Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EJN62_RS27410 Genome accession   NZ_LR130534
Coordinates   5685288..5686988 (+) Length   566 a.a.
NCBI ID   WP_088355580.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa isolate paerg005     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5680288..5691988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJN62_RS27385 (BANRA_05282) ampD 5680299..5680865 (-) 567 WP_023096121.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EJN62_RS27390 (BANRA_05283) - 5681016..5683271 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  EJN62_RS27395 (BANRA_05284) nadC 5683557..5684405 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EJN62_RS27405 pilA 5684604..5685056 (-) 453 WP_031636800.1 pilin Machinery gene
  EJN62_RS27410 (BANRA_05286) pilB 5685288..5686988 (+) 1701 WP_088355580.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EJN62_RS27415 (BANRA_05287) pilC 5686992..5688212 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  EJN62_RS27420 (BANRA_05288) pilD 5688216..5689088 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  EJN62_RS27425 (BANRA_05289) coaE 5689085..5689696 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  EJN62_RS27430 (BANRA_05290) yacG 5689693..5689893 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  EJN62_RS27435 (BANRA_05291) - 5689930..5690139 (-) 210 WP_003094660.1 hypothetical protein -
  EJN62_RS27440 (BANRA_05292) - 5690245..5690934 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  EJN62_RS27445 (BANRA_05293) - 5690931..5691401 (-) 471 WP_023096122.1 hypothetical protein -
  EJN62_RS27450 (BANRA_05294) - 5691398..5691823 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62355.26 Da        Isoelectric Point: 5.8580

>NTDB_id=1117175 EJN62_RS27410 WP_088355580.1 5685288..5686988(+) (pilB) [Pseudomonas aeruginosa isolate paerg005]
MNDSIQLSGLSRQLVQANLLDEKTALQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
TISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQAPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1117175 EJN62_RS27410 WP_088355580.1 5685288..5686988(+) (pilB) [Pseudomonas aeruginosa isolate paerg005]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCTCTGCA
GGCGCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGTATCGCCTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
ACCATCAGCGAAAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTAGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCTGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGCCCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAGCACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGACGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.343

100

0.58
  pilB Acinetobacter baylyi ADP1

57.016

99.47

0.567
  pilB Legionella pneumophila strain ERS1305867

54.947

100

0.549
  pilB Vibrio parahaemolyticus RIMD 2210633

50.709

99.647

0.505
  pilF Neisseria gonorrhoeae MS11

50.356

99.293

0.5
  pilB Vibrio cholerae strain A1552

49.823

99.647

0.496
  pilB Vibrio campbellii strain DS40M4

49.468

99.647

0.493
  pilF Thermus thermophilus HB27

39.891

96.996

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40

92.756

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

38.764

94.346

0.366

Multiple sequence alignment