Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL216_RS09170 Genome accession   NZ_LR134440
Coordinates   1953423..1954553 (-) Length   376 a.a.
NCBI ID   WP_085391155.1    Uniprot ID   A0A1X3CGU6
Organism   Neisseria animaloris strain NCTC12228     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1948423..1959553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL216_RS09150 (NCTC12228_01846) ccoO 1949437..1950048 (+) 612 WP_085391159.1 cytochrome-c oxidase, cbb3-type subunit II -
  EL216_RS09155 (NCTC12228_01847) - 1950053..1950217 (+) 165 WP_085391158.1 CcoQ/FixQ family Cbb3-type cytochrome c oxidase assembly chaperone -
  EL216_RS09160 (NCTC12228_01848) ccoP 1950248..1951198 (+) 951 Protein_1802 cytochrome-c oxidase, cbb3-type subunit III -
  EL216_RS09165 (NCTC12228_01849) ubiB 1951835..1953346 (+) 1512 WP_085391156.1 ubiquinone biosynthesis regulatory protein kinase UbiB -
  EL216_RS09170 (NCTC12228_01850) pilU 1953423..1954553 (-) 1131 WP_085391155.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL216_RS09175 (NCTC12228_01851) - 1954728..1955447 (+) 720 WP_085391154.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  EL216_RS09180 (NCTC12228_01852) - 1956022..1956228 (+) 207 WP_197720427.1 hypothetical protein -
  EL216_RS09185 (NCTC12228_01854) - 1957156..1957932 (-) 777 WP_085391153.1 ABC transporter ATP-binding protein -
  EL216_RS09190 (NCTC12228_01855) - 1957919..1958701 (-) 783 WP_158087742.1 siderophore ferric iron reductase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42177.18 Da        Isoelectric Point: 5.4764

>NTDB_id=1122872 EL216_RS09170 WP_085391155.1 1953423..1954553(-) (pilU) [Neisseria animaloris strain NCTC12228]
MEQNNTLSAKEEIYSWLRTLNKYKGSDLFITANFPPAMKLDGKITRITDTPLTPERCMEIAFAIMSPKQSEEFMATNECN
FAISTPDTSRFRVNAMVQRGATALVFRTITSNIPKFETLNLPPILQQVAMQKRGLVIFVGGTGSGKSTSLASMIDYRNEN
SQDHIITIEDPIEFVHEHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYAIAFSETGHLCMATLHA
NSTNQALDRIINFFPEERRVQLLTDLSLNLQAFISQRLIPREGGKGRVAAVEVLLNSPLVAELIHNGDVHSIKEVMKKSQ
ALGMQTFDQALYELYEGGLISFQDAIKNADSANDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1122872 EL216_RS09170 WP_085391155.1 1953423..1954553(-) (pilU) [Neisseria animaloris strain NCTC12228]
ATGGAACAAAATAATACTCTCTCCGCAAAAGAAGAAATTTATTCATGGCTGCGTACATTAAATAAATATAAAGGCTCCGA
CCTTTTTATTACCGCCAACTTTCCGCCTGCAATGAAACTTGACGGCAAAATTACCCGCATTACTGATACACCCTTAACTC
CGGAACGCTGTATGGAGATTGCTTTTGCAATTATGTCTCCCAAGCAGTCGGAAGAATTTATGGCAACCAATGAATGCAAC
TTTGCTATCAGCACTCCCGACACCAGCCGCTTCAGGGTTAATGCTATGGTGCAACGCGGTGCTACGGCATTAGTTTTCCG
CACCATTACCAGCAACATTCCCAAATTTGAAACACTCAATTTACCGCCCATATTGCAACAAGTCGCCATGCAAAAGCGGG
GATTAGTCATTTTTGTCGGAGGAACAGGCTCCGGCAAATCTACTTCTCTCGCATCGATGATTGATTACCGCAATGAAAAC
AGCCAAGATCATATCATCACAATTGAGGATCCCATTGAATTTGTGCATGAACATAAAAATTGCATCATCACTCAGCGCGA
AGTCGGTGTTGATACCGAAAACTGGTTTGCAGCCCTTAAAAATACACTTCGCCAAGCACCGGATGTGATTCTGATTGGCG
AGATTCGTGATCGTGAAACAATGGATTATGCTATTGCTTTCTCAGAAACAGGCCACTTGTGTATGGCTACTCTGCATGCA
AACAGCACAAACCAAGCACTTGACCGCATTATTAACTTTTTTCCGGAAGAACGTCGTGTACAATTACTAACTGACTTGTC
ATTAAATTTACAGGCTTTTATTTCACAGCGTTTGATTCCGAGAGAAGGAGGTAAAGGTCGTGTCGCGGCGGTTGAAGTAT
TACTCAACTCTCCTTTAGTAGCTGAATTAATTCATAATGGTGACGTGCACTCTATTAAAGAAGTAATGAAAAAATCACAA
GCTCTCGGCATGCAGACTTTTGACCAAGCTTTATATGAGCTATATGAAGGAGGTTTGATCAGCTTCCAAGACGCGATTAA
AAATGCAGATTCCGCCAATGATTTACGTTTGGATATCCAATTACGCAGTCGCCGCGCCCAAAATGCAGGCCCTGATTTAG
AATTGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X3CGU6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.943

93.617

0.561
  pilU Acinetobacter baylyi ADP1

57.637

92.287

0.532
  pilU Vibrio cholerae strain A1552

53.482

95.479

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.604

89.894

0.383
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Pseudomonas aeruginosa PAK

42.683

87.234

0.372
  pilT Acinetobacter baylyi ADP1

42.683

87.234

0.372
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.463

87.234

0.362

Multiple sequence alignment