Detailed information NTG1121817

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: EL140_RS00850
• Genome accession: NZ_LR134336
• Coordinates: 155344..155805 (+)
• Length: 153 a.a.
• NCBI ID: WP_002875275.1
• Uniprot ID: A0A1X1HB00
• Organism: Streptococcus oralis ATCC 35037 strain NCTC 11427
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 150344..160805

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL140_RS00815 (NCTC11427_00156)	nagA	150753..151904 (+)	1152	WP_001134442.1	N-acetylglucosamine-6-phosphate deacetylase	-
EL140_RS00820 (NCTC11427_00157)	-	152062..152427 (+)	366	WP_000286399.1	DUF1033 family protein	-
EL140_RS00825 (NCTC11427_00158)	comGA/cglA/cilD	152489..153430 (+)	942	WP_000249583.1	competence type IV pilus ATPase ComGA	Machinery gene
EL140_RS00830 (NCTC11427_00159)	comGB/cglB	153378..154394 (+)	1017	WP_002875106.1	competence type IV pilus assembly protein ComGB	Machinery gene
EL140_RS00835 (NCTC11427_00160)	comGC/cglC	154396..154719 (+)	324	WP_000735794.1	competence type IV pilus major pilin ComGC	Machinery gene
EL140_RS00840 (NCTC11427_00161)	comGD/cglD	154682..155116 (+)	435	WP_001180060.1	competence type IV pilus minor pilin ComGD	Machinery gene
EL140_RS00845 (NCTC11427_00162)	comGE/cglE	155079..155381 (+)	303	WP_000413369.1	competence type IV pilus minor pilin ComGE	Machinery gene
EL140_RS00850 (NCTC11427_00163)	comGF/cglF	155344..155805 (+)	462	WP_002875275.1	competence type IV pilus minor pilin ComGF	Machinery gene
EL140_RS00855 (NCTC11427_00164)	comGG/cglG	155783..156196 (+)	414	WP_000265647.1	competence type IV pilus minor pilin ComGG	Machinery gene
EL140_RS00860 (NCTC11427_00165)	-	156229..156816 (+)	588	WP_000679790.1	class I SAM-dependent methyltransferase	-
EL140_RS00865 (NCTC11427_00166)	comYH	156877..157830 (+)	954	WP_000345144.1	class I SAM-dependent methyltransferase	Machinery gene
EL140_RS00870 (NCTC11427_00167)	-	157882..159072 (+)	1191	WP_000167789.1	acetate kinase	-
EL140_RS00875 (NCTC11427_00168)	rnpA	159222..159593 (+)	372	WP_000748120.1	ribonuclease P protein component	-
EL140_RS00880 (NCTC11427_00169)	-	159568..160395 (+)	828	WP_000728097.1	membrane protein insertase YidC	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17787.41 Da
• Isoelectric Point: 9.6234

>NTDB_id=1121817 EL140_RS00850 WP_002875275.1 155344..155805(+) (comGF/cglF) [Streptococcus oralis ATCC 35037 strain NCTC 11427]
MVQRSCSTLKSSKVRAFTLLESLIALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELERSQFEKVEGN
RLYLKQDGKDISIGKSKSDDFRKTDTSGRGYQPMVYGLKSAQITEENQLVRFRFQFQKGLEREFIYRVEKTKS

Nucleotide

• Length: 462 bp

>NTDB_id=1121817 EL140_RS00850 WP_002875275.1 155344..155805(+) (comGF/cglF) [Streptococcus oralis ATCC 35037 strain NCTC 11427]
ATGGTTCAGAGAAGTTGCTCGACCTTAAAGAGCAGTAAGGTAAGAGCGTTCACTCTATTAGAATCTCTGATTGCCCTTAT
CGTCATTAGCGGAGGCTTGCTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGTTACCAGCAGCAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAACTGGAGGCAGAGTTAGAGCGTTCGCAGTTTGAAAAGGTAGAAGGCAAT
CGCCTCTATTTAAAACAAGATGGTAAGGATATCTCTATAGGGAAGTCTAAATCGGATGATTTTCGAAAAACGGATACCAG
CGGACGGGGCTATCAGCCGATGGTTTATGGACTCAAATCAGCTCAAATTACAGAGGAAAATCAATTGGTTCGCTTTCGTT
TCCAATTTCAAAAGGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAACAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

3D structure

• AlphaFold DB: A0A1X1HB00

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis SK321	90.196	100	0.902
comGF/cglF	Streptococcus mitis NCTC 12261	88.889	100	0.889
comGF/cglF	Streptococcus pneumoniae Rx1	87.582	100	0.876
comGF/cglF	Streptococcus pneumoniae D39	87.582	100	0.876
comGF/cglF	Streptococcus pneumoniae R6	87.582	100	0.876
comGF/cglF	Streptococcus pneumoniae TIGR4	87.582	100	0.876
comGF	Lactococcus lactis subsp. cremoris KW2	41.429	91.503	0.379