Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   EL022_RS12725 Genome accession   NZ_LR134173
Coordinates   2995042..2995473 (+) Length   143 a.a.
NCBI ID   WP_028380216.1    Uniprot ID   A0A0W0S930
Organism   Legionella cherrii strain NCTC11976     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2990042..3000473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL022_RS12715 (NCTC11976_02648) letS 2990686..2993418 (+) 2733 WP_028380218.1 ATP-binding protein Regulator
  EL022_RS12720 (NCTC11976_02649) - 2993495..2994733 (+) 1239 WP_028380217.1 6-phosphofructokinase -
  EL022_RS12725 (NCTC11976_02650) pilA2 2995042..2995473 (+) 432 WP_028380216.1 pilin Machinery gene
  EL022_RS12730 (NCTC11976_02651) - 2995540..2995869 (+) 330 WP_028380215.1 BolA family protein -
  EL022_RS12735 (NCTC11976_02652) - 2996076..2996417 (+) 342 WP_028380214.1 hypothetical protein -
  EL022_RS12740 (NCTC11976_02653) - 2996663..2998069 (+) 1407 WP_028380213.1 APC family permease -
  EL022_RS12745 (NCTC11976_02654) - 2998223..2998402 (-) 180 WP_028380212.1 Trm112 family protein -
  EL022_RS12750 (NCTC11976_02655) tsaB 2998420..2999070 (-) 651 WP_035900420.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14809.99 Da        Isoelectric Point: 4.8016

>NTDB_id=1118783 EL022_RS12725 WP_028380216.1 2995042..2995473(+) (pilA2) [Legionella cherrii strain NCTC11976]
MKQKGFTLIELMIVVAIVGILAAIAIPAYQDYVVRARVTEGLSLASSAQTTISENATTGANALDLGWSQPPATANVQSVV
VTPATGVITVTYTALANNVVLTLTPSSNGQPLVAGTPPTNPITWTCAVQNAADDRYVPANCRI

Nucleotide


Download         Length: 432 bp        

>NTDB_id=1118783 EL022_RS12725 WP_028380216.1 2995042..2995473(+) (pilA2) [Legionella cherrii strain NCTC11976]
ATGAAACAAAAAGGCTTTACGTTAATTGAGTTGATGATAGTGGTGGCAATCGTTGGTATCCTTGCCGCAATTGCTATTCC
AGCTTATCAGGATTACGTTGTTAGAGCTCGTGTAACCGAAGGATTGAGTCTTGCTTCTTCAGCACAGACTACAATTTCTG
AAAATGCTACAACGGGTGCTAATGCTTTAGACTTGGGCTGGTCTCAACCCCCAGCAACAGCTAACGTGCAAAGTGTTGTA
GTTACTCCAGCTACTGGCGTAATTACAGTGACCTATACTGCACTAGCAAATAATGTGGTCCTTACATTGACTCCATCATC
TAATGGACAACCATTGGTTGCAGGAACACCGCCTACAAATCCAATTACCTGGACTTGTGCAGTACAAAATGCTGCTGATG
ATAGATATGTTCCTGCAAACTGTAGAATCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(34-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0S930

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

53.691

100

0.559
  pilA Ralstonia pseudosolanacearum GMI1000

50.955

100

0.559
  pilA2 Legionella pneumophila str. Paris

53.02

100

0.552
  comP Acinetobacter baylyi ADP1

45.161

100

0.49
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.877

100

0.455
  pilA/pilA1 Eikenella corrodens VA1

39.752

100

0.448
  pilE Neisseria gonorrhoeae MS11

35.802

100

0.406
  pilA Acinetobacter baumannii strain A118

37.584

100

0.392
  pilA Pseudomonas aeruginosa PAK

35.484

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

33.333

100

0.385
  pilA Vibrio campbellii strain DS40M4

36.054

100

0.371
  pilA/pilAII Pseudomonas stutzeri DSM 10701

35.374

100

0.364

Multiple sequence alignment