Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EJN62_RS27405 Genome accession   NZ_LR130534
Coordinates   5684604..5685056 (-) Length   150 a.a.
NCBI ID   WP_031636800.1    Uniprot ID   P17837
Organism   Pseudomonas aeruginosa isolate paerg005     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5679604..5690056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJN62_RS27385 (BANRA_05282) ampD 5680299..5680865 (-) 567 WP_023096121.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EJN62_RS27390 (BANRA_05283) - 5681016..5683271 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  EJN62_RS27395 (BANRA_05284) nadC 5683557..5684405 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EJN62_RS27405 pilA 5684604..5685056 (-) 453 WP_031636800.1 pilin Machinery gene
  EJN62_RS27410 (BANRA_05286) pilB 5685288..5686988 (+) 1701 WP_088355580.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EJN62_RS27415 (BANRA_05287) pilC 5686992..5688212 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  EJN62_RS27420 (BANRA_05288) pilD 5688216..5689088 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  EJN62_RS27425 (BANRA_05289) coaE 5689085..5689696 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  EJN62_RS27430 (BANRA_05290) yacG 5689693..5689893 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15743.11 Da        Isoelectric Point: 8.4742

>NTDB_id=1117174 EJN62_RS27405 WP_031636800.1 5684604..5685056(-) (pilA) [Pseudomonas aeruginosa isolate paerg005]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVTIKDTGDGTVKFTFATGQSSPKNAGKEITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=1117174 EJN62_RS27405 WP_031636800.1 5684604..5685056(-) (pilA) [Pseudomonas aeruginosa isolate paerg005]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAGCTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGCGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAACTATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCAAGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P17837

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

66.447

100

0.673
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45

100

0.48
  pilA Vibrio cholerae strain A1552

45

100

0.48
  pilA Vibrio cholerae C6706

45

100

0.48
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46

100

0.46
  pilA Acinetobacter baumannii strain A118

45.27

98.667

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

42.568

98.667

0.42
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

95.333

0.413
  comP Acinetobacter baylyi ADP1

41.333

100

0.413
  pilA/pilA1 Eikenella corrodens VA1

39.865

98.667

0.393
  pilA2 Legionella pneumophila str. Paris

38.255

99.333

0.38
  pilA2 Legionella pneumophila strain ERS1305867

38.255

99.333

0.38

Multiple sequence alignment