTAA
AAATGCAGATTCCGCCAATGATTTACGTTTGGATATCCAATTACGCAGTCGCCGCGCCCAAAATGCAGGCCCTGATTTAG
AATTGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X3CGU6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.943

93.617

0.561
  pilU Acinetobacter baylyi ADP1

57.637

92.287

0.532
  pilU Vibrio cholerae strain A1552

53.482

95.479

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.604

89.894

0.383
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Pseudomonas aeruginosa PAK

42.683

87.234

0.372
  pilT Acinetobacter baylyi ADP1

42.683

87.234

0.372
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.463

87.234

0.362

Multiple sequence alignment