Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EJN60_RS10235 Genome accession   NZ_LR130533
Coordinates   2074738..2075190 (-) Length   150 a.a.
NCBI ID   WP_031636800.1    Uniprot ID   P17837
Organism   Pseudomonas aeruginosa isolate paerg009     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2069738..2080190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJN60_RS10215 (BANRA_01982) ampD 2070433..2070999 (-) 567 WP_023096121.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EJN60_RS10220 (BANRA_01983) - 2071150..2073405 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  EJN60_RS10225 (BANRA_01984) nadC 2073691..2074539 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EJN60_RS10235 pilA 2074738..2075190 (-) 453 WP_031636800.1 pilin Machinery gene
  EJN60_RS10240 (BANRA_01986) pilB 2075422..2077122 (+) 1701 WP_088355580.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EJN60_RS10245 (BANRA_01987) pilC 2077126..2078346 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  EJN60_RS10250 (BANRA_01988) pilD 2078350..2079222 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  EJN60_RS10255 (BANRA_01989) coaE 2079219..2079830 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  EJN60_RS10260 (BANRA_01990) yacG 2079827..2080027 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15743.11 Da        Isoelectric Point: 8.4742

>NTDB_id=1117127 EJN60_RS10235 WP_031636800.1 2074738..2075190(-) (pilA) [Pseudomonas aeruginosa isolate paerg009]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVTIKDTGDGTVKFTFATGQSSPKNAGKEITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=1117127 EJN60_RS10235 WP_031636800.1 2074738..2075190(-) (pilA) [Pseudomonas aeruginosa isolate paerg009]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAGCTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGCGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAACTATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCAAGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P17837

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

66.447

100

0.673
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45

100

0.48
  pilA Vibrio cholerae strain A1552

45

100

0.48
  pilA Vibrio cholerae C6706

45

100

0.48
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46

100

0.46
  pilA Acinetobacter baumannii strain A118

45.27

98.667

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

42.568

98.667

0.42
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

95.333

0.413
  comP Acinetobacter baylyi ADP1

41.333

100

0.413
  pilA/pilA1 Eikenella corrodens VA1

39.865

98.667

0.393
  pilA2 Legionella pneumophila str. Paris

38.255

99.333

0.38
  pilA2 Legionella pneumophila strain ERS1305867

38.255

99.333

0.38

Multiple sequence alignment