Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   EL140_RS00825 Genome accession   NZ_LR134336
Coordinates   152489..153430 (+) Length   313 a.a.
NCBI ID   WP_000249583.1    Uniprot ID   A0A1X1HB40
Organism   Streptococcus oralis ATCC 35037 strain NCTC 11427     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 147489..158430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL140_RS00805 (NCTC11427_00154) tgt 147519..148661 (+) 1143 WP_001285237.1 tRNA guanosine(34) transglycosylase Tgt -
  EL140_RS00810 (NCTC11427_00155) - 148795..150612 (+) 1818 WP_001220886.1 acyltransferase family protein -
  EL140_RS00815 (NCTC11427_00156) nagA 150753..151904 (+) 1152 WP_001134442.1 N-acetylglucosamine-6-phosphate deacetylase -
  EL140_RS00820 (NCTC11427_00157) - 152062..152427 (+) 366 WP_000286399.1 DUF1033 family protein -
  EL140_RS00825 (NCTC11427_00158) comGA/cglA/cilD 152489..153430 (+) 942 WP_000249583.1 competence type IV pilus ATPase ComGA Machinery gene
  EL140_RS00830 (NCTC11427_00159) comGB/cglB 153378..154394 (+) 1017 WP_002875106.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL140_RS00835 (NCTC11427_00160) comGC/cglC 154396..154719 (+) 324 WP_000735794.1 competence type IV pilus major pilin ComGC Machinery gene
  EL140_RS00840 (NCTC11427_00161) comGD/cglD 154682..155116 (+) 435 WP_001180060.1 competence type IV pilus minor pilin ComGD Machinery gene
  EL140_RS00845 (NCTC11427_00162) comGE/cglE 155079..155381 (+) 303 WP_000413369.1 competence type IV pilus minor pilin ComGE Machinery gene
  EL140_RS00850 (NCTC11427_00163) comGF/cglF 155344..155805 (+) 462 WP_002875275.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL140_RS00855 (NCTC11427_00164) comGG/cglG 155783..156196 (+) 414 WP_000265647.1 competence type IV pilus minor pilin ComGG Machinery gene
  EL140_RS00860 (NCTC11427_00165) - 156229..156816 (+) 588 WP_000679790.1 class I SAM-dependent methyltransferase -
  EL140_RS00865 (NCTC11427_00166) comYH 156877..157830 (+) 954 WP_000345144.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35539.43 Da        Isoelectric Point: 5.4408

>NTDB_id=1121812 EL140_RS00825 WP_000249583.1 152489..153430(+) (comGA/cglA/cilD) [Streptococcus oralis ATCC 35037 strain NCTC 11427]
MVQEIAQKIIATAKEKKAQDIYFIPKEKSYELHMRVGDERCLVDSYEFDVLAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
HGEKKSSLRLSTVGDYRGHESLVIRLLHDEEQDLNFWFQDMNELGEQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQEDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQIEQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1121812 EL140_RS00825 WP_000249583.1 152489..153430(+) (comGA/cglA/cilD) [Streptococcus oralis ATCC 35037 strain NCTC 11427]
ATGGTACAAGAAATTGCACAGAAAATTATTGCTACTGCGAAAGAAAAGAAGGCTCAGGATATCTATTTCATTCCCAAGGA
AAAGTCCTACGAGCTTCACATGCGGGTTGGAGACGAGCGGTGTCTCGTTGACTCCTATGAGTTTGATGTTTTAGCTGCTG
TGATTAGTCATTTTAAGTTTGTGGCGGGTATGAACGTAGGAGAGAAGAGGCGCAGTCAGCTGGGTTCTTGCGACTATCAG
CATGGGGAGAAGAAGTCTTCTCTGCGTTTGTCTACCGTAGGAGATTATCGGGGGCATGAGAGTTTGGTCATTCGTTTGTT
GCACGATGAGGAGCAGGATCTGAATTTCTGGTTTCAGGATATGAACGAACTGGGTGAGCAGTACAGGCAACGGGGACTCT
ATCTATTTGCAGGTCCAGTCGGAAGTGGCAAGACGACCTTGATGCACGAATTGGCCAAGTCTCTGTTTAAAGGGCAACAG
GTTATGTCCATTGAAGATCCTGTCGAGATCAAGCAGGAAGACATGCTCCAGTTGCAGTTGAATGAAGCGATTGGCTTGAC
CTATGAAAATCTGATCAAACTGTCTCTCCGGCATCGTCCGGACCTCTTGATTATTGGTGAAATTCGGGATAGCGAGACAG
CGCGTGCAGTGGTCAGAGCCAGTTTGACAGGGGCGACAGTTTTTTCAACCATTCATGCCAAGAGTATCCGAGGTGTTTAT
GAACGCCTTCTGGAGTTGGGTGTGACGGAGGAGGAATTAGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATTGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAGGAACACCAGCCAACTAGCTGGAATGAACAGATTGAGCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X1HB40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

89.137

100

0.891
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae D39

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae R6

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

88.179

100

0.882
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.042

0.597
  comGA Lactococcus lactis subsp. cremoris KW2

55.449

99.681

0.553
  comGA Latilactobacillus sakei subsp. sakei 23K

43.123

85.942

0.371

Multiple sequence alignment